Zileuton

Manufactured by Bio-Techne

Sourced in United States

Zileuton is a laboratory product manufactured by Bio-Techne. It is a 5-lipoxygenase (5-LO) inhibitor, which functions by blocking the conversion of arachidonic acid to leukotrienes. The core function of Zileuton is to serve as a research tool for studying the role of 5-LO and leukotrienes in various biological processes.

Automatically generated - may contain errors

Lab products found in correlation

Recombinant murine ifn β, by R&D Systems (1 mentions) Recombinant human ifn β, by Merck Group (1 mentions) Il 1β, by R&D Systems (1 mentions) Il 1α, by R&D Systems (1 mentions) Recombinant human vegf, by R&D Systems (1 mentions) Iberiotoxin ibtx, by Merck Group (1 mentions) Ns1619, by Merck Group (1 mentions) Arachidonic acid, by Merck Group (1 mentions) Mead acid, by Cayman Chemical (1 mentions) Ferrostatin 1, by Cayman Chemical (1 mentions) Bwa4c, by Merck Group (1 mentions) Erastin, by Cayman Chemical (1 mentions) α tocopherol, by Merck Group (1 mentions) Dihomo γ linolenic acid, by Cayman Chemical (1 mentions) Bay x1005, by Bio-Techne (1 mentions) Sc236, by Bio-Techne (1 mentions) Pd146176, by Bio-Techne (1 mentions) Aa 861, by Merck Group (1 mentions) Psi 7977 sofosbuvir, by ChemScene (1 mentions) Sc 560, by Bio-Techne (1 mentions)

7 protocols using zileuton

Macrophage Differentiation and Mycobacterial Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols

Murine bone-marrow cells were cultured for 7 days in either 15% GM-CSF media to generate BMDC or 30% L929 supernatant media to differentiate BMDM and then exposed to H37Rv at an indicated multiplicity of infection (m.o.i.) for 24–48 h and supernatants harvested. In some experiments PGE2 (2 µg ml⁻¹, Sigma), zileuton (20 µM, Tocris), valdecoxib (0.5 µM, Tocris) or recombinant murine IFNβ (20 ng ml⁻¹), IL-1αor IL-1β(10 ng ml⁻¹) or both together (5 ng ml⁻¹ + 5 ng ml⁻¹, R&D Systems, Minneapolis, MN) were added to the cultures.
Human elutriated monocytes were obtained from peripheral blood of healthy cytomegalovirus-negative donors. Monocyte derived macrophages (MDM) were generated by culturing monocytes with media containing M-CSF (60 ng ml⁻¹) for 7 days. Fresh media with indicated growth factors was added every 48 h. Cells were exposed to H37Rv (m.o.i. = 5) in the presence or absence of PGE2 (2 µg ml⁻¹, Sigma), recombinant human IFN-β (10 ng ml⁻¹), IL-1α (10 ng ml⁻¹), IL-1β (10 ng ml⁻¹) or neutralizing anti-hIL1-R1 antibody (20 µg ml⁻¹, R&D Systems, Minneapolis, MN) for 24 h. All recombinant human cytokines were from Peprotech (Rocky Hill, NJ).

Mayer-Barber K.D., Andrade B.B., Oland S.D., Amaral E.P., Barber D.L., Gonzales J., Derrick S.C., Shi R., Kumar N.P., Wei W., Yuan X., Zhang G., Cai Y., Babu S., Catalfamo M., Salazar A.M., Via L.E., Barry CE I.I.I, & Sher A. (2014). Host-directed therapy of tuberculosis based on interleukin-1 and type I interferon crosstalk. Nature, 511(7507), 99-103.

