Intracellular edema of retinal Müller cell in vivo was evaluated according to the published method.21 Briefly, the rats were killed, the eyes were enucleated and fixed in 2.5% glutaraldehyde for 30 min. The eyes were dissected under a dissecting microscope and the anterior parts of the eye were removed. The posterior part was fixed in 2.5% glutaraldehyde for 5 more hours, which were then dehydrated in a graded alcohol series (50, 70, 95, and 100%) and embedded in epoxy resin for sectioning. During the sample preparation of semithin section, the retinas were cut into several small pieces about 2.25 mm2. Semithin sections (1 μm) were cut using an ultramicrotome (EM UC7, Leica, Germany) and stained with toluidine blue. The morphology of swollen Müller cell was examined under a light microscope (Tecnai G2 20 TWIN, FEI, USA). For quantitation, the Müller intracellular edema was calculated as the number of ribbon-like gaps per 200 μm of the retina.
G2 20 twin
Manufactured by Thermo Fisher Scientific
Sourced in United States
The G2 20 TWIN is a laboratory instrument designed for quantitative analysis. It features dual mass spectrometry detection capabilities.
Automatically generated - may contain errors
Lab products found in correlation
Em uc7, by Leica (1 mentions)
Evo 18 sem, by Zeiss (1 mentions)
Escalab 250, by Thermo Fisher Scientific (1 mentions)
S 4800, by Hitachi (1 mentions)
Emfc 6 ultramicrotome device, by Leica (1 mentions)
Ultracut ultramicrotome, by Leica (1 mentions)
G1102, by Wuhan Servicebio Technology (1 mentions)
Uc7 ultramicrotome, by Leica (1 mentions)
Ultrascan 4000, by Ametek (1 mentions)
Vitrobot mark 4, by Thermo Fisher Scientific (1 mentions)
D8 advance, by Bruker (1 mentions)
20 protocols using g2 20 twin
1
Evaluation of Retinal Müller Cell Edema
2
Morphological Characterization of Plasma-Treated Nanoparticles
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Physical changes in the virgin-NPs interacted with HSA and plasma was examined under HR-TEM (Technai, G2 20 Twin, FEI, USA) as well as HR-SEM (Carl Zeiss Evo 18 SEM, Germany) by fixing a thin film of HSA/plasma-NP complex on copper grid. Prior to SEM analysis, the sample was coated with gold using sputter coater.
3
Ultrastructural Analysis of Cotton Fiber Development
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Electron microscopic analyses were conducted essentially as previously described (Pugh et al. 2010 ). Wild‐type cotton ovules harvested at 1 DPA were cultured for 7 d with either 1 μM GA3, 5 μM C26:0 or 2.2 ppm ethylene added to the media. Cotton fibers from various treatments were fixed for 12 h in 2.5% glutaraldehyde. After three washes with distilled and deionized water, the fibers were incubated in 1.5% osmium tetraoxide for 4 h at room temperature. Samples were dehydrated in a series of ethanol (50%–100%) at 30 min intervals and epoxy ethane and then embedded in Epon resin for 48 h at 60 °C. Fibers at the base of the ovule were cut into 50 nm thin sections, stained with uranyl acetate and lead citrate, then photographed under a transmission electron microscope (Tecnai G2 20 TWIN, FEI).
4
Characterization of nzf-GO Nanocomposites
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All the nzf-GO scaffold nanocomposites were characterized in a transmission electron microscope (TEM) (TECNAI G2 20 TWIN, FEI, USA) where the as-synthesized nanocomposite samples were dispersed in solvent and drop cast on a lacy carbon coated TEM grid.
5
Ultrastructural Analysis of Hepatocyte Responses
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GFP+ primary mouse hepatocytes treated with DMSO (Veh), GW7647 (5μM), or GW4064 (10μM) for 24h to transmission electron microscopic. For transmission electron microscopic (TEM) observations, the samples were fixed in 4% paraformaldehyde and 2.5% glutaraldehyde in 0.1M phosphate buffer for overnight. After washing in 0.1M phosphate buffer, the samples were postfixed with 1% osmium tetroxide in same buffer for 1h. Then the samples were dehydrated with a series of the graded ethyl alcohol. The samples were embedded in Epon 812 and then polymerization was performed at 60°Cfor 3 days. Ultrathin sections (60∼70nm) were obtained by ultramicrotome (Leica UC7, Germany). Images were acquired with transmission electron microscope (FEI, TECNAI G2 20 TWIN, USA) after double staining with uranium acetate and lead citrate.
