Bond elut

Manufactured by Agilent Technologies

Sourced in United States

Bond Elut is a solid-phase extraction (SPE) product line offered by Agilent Technologies. It is designed for sample preparation and analyte extraction in various analytical applications.

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Lab products found in correlation

Mc rr, by Enzo Life Sciences (2 mentions) Mc lr, by Enzo Life Sciences (2 mentions) Mc yr, by Enzo Life Sciences (2 mentions) Pepsin, by Merck Group (2 mentions) Eclipse xdb c18, by Agilent Technologies (1 mentions) Cylindrospermopsin standard, by Enzo Life Sciences (1 mentions) Milli q water purification system, by Merck Group (1 mentions) Rtx 225, by Restek (1 mentions) Qp2010, by Restek (1 mentions) Seppack, by Waters Corporation (1 mentions) Millex gv, by Merck Group (1 mentions) Zearalatest column, by VICAM (1 mentions) Whatman no 5 paper, by Cytiva (1 mentions) Whatman no 4 filter paper, by Cytiva (1 mentions) Sodium sulphate, by Merck Group (1 mentions) Chloride acid, by Merck Group (1 mentions) Sodium dihydrogen phosphate, by Merck Group (1 mentions) Acetonitrile, by Avantor (1 mentions) Mucin, by Merck Group (1 mentions) Bile salt, by Merck Group (1 mentions)

35 protocols using bond elut

Organic Acid Extraction and HPLC Analysis

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Hemolymph was centrifuged (120 g/5 min, 2 °C) to separate the serum from the hemocytes and tissue particles. From the serum, organic acids were extracted by an ion exchange chromatographic column (Bond Elut  -Varian). Using a vacuum pump, this column was activated by 1 mL of HCL (0.5 mol/L), 1 mL methanol and 2 mL of ultrapure water. Then, 250 μL hemolymph was applied to the column. After application of the sample, 2mL of ultrapure water was added, the Bond Elut was removed from vacuum, 250 μL 0.5 M H 2 SO 4 was added, and the mixture was centrifuged at 500g/5minutes, 2 °C (Bezerra et al., 1999) (link).
The resulting sample was subjected to high performance liquid chromatography (HPLC -Varian ProStar) with an exclusion column (BIORAD-Aminex ion exclusion HPX -87H, 300 × 7.8 mm). A pre-column (BIORAD-Aminex HPX -85) protects the separation column. The eluent used in the mobile phase was the sulfuric acid (5 mmol/L) at room temperature with 0.6 mL/minute flow, attached to an UV-visible light detector at a wavelength of 210 nm. Each sample injected contained 50 μL volume.
The retention time of the organic acids and the peak area of the substances were calculated by ProStar Varian  software providing their concentrations in the sample. Organic acids were identified accordingly to their retention time and to the calibration previously performed in HPLC (Bezerra et al., 1999) (link).

Silva L.D., Amaral V.C.S., Vinaud M.C., Castro A.M., Rezende H.H.A., Santos D.B., Mello-Silva C.C, & Bezerra J.C.B. (2017). Changes in energetic metabolism of Biomphalaria glabrata (Mollusca, Planorbidae) in response to exogenous calcium. Brazilian journal of biology = Revista brasleira de biologia, 77(2).

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Molecular Characterization of DOM via FT-ICR MS

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DOM in stream water samples was isolated for FT-ICR MS analysis via
solid-phase extraction (SPE) with 100mg Bond Elut (Agilent Technologies)
styrene-divinylbenzene copolymer (PPL) columns. Depending on a sample’s
DOC concentration, the volume used for extraction was adjusted such that 50
µg of C was eluted with 1ml of HPLC-grade methanol into a pre-combusted
glass vial. The molecular composition of PPL-extracted DOM was determined using
the 21-Tesla FT-ICR MS at the National High Magnetic Field Laboratory (NHMFL) at
Florida State University, Tallahassee, FL, USA^{64 (link),65 (link)}. Negative ions were
generated by injecting the extracted sample into the mass spectrometer via
negative electrospray ionization (negative ESI) at a flow rate of 500 nL
min^-1. Mass spectra were generated as the sum of 100 individual
spectra scans for each sample. See supporting text for formula assignment methods.

Drake T.W., Van Oost K., Barthel M., Bauters M., Hoyt A.M., Podgorski D.C., Six J., Boeckx P., Trumbore S.E., Ntaboba L.C, & Spencer R.G. (2019). Mobilization of aged and biolabile soil carbon by tropical deforestation. Nature geoscience, 12(7), 541-546.

