Fuji bio imaging analyzer fla 7000

Manufactured by Fujifilm

Sourced in Japan

The Fuji Bio-Imaging Analyzer FLA-7000 is a versatile laboratory instrument designed for the detection and analysis of various biomolecules, including proteins, nucleic acids, and radioactive samples. The instrument utilizes a high-sensitivity fluorescence detection system and advanced image processing software to provide accurate and reliable results.

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Lab products found in correlation

Multi gauge v3, by Fujifilm (2 mentions) Superfrost plus glass slide, by Thermo Fisher Scientific (2 mentions) Bas tr2025, by Fujifilm (2 mentions) Adult female lewis rats, by Charles River Laboratories (1 mentions) Microm cryostat, by Thermo Fisher Scientific (1 mentions) Microm cryotome, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

FLA-7000 (98 citations) FLA-7000 imaging analyzer (12 citations) FUJI FLA-7000 (6 citations) FLA-7000 analyzer (5 citations) FLA 7000 Bio-Imaging Analyzer (2 citations) Fuji imaging analyzer (2 citations)

8 protocols using fuji bio imaging analyzer fla 7000

Measuring Spinal Cord Tracer Uptake in EAE Rats

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To assess the difference of tracer uptake in rat spinal cord, 20 μm slices from EAE and sham rats were incubated with 2.8 MBq/mL [¹¹C]GSK1482160 at room temperature for 30 min, following by a 15-min pre-incubation in 50 mM Tris–HCl buffer (pH 7.4). Following this incubation, sections were washed 2 times in assay buffer at 4 °C and rinsed once in distilled water at 4 °C to remove buffer salts. Then the slides were air-dried and exposed to the storage phosphor screen in an imaging cassette for 2 – 4 hrs in −20° C at dark. The distribution of radioactivity was visualized by a Fuji Bio-Imaging Analyzer FLA-7000 (Fuji Photo Film, Tokyo, Japan). Photo-stimulated luminescence was quantified using Multi Gauge v3.0 software (Fuji Photo Film, Tokyo, Japan). Data were background-corrected and expressed as photo-stimulated luminescence signals per square millimeter (PSL/mm²).

Han J., Liu H., Liu C., Jin H., Perlmutter J.S., Egan T.M, & Tu Z. (2017). Pharmacologic characterizations of a P2X7R-specific radioligand, [11C]GSK1482160 for neuroinflammatory response. Nuclear medicine communications, 38(5), 372-382.

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Evaluating [11C]TZ3321 Binding Specificity

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To confirm the specificity of the radiotracer [¹¹C]TZ3321 binding toward S1PR1, in vitro autoradiography study was conducted. Twenty micrometer slices from snap-frozen femoral plaque tissues were incubated with 2 µCi/ml [¹¹C]TZ3321 at room temperature for 30 min, following a 15-min pre-incubation in the binding buffer (50 mM HEPES-Na (pH 7.5), 5 mM MgCl₂, and 1 mM CaCl₂, 0.5 % fatty acid-free BSA). A S1PR1-specific compound SEW2871 (10 µM) was used as a block agent to check the binding specificity of [¹¹C]TZ3321 toward S1PR1. Following this incubation, sections were washed two times in assay buffer at 4 °C and rinsed once in distilled water at 4 °C to remove buffer salts. Then, the slides were dried and exposed to the storage phosphor screen (BAS-IP-MS-2025) in an imaging cassette for 4 h in − 20 °C at dark. The distribution of radioactivity was visualized by a Fuji Bio-Imaging Analyzer FLA-7000 (Fuji Photo Film, Tokyo, Japan).

Liu H., Jin H., Han J., Yue X., Yang H., Zayed M.A., Gropler R.J, & Tu Z. (2018). Upregulated Sphingosine 1-Phosphate Receptor 1 Expression in Human and Murine Atherosclerotic Plaques. Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, 20(3), 448-456.