+ Open protocol

+ Expand

Evaluating Inflammatory Responses in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Zileuton was purchased from Tocris Bioscience (Ellisville, MO, USA). NS1619 and iberiotoxin (IBTX) were obtained from Sigma (St. Louis, MO, USA). Recombinant human VEGF was acquired from R&D Systems (Minneapolis, MN, USA). BrdU assay kit (colorimetric), LTB4 kits, and both of cysteinyl leukotrienes (Cys-LTs) kits and caspACE™ assay kit were obtained from Roche applied science (Penzberg, Germany), Enzo Life Sciences (Seoul, Korea), and Abcam (Cambridge, MA, USA), respectively. PGE₂ and 6-keto PGF₁_α (the stable hydrolysis products of PGI₂) were from Enzo Life Science (Seoul, Korea) and Cayman chemicals (Ann Arbor, MI, USA). Specific antibodies against ICAM-1, VCAM-1, eNOS^Ser1179 and Erg1/2/3, Bcl-2 and Bax were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). All other reagents were purchased from Sigma (St. Louis, MO, USA) unless otherwise noted.

Lim H.J., Park J., Um J.Y., Lee S.S, & Kwak H.J. (2019). Zileuton, a 5-Lipoxygenase Inhibitor, Exerts Anti-Angiogenic Effect by Inducing Apoptosis of HUVEC via BK Channel Activation. Cells, 8(10), 1182.

+ Open protocol

+ Expand

Lipoprotein Depletion and Lipid Signaling Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

AA-861, BWA4C, α-tocopherol, linoleic acid, arachidonic acid, dimethyl sulfoxide were from Sigma. CAY10566, Mead acid, dihomo-γ-linolenic acid, erastin, ferrostatin-1, and (1S,3R)-RSL3 were from Cayman Chemical. SC560, SC236, MK886, Zileuton, BAY-X1005, PD146176, and deferoxamine were from Tocris Bioscience. PSI-7977 (Sofosbuvir) and Glecaprevir were from Chemscene. Cell viability was determined using Cell Counting Kit-8 (DOJINDO, Japan). Lipoprotein-deprived serum (LPDS) was prepared by incubating the heat-inactivated FBS with fumed silica (Sigma, S5130) overnight, followed by removal of the silica by centrifugation at 2,000g for 20 min and filtration using a 0.22 μm filter device.
Primary antibodies to SCD (1:500 dilution, #2438) was from Cell Signaling Technology; FADS1 (1:1,000 dilution, #27533) was from Cayman Chemical; FADS2 (1:1,000 dilution, A10270) were from ABclonal; FADS2 (1:1,000 dilution, 28034–1-AP) was from Proteintech; HCV NS3 (1:500, ab13830) was from Abcam; GAPDH was from Wako (1:4,000 dilution, 016–25523). IRDye 680 or 800 secondary antibodies including #926–32211, #926–32212, #926–32214, #926–68020 and #926–68073 (1:20,000) were from LI-COR.

Yamane D., Hayashi Y., Matsumoto M., Nakanishi H., Imagawa H., Kohara M., Lemon S.M, & Ichi I. (2021). FADS2-dependent fatty acid desaturation dictates cellular sensitivity to ferroptosis and permissiveness for hepatitis C virus replication. Cell chemical biology, 29(5), 799-810.e4.

+ Open protocol

+ Expand

Ferroptosis Inducers and Inhibitors

Check if the same lab product or an alternative is used in the 5 most similar protocols

Erastin (S7242) and RSL3 (S8155), Lip-1 (S7699), VBIT-4 (S3544), Mitoquinone mesylate (MitoQ, S8978), Decylubiquione (DecylQ, D7911) were purchased from Selleck Chemicals (Houston, TX, USA). Fer-1 (SML0583), Deferoxamine (DFO, D9533), Trolox (238813), z-VAD-FMK (V116), ML385 (SML1833), MitoTEMPO (SML0737) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Zileuton (3308) was from Tocris Bioscience (Bristol, UK). The following antibodies were used: GPx4 (ab125066, Abcam, Cambridge, UK) and Nrf2 (ab62352, Abcam), COXIV (4844, Cell signaling Technology, Danvers, MA, USA), FLAG (F3165, Sigma-Aldrich), α-tubulin (sc-8035, Santa Cruz, Dallas, TX, USA) and β-actin (sc-47778, Santa Cruz).

