Mcolorphast

Manufactured by Merck Group

Sourced in Germany

MColorpHast is a pH test strip product line offered by Merck Group. The test strips are designed to quickly and easily determine the pH value of a solution. The product provides a simple and reliable way to measure pH levels across a wide range.

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Lab products found in correlation

Infinite m200 pro, by Tecan (2 mentions) Atx tris buffer, by Merck Group (1 mentions) Unity 500, by Agilent Technologies (1 mentions) Xylocaine, by AstraZeneca (1 mentions) Surepath liquid based pap test, by BD (1 mentions) Accu chek aviva blood glucose meter system, by Roche (1 mentions) Accu chek aviva test strip, by Roche (1 mentions)

16 protocols using mcolorphast

Measuring and Adjusting Solution pH

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Solution pH was measured using the pH strips indicator MColorpHast^® product available at Merck Millipore (Watford, UK). The pH was adjusted using a 1 M NaOH or 1 M HCl solution prepared respectively from NaOH pellet (99%) and HCl (36.5%) purchased from VWR Chemicals. In one particular experiment, the reaction mixture was buffered to pH 9 using the ATX Tris-buffer (ready to use solution) purchased from Sigma-Aldrich.

Zecchini M., Lucas R, & Le Gresley A. (2019). New Insights into the Cystine-Sulfite Reaction. Molecules, 24(13), 2377.

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NMR Characterization of EE2-Conjugated Dendrimer

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~20 EE₂-conjugated EDC (32 mg) was dissolved into 0.65 mL D₂O, followed by adjusting pH with 35 wt% 0.1 N DCl in D₂O and 40 wt% 0.1N NaOD in D₂O using a micro syringe, based on the total number of amines on the dendrimer. (pH values were confirmed by pH-indicator paper; non-bleeding pH 5.0 to 10.0, MColorpHast™, EMD Millipore) To minimize magnetic field inhomogeneity, spectra were obtained from the same NMR tube. T1 and T2 values were determined on a Varian Unity-500 narrow bore NMR spectrometer using a Hahn echo decay experiment and an inversion-recovery method, respectively, using internally installed software. Correlation times were determined by the polynomial function τ_c(ns) = a0 + a1(R2/R1) + a2(R2/R1)² + a3(R2/R1)³ + a4(R2/R1)⁴, where R1/R2 = T2/T1, and the values for a0, a1, a2, a3, and a4 were taken from the literature³⁶.

Kim S.H., Madak-Erdogan Z., Bae S.C., Carlson K.E., Mayne C.G., Granick S., Katzenellenbogen B.S, & Katzenellenbogen J.A. (2015). Ligand Accessibility and Bioactivity of a Hormone-Dendrimer Conjugate Depend on pH and pH History. Journal of the American Chemical Society, 137(32), 10326-10335.

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Intubation and Positioning of Naso-ileal Catheter

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A 305 cm long silicon 9-lumen (8-lumen, 1 balloon inflation channel, the outer diameter of 3.5 mm) custom-made naso-ileal reusable catheter (Dentsleeve International, Mui Scientific, Mississauga, Canada) was used for intubation.
One day prior to the first test day, subjects arrived at 7:40 AM at the Maastricht University Medical Center+ (MUMC+) after an overnight fast. If preferred by the subject, local anesthesia of nasal mucosa using xylocaine (10% spray, AstraZeneca, Zoetermeer, The Netherlands) was applied. After placement of the catheter in the stomach, the catheter was guided through the pylorus and into the duodenum under intermittent fluoroscopic control. Progression of the catheter from duodenum to ileum was performed as described earlier [19 (link)]. Fluoroscopy was used to check the positioning of the catheter on the first and the last test day. Radio-opaque markers were added to the infusion ports on the catheter, which accounted for the determination of the catheter position. On all test days, intestinal fluid was sampled from various infusion ports, and pH was measured using pH strips (MColorpHast™, Merck, Darmstadt, Germany) in order to estimate the catheter positioning.

Klaassen T., Alleleyn A.M., van Avesaat M., Troost F.J., Keszthelyi D, & Masclee A.A. (2019). Intraintestinal Delivery of Tastants Using a Naso-Duodenal-Ileal Catheter Does Not Influence Food Intake or Satiety. Nutrients, 11(2), 472.

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Vaginal Epithelial Sampling Protocol

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Vaginal samples were collected by trained gynecologists. The procedure began by removing the cervical mucus with a sterile swab. Furthermore, endocervical and exocervical epithelia were gathered with a cervix examination brush (Rovers Cervex-Brush; Rovers Medical Devices B.V., Oss, The Netherlands). Additionally, the pH of the mucus samples was determined from the vaginal swab using a pH test strip (MColorpHast; Merck-Millipore, Burlington, MA, USA). The detachable head of the cervix examination brush was preserved in a liquid vial (BD SurePath™ liquid-based Pap test; BD Biosciences, San Jose, CA, USA) and transported under refrigeration conditions (4 °C). The samples were stored at −40 °C for 5 h until DNA extraction.

