Serum concentrations of ATX were measured using a two‐site enzyme immunoassay and an automated immunoassay analyzer (Tosoh, Tokyo, Japan). Immunoassay kits were used to measure the serum concentrations of Wisteria floribunda agglutinin‐positive Mac‐2‐binding protein (WFA(+)‐M2BP; HISCL, Sysmex, Hyogo, Japan), hyaluronic acid (HA; Wako Pure Chemical Industries, Tokyo, Japan), and type IV collagen 7S (IVC7S; Sceti Medical Labo K.K., Tokyo, Japan). Additional surrogate blood indices of liver fibrosis assessed at enrollment were the fibrosis‐4 index and the aspartate aminotransferase (AST)‐to‐platelet ratio, which were calculated as follows: (age [years] × AST [IU/L]/platelet count [109/L] × alanine aminotransferase [ALT] [IU/L]1/2)23 and (AST/upper limit of normal; 40 [IU/L]) × (100/platelet count [109/L]),24 respectively.
Automated immunoassay analyzer
Manufactured by Tosoh
Sourced in Japan
The Automated Immunoassay Analyzer is a laboratory instrument used for the automated detection and quantification of various analytes in biological samples, such as proteins, hormones, and antibodies. The core function of this device is to perform immunoassay tests, which rely on the interaction between an antigen and a specific antibody to measure the concentration of the target substance.
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Lab products found in correlation
3 protocols using automated immunoassay analyzer
1
Serum Biomarkers for Liver Fibrosis
2
Porcine Hormone Quantification Assays
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All samples were analyzed in duplicate in a single batch. Plasma total triiodothyronine (TT3) and total thyroxin (TT4) concentrations were determined using commercial immunoassay kits (ST AIA-PACK T3 or T4 respectively, TOSOH) and the AIA-1800 device (Automated Immunoassay Analyzer; TOSOH). The intra-assay CV were less than 7% for the two hormones. Plasma leptin concentrations were quantified using the multispecies radioimmunoassay kit (XL-85 K; Immunodiagnostic System) previously validated for use in porcine plasma25 (link). The intra-assay CV was 3.1% for a leptin concentration of 7 ng/mL. Plasma concentrations of total ghrelin were quantified using a RIA kit (Phoenix Pharmaceuticals, Inc.) that has been used previously in pigs26 (link). The intra-assay CV was 8% at 220 pg/mL. Plasma IGF-I concentration was determined after an acid–ethanol extraction using the IRMA IGF-I kit (Beckman-Coulter-Immunotech). The intra-assay CV was 12% at 553 ng/mL.
3
Automated Immunoassay for Serum ATX Measurement
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Serum concentrations of ATX were measured using a two-site enzyme immunoassay and an automated immunoassay analyzer (Tosoh Corp., Tokyo, Japan). The assay reagent was compatible with a commercial automated immunoassay analyzer AIA System (Tosho Corp., Tokyo Japan). This system included automated 10 μL of specimen dispensation, incubation of the reaction cup, bound-free washing, 4-methylumbelliferyl phosphate substrate dispensation and fluorometric detection[8 (link)-10 (link)].
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