6
Autophagosome Quantification in NP Cells
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NP cells were fixed in 2.5% glutaraldehyde overnight, post-fixed in 2% osmium tetroxide for 1 h and stained with 2% uranyl acetate for 1 h. After dehydration in an ascending series of acetone, the samples were embedded into Araldite. Samples were cut into ultrathin sections, and then stained with toluidine blue. Finally, sections were observed using a transmission electron microscope (TEM) (Tecnai G2 20 TWIN, FEI, United States). Randomized fields were captured and the autophagosomes in the field were counted.
7
Comprehensive Characterization of MnO2 Powder
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An X-ray diffractometer (XRD)
was used to identify the phase of the prepared MnO2 powder
using the D8 Advance Bruker instrument using monochromatic Cu Kα
radiation (λ = 1.5406 Å). X-ray Rietveld refinement was
carried out with the FullProf program using the pseudo-Voigt profile
function. Raman studies were performed with a LabRAM HR, 532 nm laser
excitation. X-ray photoelectron spectroscopy (XPS) analysis was carried
out for the samples, using an ESCALAB 250 (ThermoElectron, Al Kα)
spectrometer. The electron microscopy analysis was carried out using
a field emission scanning electron microscope (Hitachi S-4800) and
a high-resolution transmission electron microscope (TECNAI G2 20 Twin,
FEI). The details about electrochemical analysis and first-principles
calculations are as perNote S2 .
was used to identify the phase of the prepared MnO2 powder
using the D8 Advance Bruker instrument using monochromatic Cu Kα
radiation (λ = 1.5406 Å). X-ray Rietveld refinement was
carried out with the FullProf program using the pseudo-Voigt profile
function. Raman studies were performed with a LabRAM HR, 532 nm laser
excitation. X-ray photoelectron spectroscopy (XPS) analysis was carried
out for the samples, using an ESCALAB 250 (ThermoElectron, Al Kα)
spectrometer. The electron microscopy analysis was carried out using
a field emission scanning electron microscope (Hitachi S-4800) and
a high-resolution transmission electron microscope (TECNAI G2 20 Twin,
FEI). The details about electrochemical analysis and first-principles
calculations are as per
8
Ultrastructural Analysis of MGC803 Cells
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MGC803 cells in 6-well plates were fixed in 2.5% glutaraldehyde for 2 h, and then dehydrated in a graded ethanol series and embedded. Ultrathin sections were mounted and post-stained with 2% uranyl acetate followed by 0.3% lead citrate. Sections were imaged using a transmission electron microscope G2 20 Twin (FEI, USA).
9
TEM Analysis of Nanocomposite Morphology
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The nanocomposites
morphology was observed by transmission electron microscopy (TEM)
with a TECNAI G2 20 TWIN (FEI) microscope, operating at an accelerating
voltage of 200 kV in bright-field mode. Samples were sectioned with
a Leica EMFC 6 ultramicrotome device at −25 °C equipped
with a diamond knife. The 300 mesh copper grids were used to support
the ultrathin sections (∼100 nm).
morphology was observed by transmission electron microscopy (TEM)
with a TECNAI G2 20 TWIN (FEI) microscope, operating at an accelerating
voltage of 200 kV in bright-field mode. Samples were sectioned with
a Leica EMFC 6 ultramicrotome device at −25 °C equipped
with a diamond knife. The 300 mesh copper grids were used to support
the ultrathin sections (∼100 nm).
10
Ultrastructural Analysis of Mouse Hippocampal Synapses
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According to a published protocol [23 (link)], after anesthesia, mice were injected into the left ventricle with 4% paraformaldehyde. Hippocampal sections were fixed in cold 1% OsO4 for 1 h. The specimens were prepared and tested according to standard procedures. Ultrathin Sects. (90 nm) were stained with uranyl acetate and lead acetate and observed under a transmission electron microscope (Tecnai G2 20 Twin, FEI, USA). Synapses were identified based on synaptic vesicles and postsynaptic density.
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