Tianseq stranded rna seq kit

Transcriptome library construction were performed by Hieff NGS Ultima Dual-mode mRNA Library Prep Kit for Illumina (Yeasen Biotechnology (Shanghai) Co., Ltd., China), Fast RNA-seq Lib Prep Module for Illumina (ABclonal Technology Co.,Ltd., China), TIANSeq Stranded RNA-Seq Kit (Illumina) (TIANGEN Biotech (Beijing) Co., Ltd., China) and VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina (Vazyme Biotech Co., Ltd., China). These mRNA libraries were marked as Mouse, Human or Bean_mRNA_YS, AB, TG or VZ. LncRNA library were constructed via Hieff NGS Ultima Dual-mode RNA Library Prep Kit for Illumina and Hieff NGS MaxUp rRNA Depletion Kit (human/mouse/rat) (Yeasen Biotechnology (Shanghai) Co., Ltd., China), VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina and Ribo-off rRNA Depletion Kit (Human/Mouse/Rat) (Vazyme Biotech Co.,Ltd., China), TIANSeq Stranded RNA-Seq Kit (Illumina) and TIANSeq rRNA Depletion Kit (H/M/R) (NR101-TA) (TIANGEN Biotech (Beijing) Co.,Ltd.,China). These libraries were marked as Mouse or Human_LncRNA_YS, VZ or TG. After library QC, they were subjected to NovaSeq 6000 and GenoLab M sequencing in PE150 or PE100 mode.

The total RNA extracted from twenty-day-old WT and T3 transgenic plants were used to construct the cDNA libraries by following the user’ instruction of TIANSeq Stranded RNA-Seq Kit (Illumina) (YAD, China). The libraries were sequenced with an Illumina Hiseq system at Beijing Genomics institution. Single-end sequences were first obtained, and adaptor sequences were removed from the raw sequences, and then the lower quality sequences (p < 0.05. Three biological replicates of WT and overexpression line 18, 10, 14 (each line was served as a replicate of overexpression plants) were analyzed. The RNA-sequencing data set can be obtained from SRA database with an accession number of PRJNA779571.