Recugel

Spectral domain optical coherence tomography (SD-OCT) images were obtained using the Bioptigen Spectral Domain Ophthalmic Imaging System (SDOIS; Envisu R2200, Bioptigen, Morrisville, NC, USA). ALMS mice were anesthetized and pupils were dilated by applying 1–2 drops of topical 0.5% tropicamide (Visufarma, Rome, Italy). To prevent corneal desiccation during the procedure, topical lubricant eye drops (Recugel; Bausch & Lomb, Rochester, NY, USA) were applied bilaterally with a small brush. Mice were positioned into the animal imaging mount and rodent alignment stage (AIM-RAS; Bioptigen, Morrisville, NC, USA); the laser source was placed in front of the mouse, and images were acquired by the InVivoVue Clinic software (Bioptigen, Morrisville, NC, USA). Three images, one central, one superior, and one inferior to the optic nerve, were taken from each eye. ONL thickness was manually measured three times from each OCT scan image and averaged.

Spectral domain optical coherence tomogram images were obtained using the Bioptigen Spectral Domain Ophthalmic Imaging System (SDOIS; Envisu R2200; Bioptigen, Inc., Morrisville, NC). Mice were anesthetized, and pupils were dilated by applying one or two drops of topical 0.5% tropicamide (Visufarma, Rome, Italy). To prevent corneal desiccation during the procedure, topical lubricant eye drops (Recugel; Bausch & Lomb, Rochester, NY) were applied bilaterally with a small brush. Mice were positioned into the animal imaging mount and rodent alignment stage (AIM-RAS; Bioptigen, Inc.). The laser source was placed in front of the mouse, and images were acquired by the InVivoVue Clinic software (Bioptigen, Inc.). Three images—one central, one superior, and one inferior to the optic nerve—were taken from each eye. Outer nuclear layer thickness was manually measured three times from each OCT scan image and averaged.