Salinas A.M., Osorio V.G., Endara P.F., Salazar E.R., Vasco G.P., Vivero S.G, & Machado A. (2018). Bacterial identification of the vaginal microbiota in Ecuadorian pregnant teenagers: an exploratory analysis. PeerJ, 6, e4317.

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Glucose and pH Monitoring of Cell-Free Supernatant

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Determinations of glucose concentration and pH value of the cell-free supernatant were performed in parallel every 2–3 days using blood glucose meter Accu Check Aviva and pH indicator strips for a pH range between 6.5 and 10 (MColorpHast™; Merck).

Oberschmidt O., Morgan M., Huppert V., Kessler J., Gardlowski T., Matthies N., Aleksandrova K., Arseniev L., Schambach A., Koehl U, & Kloess S. (2019). Development of Automated Separation, Expansion, and Quality Control Protocols for Clinical-Scale Manufacturing of Primary Human NK Cells and Alpharetroviral Chimeric Antigen Receptor Engineering. Human Gene Therapy Methods, 30(3), 102-120.

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Antibacterial effects of AEA

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Bacterial suspensions of OD_600nm = 0.1 in BHI medium were treated with different concentrations of AEA (1.25–50 μg/mL) and incubated at 37 °C, air/5% CO₂ for 24 h. At various time points, the pH of the samples was measured using pH indicator paper strips (MColorpHast, Merck KGaA, Darmstadt, Germany), and the optical density at 595 nm was measured in parallel in a Tecan Infinite M200 PRO plate reader (Tecan Trading AG, Männedorf, Switzerland) [20 (link)].

Wolfson G., Sionov R.V., Smoum R., Korem M., Polacheck I, & Steinberg D. (2023). Anti-Bacterial and Anti-Biofilm Activities of Anandamide against the Cariogenic Streptococcus mutans. International Journal of Molecular Sciences, 24(7), 6177.

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Vaginal pH Measurement with Indicator Strips

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The pH measurement was performed with the use of the MColorpHast™ indicator strips (Merck, Darmstadt, Germany) to which the tip of the vaginal speculum was applied immediately after removing it from the vagina. The pH was evaluated 2 s. after, according to the manufacturer’s recommendations.

Golińska E., Sowińska N., Tomusiak-Plebanek A., Szydło M., Witka N., Lenarczyk J, & Strus M. (2021). The vaginal microflora changes in various stages of the estrous cycle of healthy female dogs and the ones with genital tract infections. BMC Veterinary Research, 17, 8.

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Evaluating Bacterial Penetration of Dentin Tubules

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To see whether the bacteria would penetrate the dentin rings through the dentinal tubules, S. mutans labeled with green fluorescence protein (GFP) [25 (link)] was used for the biofilm challenge in a separate test. The fluorescent S. mutans was cultured in BHI liquid medium and diluted as described above. Again, different concentrations of sucrose were used to adjust the pH of the medium (Table 1). The pH of the medium was measured using pH-indicator strips (CAT# 1095420001, MColorpHast™, EMD Millipore). For simplicity, the dentin rings were placed within the wells of a 24-well plate and incubated in a 5% CO₂ incubator maintained at 37 °C. Each dentin disc was challenged with 2 mL of the medium, which was replaced every 24 hrs. The sample size for each combination of pH and duration was 3. The rings thus tested were imaged with a fluorescence microscope to estimate the depth of bacterial penetration.

Zhang A., Chen R., Aregawi W., He Y., Wang S., Aparicio C.J., Rudney J., Chew H.P, & Fok A.S. (2020). Development and Calibration of Biochemical Models for Testing Dental Restorations. Acta biomaterialia, 109, 132-141.

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Effect of CBG on Streptococcus mutans

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Streptococcus mutans of OD₆₀₀_nm = 0.1 was treated with different concentrations of CBG (0, 2.5, 5, and 10 μg/ml) and incubated at 37°C for 24 h. At various time points, the pH of the samples was measured using pH-indicator strips (MColorpHast, Merck KGaA, Darmstadt, Germany).

Aqawi M., Sionov R.V., Gallily R., Friedman M, & Steinberg D. (2021). Anti-Bacterial Properties of Cannabigerol Toward Streptococcus mutans. Frontiers in Microbiology, 12, 656471.

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Glucose and pH Measurement in Media

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To measure glucose and pH in media, 1 mL of either fresh or spent media was added to a 1.5 mL tube.
A droplet (7.5 μL) of media was placed on an Accu-Chek Aviva Test Strip and read with an Accu-Chek Aviva Blood Glucose Meter System (Roche Diagnostics, Mannheim, Germany). The linear range of the Accu-Chek Aviva Blood Glucose Meter System from 11 mM downwards had been determined previously and found to be in accordance to manufacturer specifications (0.55–33 mM) although the minimum glucose concentration reliably measured was 0.7 mM.
pH of media was measured by addition of 70 μL of media to a non-bleeding pH 2.0–9.0 indicator strip (MColorpHast, Merck Millipore, Darmstadt, Germany).

Wright Muelas M., Ortega F., Breitling R., Bendtsen C, & Westerhoff H.V. (2018). Rational cell culture optimization enhances experimental reproducibility in cancer cells. Scientific Reports, 8, 3029.

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