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Quantification of Peramine in Plant Extracts

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The 100-mg freeze-dried shoot powder was extracted in a solution of 3 ml of methanol and 3 ml of trichloromethane for 30 min under ultrasonic cleaner. The mixture material was centrifuged for 10 min at 1000 rpm, where 3 ml of n-hexane and 3 ml of ultra-pure water were added respectively, then extracted for 30 min and centrifuged for 12 min at 1000 rpm. Peramine was removed from shoot powder extracts by passing 1-ml portions through preconditioned 1-ml Agilent Bond Elut carboxylic acid (CBA) columns packed with 100 mg of adsorbent. The peramine was then eluted with 1 ml of a 5% formic acid-40% methanol solution. Peramine was measured by HPLC with an Agilent (Agilent 1100, America) liquid chromatograph fitted with a C18 column (Eclipse XDB-C18, 250 mm × 4.6 mm, 5 μm). Detection was performed with an ultraviolet (UV) wavelength spectrophotometric detector set at 280 nm. Mobile phase “A” was 1.8 g L⁻¹ guanidine carbonate, and phase “B” was acetonitrile. The quantity of peramine in 25-μl injection samples was determined, based on pre-established standard curve peak area values. All reagents were chromatographically pure.

Lin W., Kuang Y., Wang J., Duan D., Xu W., Tian P., Nzabanita C., Wang M., Li M, & Ma B. (2019). Effects of Seasonal Variation on the Alkaloids of Different Ecotypes of Epichloë Endophyte-Festuca sinensis Associations. Frontiers in Microbiology, 10, 1695.

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Flazin Extraction and Quantification in Foods

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Flazin in foods was isolated by solid phase extraction (SPE) with
propylsulfonicacid-derivatized silica PRS cartridges (Bond Elut, 500
mg, 3 mL volume, Agilent). For that, samples of solid foods (2–5
g) or liquid foods (5 mL) were added to 0.6 M HClO₄ (15–20
mL), homogenized with an Ultraturrax homogenizer, and centrifuged
(12,000g, 5 °C) for 15 min. PRS cartridges were
conditioned with 6 mL of methanol followed by 6 mL of 0.1 M HCl. Sample
aliquots (5 mL) were spiked with 0.5 mL of 1-ethyl-β-carboline
solution (EβC) (0.08 mg/L) as internal standard (IS) and loaded
onto the PRS cartridges in a vacuum manifold. After washing with deionized
water (2 mL), flazin was eluted with 3 mL of 0.4 M K₂HPO₄ (adjusted to pH 9.1), followed by 3 mL of 0.4 M K₂HPO₄ (pH 9.1)/methanol (1:1). These two phosphate fractions
were mixed and subsequently analyzed by HPLC-fluorescence and HPLC-MS.
The SPE procedure gave recoveries of flazin (80 μg/L) higher
than 95% (n = 3) with a repeatability of 2.6% RSD
(n = 3) and an accuracy of 8% mean error (n = 3).

Herraiz T, & Salgado A. (2024). Formation, Identification, and Occurrence of the Furan-Containing β-Carboline Flazin Derived from l-Tryptophan and Carbohydrates. Journal of Agricultural and Food Chemistry, 72(12), 6575-6584.

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Coastal Seawater and River Water Extraction

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Surface coastal seawater
was collected off the Ellen Browning Scripps Memorial Pier (32°52′01.5″N
117°15′26.9″W) on February 26, 2021 between 11:00
and 19:00 PDT. Seawater was stored in acid-washed 20-L HDPE carboys
(Nalgene), filtered through an AcroPak 0.8/0.45 μm Supor membrane
filter (Pall Corporation), and subsequently acidified to pH ∼
2 (37% HCl, TraceMetal Grade, Fischer Chemical). The seawater was
extracted through PPL cartridges (Bond Elut, Agilent).^{53 (link)} River water samples were collected from the
Neckar river in Tübingen, Germany, in the morning of February
16, 2022. The water was collected in glass bottles, previously washed
with 100% methanol, via grab sampling. Samples were immediately brought
to the laboratory for processing and analysis. Half of the sample
volume was acidified to pH ∼ 2 (37% HCl, TraceMetal Grade,
Fischer Chemical). For solid phase extraction of the nonacidified
river water, and acidified river water three different types of resins
were used: C18, PPL, and HLB. Extracts were pooled together for the
analysis to a final concentration of 10 mg/mL in 50% MeOH.