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Evaluating S1P1 Binding Specificity of [18F]12a

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Example 54

To check the binding specificity of [¹⁸F]12a toward S1P1 in the brain, in vitro autoradiography study was performed using rat brain slices. Adult female Lewis rats (n=2, 100-125 g, Charles River) were used to perform the autoradiography study. The rats (n=2) was euthanized and the brains were collected and cut into 20 μm sequential sections using a Microm cryotome and mounted on glass slides. For the control study, the slides were incubated with ˜6 nM of [¹⁸F]12a at RT for 60 min. For the blocking studies, slides were incubated with [¹⁸F]12a in the presence of 10 μM of SEW2871 at RT for 60 min. Following the incubation, the brain sections were washed and exposed to the Storage Phosphor Screen in an imaging cassette overnight in −20° C. at dark for 12 h. The distribution of radioactivity was visualized by a Fuji Bio-Imaging Analyzer FLA-7000 (Fuji Photo Film, Tokyo, Japan). Photo-stimulated luminescence (PSL) from the brain slices was quantified using Multi Gauge v3.0 software (Fuji Photo Film Co., Tokyo, Japan). Data were background-corrected, and expressed as photo-stimulated luminescence signals per square millimeter (PSL/mm²). In vitro autoradiography study showed that SEW2871 was able to reduce ˜30% of radioactivity, demonstrating that [¹⁸F]12a has specific binding with S1P1 (FIG. 3).

US10676467B2. Compositions for binding sphingosine-1-phosphate receptor 1 (S1P1), imaging of S1P1, and methods of use thereof (2020-06-09). None [US], None [US], None [US], None [CN]. Inventors: Zhude Tu [US], Adam Rosenberg [US], Hui Liu [US], Junbin Han [CN].

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Ex vivo Autoradiography of Novel PET Ligands

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For the ex vivo autoradiography study, mature male SD rats (~250 g) were injected with ~20 MBq of (-)-[¹¹C]8 or (-)-[¹⁸F]14a in 10% ethanol in saline via the tail vein under isoflurane/oxygen anaesthesia. The rats were euthanized after 30 min for (-)-[¹¹C]8 or 60 min for (-)-[¹⁸F]14a, and the brains were quickly removed and frozen. The frozen brains were then sectioned into 100 μm transverse slices and mounted on glass slides. Frozen slides were directly exposed to a BAS storage phosphor screen film (BAS-IP-MS-2025) in an imaging cassette (Fuji Photo Film Co., Tokyo, Japan) for 12 h at −80 °C under the dark. The distribution of radioactivity was visualized by a Fuji Bio-Imaging Analyzer FLA-7000 (Fuji Photo Film Co., Tokyo, Japan). Photo-stimulated luminescence (PSL) from the striatum and cerebellum was quantified using Multi Gauge v3.0 software (Fuji Photo Film Co., Tokyo, Japan). Data were background-corrected and expressed as photo-stimulated luminescence signals per square millimeter (PSL/mm²), then normalized to provide the ratio of striatum to cerebellum.

Luo Z., Liu H., Jin H., Gu J., Yu Y., Kaneshige K., Perlmutter J.S., Parsons S.M, & Tu Z. (2018). Exploration of new sulfur-containing analogues for imaging vesicular acetylcholine transporter in the brain. ChemMedChem, 13(18), 1978-1987.

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In Vivo Radiotracer Imaging of Rat Brain

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Rats (n = 2) were anesthetized with 2-3% isoflurane/oxygen, and were injected with [¹¹C]TZ1964B (~ 74 MBq) via the tail vein. At 30 min post injection, rats were euthanized and the whole brains were dissected. Intact brains were flash frozen in dry ice, pre-cooled in isopentane and stored on dry ice. Horizontal sections (100 μm) were cut with a Microm cryostat and mounted on superfrost plus glass slides (Fisher Scientific, Pittsburgh, PA). Frozen slides were instantly exposed the film in an imaging cassette BAS- TR2025 (Fuji Photo Film, Tokyo, Japan) for 12 h in −20° C at dark. The distribution of radioactivity was visualized by a Fuji Bio-Imaging Analyzer FLA-7000 (Fuji Photo Film, Tokyo, Japan). Photo-stimulated luminescence (PSL) from the striatum and cerebellum were quantified using Multi Gauge v3.0 software (Fuji Photo Film, Tokyo, Japan). Data were background-corrected and expressed as photo-stimulated luminescence signals per square millimeter (PSL/mm²).

