Hepes minimum 99.5 titration

Dimethylsulfoxide, quercetin, 5,5′-dithio-bis (2-nitrobenzoic acid), acetylcholine iodide, 1-chloro-2,4-dinitrobenzene, 2′,7′-dichlorofluorescein diacetate (DCHF-DA), Folin-Ciocalteu, HEPES minimum 99.5% titration, 2,4,6–tris(2-pyridyl)-5-triazine (TPTZ), resazurin sodium salt, albumin from bovine serum (BSA), sucrose, reduced glutathione, tetramethylethylenediamine, and quercetin were purchased from Sigma-Aldrich (São Paulo, SP, Brazil). DNAse I Amplification Grade, SYBR Select Master Mix, and TRIzol were obtained from Life Technologies; iScript cDNA Synthesis kit was obtained from Biorad (CA, USA). Griess reagent system and Caspase-Glo 3/7 and caspase 9 were purchased from Promega (WI, USA). Mancozeb (80% purity, Enzeb 800 WP) was purchased from Sabero Organics America SA (Belo Horizonte, MG, Brazil). All other reagents were commercial products of the highest purity grade available.

Dimetil sulfoxide (DMSO), Folin-Ciocalteu, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid diammonium salt, sodium acetate, HEPES minimum 99.5% titration, albumin from bovine serum (BSA), reduced glutathione (GSH), oxidized glutathione, tetramethylethylenediamine (TEMED), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), D-Manitol, K₂HPO₄, KH₂PO₄, Triton X-100, β-mercaptoethanol, anti-rabbit immunoglobulin (HRP peroxidase-linked antibody), and carbonyl cyanide 4-(trifluoromethoxy) phenylhydrate (FCCP) were obtained from Sigma-Aldrich (São Paulo, SP, Brazil). SDS, acrylamide, bis-acrylamide, and hybond nitrocellulose were obtained from GE Healthcare Life Division (Uppsala, Sweden). Anti-phospho-p38 (Thr180/Tyr182) and total form, anti phospho-AKT (Thr308) and total form, anti-phospho PTEN, anti-phospho and total JNK1/2 (Thr183/Tyr185), anti-phospho ERK1/2 (Thr202/Tyr204) and anti-total-ERK1/2, and β-actin antibodies were purchased from Cell Signaling Technology (Danvers, MA). Poly (ADP)-ribose polymerase (PARP) antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Kit Caspase-Glo 3/7 was obtained from Promega (Madison, WI). All other reagents were commercial products of the highest purity grade available.