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Pierce nuclear protein extraction kit

Manufactured by Thermo Fisher Scientific
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The PIERCE Nuclear Protein Extraction Kit is a laboratory product designed to isolate and extract nuclear proteins from mammalian cells. It provides a simple and efficient method for obtaining nuclear protein fractions for further analysis and applications.

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Nuclear extraction kit (54 citations) Protein extraction kit (53 citations)

2 protocols using pierce nuclear protein extraction kit

1

Nuclear Protein Extraction Protocol

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Nuclear protein was extracted using the PIERCE Nuclear Protein Extraction Kit (Thermo), following the manual. Briefly, cells were collected by centrifugation, 500g for 3 min at 4°C. Resuspend cells in 200μl precooled CER I by 15 sec of intense vortex, and incubate on ice for 10 min. Add 11μl precooled CER II to the solution, and after 5 sec of intense vortex, incubate it on ice for 1 min. After 5 sec of intense vortex, centrifuge the solution at 16000g for 5 min, at 4°C, discard the supernatant. Add 100μl NER to the precipitate and vibrate it intensively for 15 sec, and incubate it on ice for 10 min. Repeat this step 4 times, and then centrifuge the solution at 16000g for 10 min, at 4°C. The supernatant was purified nuclear protein.
Li B., Jie W., Huang L., Wei P., Li S., Luo Z., Friedman A.K., Meredith A.L., Han M.H., Zhu X.H, & Gao T.M. (2014). Nuclear BK Channels Regulate Gene Expression via the Control of Nuclear Calcium Signaling. Nature neuroscience, 17(8), 1055-1063.
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2

Nuclear Protein Extraction Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Nuclear protein was extracted using the PIERCE Nuclear Protein Extraction Kit (Thermo), following the manual. Briefly, cells were collected by centrifugation, 500g for 3 min at 4°C. Resuspend cells in 200μl precooled CER I by 15 sec of intense vortex, and incubate on ice for 10 min. Add 11μl precooled CER II to the solution, and after 5 sec of intense vortex, incubate it on ice for 1 min. After 5 sec of intense vortex, centrifuge the solution at 16000g for 5 min, at 4°C, discard the supernatant. Add 100μl NER to the precipitate and vibrate it intensively for 15 sec, and incubate it on ice for 10 min. Repeat this step 4 times, and then centrifuge the solution at 16000g for 10 min, at 4°C. The supernatant was purified nuclear protein.
Li B., Jie W., Huang L., Wei P., Li S., Luo Z., Friedman A.K., Meredith A.L., Han M.H., Zhu X.H, & Gao T.M. (2014). Nuclear BK Channels Regulate Gene Expression via the Control of Nuclear Calcium Signaling. Nature neuroscience, 17(8), 1055-1063.
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