Liu H., Jin H., Yue X., Zhang X., Yang H., Li J., Flores H., Su Y., Perlmutter J.S, & Tu Z. (2015). Preclinical evaluation of a promising C-11 labeled PET tracer for imaging phosphodiesterase10A in the brain of living subject. NeuroImage, 121, 253-262.

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In Vivo Imaging of TZ659 Binding in Rat Brain

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Adult male SD rats (250-300 g, n = 3) were injected with 4.0 - 4.5 mCi (-)-[¹¹C]TZ659. Rats were euthanized 60 min after injection, and the whole brain was immediately removed. Intact brains were snap frozen over dry ice. Horizontal sections (100 μm) were cut with a Microm cryotome and mounted on Superfrost Plus glass slides (Fisher Scientific, Pittsburgh, PA). Frozen slides were directly exposed to film in an imaging cassette BAS-TR2025 (Fuji Photo Film Co., Tokyo, Japan) for 12 hours at -80 °C in the dark. The distribution of radioactivity was visualized by a Fuji Bio-Imaging Analyzer FLA-7000 (Fuji Photo Film Co., Tokyo, Japan). Photo-stimulated luminescence (PSL) from the striatum and cerebellum was quantified using Multi Gauge v3.0 software (Fuji Photo Film Co., Tokyo, Japan). Data were background-corrected and expressed as photo-stimulated luminescence signals per square millimeter (PSL/mm²).

Liu H., Jin H., Li J., Zhang X., Kaneshige K., Parsons S.M., Perlmutter J.S, & Tu Z. (2015). In vitro and ex vivo characterization of (-)-TZ659 as a ligand for imaging the vesicular acetylcholine transporter. European journal of pharmacology, 752, 18-25.

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Validating Tracer Uptake in Spinal Cord

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To exclude the possibility that the increase of tracer uptake was caused by the leakage of blood-brain barrier (BBB), 20 μm slices of snap-frozen rat lumbar spinal cord were incubated with 1 nM [¹⁸F]VAT in the assay buffer (50 mM Tris-HCl, 120 mM NaCl, 5 mM KCl, 2 mM CaCl₂, 1 mM MgCl₂, pH 7.4) at room temperature for 60 min. Non-specific binding was determined by using adjacent sections incubated with an additional 10 μM vesamicol, a potent VAChT Inhibitor, in the assay buffer. Following the incubation, the sections were washed and exposed to the storage phosphor screen in an imaging cassette overnight in −80 °C in the dark. The distribution of radioactivity were visualized by a Fuji Bio-Imaging Analyzer FLA-7000 (Fuji Photo Film, Tokyo, Japan).

Liu C., Liu H., Jin H., Yue X., Luo Z, & Tu Z. (2018). Cholinergic imbalance in lumbar spinal cord of a rat model of multiple sclerosis. Journal of neuroimmunology, 318, 29-35.

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Ex vivo Autoradiography of Carotid Injury

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Ex vivo autoradiography was performed at ∼72 hours following the carotid injury. Rats were euthanized at 30 minutes following a bolus injection of [¹¹C]TZ3321 (74-110 MBq). Bilateral common carotid arteries were then dissected and cut in half. One half of the artery was fixed in 10% formalin and processed for histological and immunohistochemical examination. The other half was exposed immediately to a BAS storage phosphor screen (BAS-IP-MS-2025) in an imaging cassette for 2 hours at −80°C. The distribution of radioactivity was visualized by a Fuji Bio-Imaging Analyzer FLA-7000 (Fuji Film, Tokyo, Japan). Photostimulated luminescence was quantified using Multi Gauge v3.0 software. Data were background corrected and expressed as photostimulated luminescence signals per square millimeter (PSL/mm²).

Liu H., Jin H., Yue X., Han J., Baum P., Abendschein D.R, & Tu Z. (2017). PET Study of Sphingosine-1-Phosphate Receptor 1 Expression in Response to Vascular Inflammation in a Rat Model of Carotid Injury. Molecular imaging, 16, 1536012116689770.

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