Oh S.J., Ikeda M., Ide T., Hur K.Y, & Lee M.S. (2022). Mitochondrial event as an ultimate step in ferroptosis. Cell Death Discovery, 8, 414.

+ Open protocol

+ Expand

Zileuton Attenuates MWCNT-Induced Inflammation

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

C57BL/6 mice were treated with either vehicle (0.5% methyl-cellulose/0.2% Tween 80 in water) or Zileuton, a 5-lipoxygenase inhibitor (10 mg/Kg, Tocris Bioscience, Minneapolis, MN, United States), by oropharyngeal instillation 3 h before administration of FA21 MWCNT (50 μg). Zileuton treatment was based on the concentration (10 mg/Kg) and protocol used previously by Clarke et al. (2014) (link). Control mice comprised of mice treated with carrier alone. BALF was collected 24 h after MWCNT exposure and the level of inflammation elicited was evaluated by monitoring EPO and cysLT levels.

Carvalho S., Ferrini M., Herritt L., Holian A., Jaffar Z, & Roberts K. (2018). Multi-Walled Carbon Nanotubes Augment Allergic Airway Eosinophilic Inflammation by Promoting Cysteinyl Leukotriene Production. Frontiers in Pharmacology, 9, 585.

+ Open protocol

+ Expand

Cytosolic GPX4 Mutant Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

The compound selected from virtual screen was purchased from SPECS with purity of more than 95% (confirmed by SPECS with NMR and LC-MS; data are available on the website http://www.specs.net/). Reagents were purchased from Sigma–Aldrich unless otherwise noted. The pQE-30 bacterial expression plasmid of the U46C mutant of human cytosolic GPX4 (c-GPX4) was a generous gift from Professor Hartmut Kuhn (University Medicine Berlin-Charité, Germany). IPTG, PMSF, DTT, EDTA, and glutathione were from Amresco. Standard compounds for LC-MS/MS were purchased from Cayman Chemical. Calcium ionophore A23187 was obtained from J&K Chemical. Zileuton was purchased from Tocris Bioscience. The Symmetry C18 reverse-phase column (3.5 μm, 2.1 mm × 150 mm) was purchased from Waters Corp. HEK293T cells were received as a gift from Professor Jincai Luo (Peking University, China). The Dual-Glo Luciferase Assay System was from Promega. The pCDNA3.1 plasmid containing the gene encoding firefly luciferase was a generous gift from Professor Peng Chen (Peking University, China). Antibody against GPX4 was obtained from Abcam (no. ab125066). HT-1080 cells were the generous gift of Professor Chu Wang (Peking University, China).

Li C., Deng X., Xie X., Liu Y., Friedmann Angeli J.P, & Lai L. (2018). Activation of Glutathione Peroxidase 4 as a Novel Anti-inflammatory Strategy. Frontiers in Pharmacology, 9, 1120.

+ Open protocol

+ Expand

Arachidonic Acid Metabolism Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human recombinant COX-1, COX-2, 5-LOX, and arachidonic acid (AA) were from Cayman Chemical (Ann Arbor, MI). Cell culture reagents were from American Type Culture Collection or Invitrogen. Collagen fibrils (type I) from equine tendons were obtained from Chrono-Log Corp (Havertown, PA). Zileuton (a 5-LOX inhibitor) was purchased from Tocris Cookson (Minneapolis, MN). The primary and secondary antibody for detecting 5-LOX were purchased from BD Biosciences Pharmingen and Santa Cruz Biotechnology, respectively. Human recombinant IL-1β, bacterial lipopolysaccharide (LPS), and all other chemicals were from Sigma (St. Louis, MO).

Park N.Y., Im S, & Jiang Q. (2021). Different forms of vitamin E and metabolite 13’-carboxychromanols inhibit cyclooxygenase-1 and its catalyzed thromboxane in platelets, and tocotrienols and 13’-carboxychromanols are competitive inhibitors of 5-lipoxygenase. The Journal of nutritional biochemistry, 100, 108884.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!