Bovine eye lens α crystallin

Manufactured by Merck Group

Sourced in United States

Bovine eye lens α-crystallin is a protein found in the eye lens of cattle. It is a component of the lens' cytoskeleton and plays a role in maintaining the lens' transparency and refractive index.

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Lab products found in correlation

α crystallin, by Merck Group (1 mentions) Cholestane spin label, by Merck Group (1 mentions) Cholesterol chol, by Avanti Polar Lipids (1 mentions) Hepes, by Merck Group (1 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phosphoethanolamine, by Avanti Polar Lipids (1 mentions) Sodium chloride (nacl), by Merck Group (1 mentions) Egg sphingomyelin, by Avanti Polar Lipids (1 mentions)

Close spelling variants of this product

Bovine α-crystallin α-crystallin

2 protocols using bovine eye lens α crystallin

Membrane Lipid and Protein Interactions

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DMPC (14:0–14:0 PC), SOPC (18:0–18:1 PC), DOPC (18:1–18:1 PC), PAPC (16:0–20:4 PC), and Cholesterol (Chol) were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). The cholesterol analog cholestane spin-label (CSL) and bovine eye lens α-crystallin were purchased from Sigma Aldrich (St. Louis, MO, USA), where α-crystallin was used without further purification. Based on the information (αA = 19.8 kDa, αB = 22 kDa, and αA:αB = 3:1), the average molecular weight for the α-crystallin subunit was estimated to be 20.35 kDa. Preparation of membranes, α-crystallin, and α-crystallin membrane association studies was performed in HEPES buffer (10 mM HEPES, 100 mM NaCl, pH = 7.4).

Trossi-Torres G., Timsina R, & Mainali L. (2022). Alpha-Crystallin-Membrane Association Modulated by Phospholipid Acyl Chain Length and Degree of Unsaturation. Membranes, 12(5), 455.

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Preparation and Characterization of α-Crystallin

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PLs, 1-palmitoy-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), 1-palmitoy-2-oleoyl-sn-glycero-3-phosphatidylserine (POPS), and egg sphingomyelin (SM) were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). Cholesterol analog cholestane spin-label CSL was purchased from Sigma Aldrich (St. Louis, Mo, USA). Bovine eye lens α-crystallin, HEPES, and Sodium Chloride were purchased from Sigma Aldrich (St. Louis, MO, USA). The α-crystallin (C4163) obtained from Sigma Aldrich was used without further purification. Based on the information provided by the Sigma Aldrich (i.e., αA = 19.8 kDa, αB = 22 kDa, and αA:αB = 3:1), the average molecular weight of α-crystallin subunit was estimated to be 20.35 kDa.

Timsina R., Khadka N.K., Maldonado D, & Mainali L. (2020). Interaction of Alpha-Crystallin with Four Major Phospholipids of Eye Lens Membranes. Experimental eye research, 202, 108337.

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