Anti cleaved parp mab

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-cleaved PARP mAb is a mouse monoclonal antibody that specifically recognizes the cleaved form of Poly(ADP-ribose) Polymerase (PARP). PARP is a nuclear enzyme involved in DNA repair, and its cleavage is a hallmark of apoptosis. This antibody can be used to detect apoptosis in cells and tissues.

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Lab products found in correlation

Anti bax mab, by Cell Signaling Technology (1 mentions) Ripa buffer, by Beyotime (1 mentions) Anti β actin, by ZSGB-BIO (1 mentions) Astaxanthin, by Merck Group (1 mentions) Anti bcl2 mab, by Cell Signaling Technology (1 mentions) Ros detection assay kit, by Abcam (1 mentions) Anti bax pab, by Cell Signaling Technology (1 mentions) Cell counting kit 8 (cck8), by Dojindo Laboratories (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Pe annexin 5 apoptosis detection kit, by BD (1 mentions)

Close spelling variants of this product

Anti-cleaved PARP Anti-PARP mAb Cleaved PARP Anti-PARP

2 protocols using anti cleaved parp mab

Apoptosis Signaling Pathway Analysis

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Cells were lysed in RIPA buffer (Beyotime, Shanghai, China) supplemented with protease inhibitors in ice for 30 min followed by centrifugation at 12,000 × g for 15 min at 4°C. Immunoblotting was performed using the anti-cleaved PARP mAb (1:1,000), anti-Bax mAb (1:1,000; Cell Signaling Technology, Danvers, MA, USA), anti-cytochrome c mAb (1:500), anti-caspase-3 mAb (1:1,000; Abcam, Cambridge, MA, USA), anti-HBc mAb (1:500; Milipore, Billerica, MA, USA), anti-β-actin and anti-secondary antibodies were purchased from ZSGB-BIO (Beijing, China).

Gao W.Y., Li D., Cai D.E., Huang X.Y., Zheng B.Y., Huang Y.H., Chen Z.X, & Wang X.Z. (2016). Hepatitis B virus X protein sensitizes HL-7702 cells to oxidative stress-induced apoptosis through modulation of the mitochondrial permeability transition pore. Oncology Reports, 37(1), 48-56.

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Astaxanthin-Induced Apoptosis and ROS Modulation

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Astaxanthin and dimethyl sulfoxide were purchased from Sigma-Aldrich Inc. (Taufkichen, Germany). Cell Counting Kit-8 (CCK-8) was purchased from DOJINDO Inc. (Kumamoto, Japan). ROS Detection Assay Kit was purchased from Biovision Inc. (Milpitas, CA, USA). PE Annexin V apoptosis detection kit was purchased from BD Biosciences Inc. (San Jose, CA, USA). Anti-BAX pAb, anti-BCL2 mAb, anti-cleaved CASP3 mAb, and anti-cleaved PARP mAb were purchased from Cell Signaling Technology Inc. (Danvers, MA, USA). Anti-ACTB pAb were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Chung B.Y., Park S.H., Yun S.Y., Yu D.S, & Lee Y.B. (2022). Astaxanthin Protects Ultraviolet B-Induced Oxidative Stress and Apoptosis in Human Keratinocytes via Intrinsic Apoptotic Pathway. Annals of Dermatology, 34(2), 125-131.

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