Stincone P., Pakkir Shah A.K., Schmid R., Graves L.G., Lambidis S.P., Torres R.R., Xia S.N., Minda V., Aron A.T., Wang M., Hughes C.C, & Petras D. (2023). Evaluation of Data-Dependent MS/MS Acquisition Parameters for Non-Targeted Metabolomics and Molecular Networking of Environmental Samples: Focus on the Q Exactive Platform. Analytical Chemistry, 95(34), 12673-12682.

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SPE-Based Compound Extraction

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The SPE clean-up protocol used for sample extracts was based on [18 (link)]. Briefly, a 1 g amino cartridge (Bond Elut, Agilent, California) was conditioned with 6 mL of chloroform. Sample residues were re-dissolved in chloroform and loaded onto SPE cartridges. The cartridges were then washed with an additional 6 mL of chloroform, and eluted with chloroform:isopropanol (2:1). Eluants were collected and dried at 65°C. Final residues were redissolved in 500 μL methanol before LC-MS/MS analysis.

Hardison D.R., Holland W.C., McCall J.R., Bourdelais A.J., Baden D.G., Darius H.T., Chinain M., Tester P.A., Shea D., Flores Quintana H.A., Morris JA J.r, & Litaker R.W. (2016). Fluorescent Receptor Binding Assay for Detecting Ciguatoxins in Fish. PLoS ONE, 11(4), e0153348.

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Determination of Cyanotoxins in Lettuce Samples

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Three congeners of MCs (MC-LR, MC-RR, and MC-YR) (99% purity) and Cylindrospermopsin standard (95% purity) were purchased from Enzo Life Sciences (Lausen, Switzerland). Deionized water (18.2 MΩ cm resistivity) was obtained from a Milli-Q water purification system (Millipore, Bedford, MA, USA). HPLC-grade methanol, dichloromethane (DCM), formic acid (FA), acetonitrile, and sodium hydroxide (NaOH) were supplied by Merck (Darmstadt, Germany). For the SPE, C18 cartridges were Bakerbond^® (500 mg, 6 mL), purchased from Dicsa (Andalucía, España), and graphitized carbon cartridges were BOND ELUT^® (500 mg, 6 mL), supplied by Agilent Technologies (Amstelveen, The Netherlands). For UHPLC–MS/MS analyses, reagents were of LC–MS grade: Water and acetonitrile were supplied by VWR International (Fontenay-sous-Bois, France) and formic acid by Fluka (Steinheim, Germany). A standard multitoxin solution containing 100 µg L⁻¹ of each cyanotoxin (MC-LR, MC-RR, MC-YR, and CYN) was prepared in 20% MeOH to be further diluted to three different concentrations (5, 20, and 50 µg L⁻¹), used as working solutions. Lettuce control samples (without toxins) were obtained from a local supermarket, ready for human consumption.

Díez-Quijada L., Guzmán-Guillén R., Prieto Ortega A.I., Llana-Ruíz-Cabello M., Campos A., Vasconcelos V., Jos Á, & Cameán A.M. (2018). New Method for Simultaneous Determination of Microcystins and Cylindrospermopsin in Vegetable Matrices by SPE-UPLC-MS/MS. Toxins, 10(10), 406.

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Folch Lipid Extraction and Fractionation

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Total lipids from cells were extracted using a modified Folch extraction method as described,³⁷ dried under nitrogen stream, and stored at −20°C for subsequent processing. Neutral lipid fraction was isolated in chloroform using solid‐phase extraction columns (Bond Elut, 12113014, Agilent).

Petkevicius K., Bidault G., Virtue S., Jenkins B., van Dierendonck X.A., Dugourd A., Saez‐Rodriguez J., Stienstra R., Koulman A, & Vidal‐Puig A. (2021). Norepinephrine promotes triglyceride storage in macrophages via beta2‐adrenergic receptor activation. The FASEB Journal, 35(2), e21266.

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Quantification of Kidney Angiotensin Peptides

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For whole kidney tissue Ang II and Ang III measurements, kidneys were immersed in cold methanol (100%), minced, and homogenized with a polytron tissue tearer. The homogenates were centrifuged, and the supernatants from the kidney homogenates were dried overnight in a vacuum centrifuge. The dried residue was reconstituted in 1 mL buffer as described in the Enzyme Immunoassay (EIA) Kit (Phoenix Pharmaceutical, Inc). These samples were applied to phenyl‐bonded solid‐phase extraction columns (Bond Elut; Agilent Technologies, Inc) that had been prewashed with 90% methanol followed by water. After sample application, each solid‐phase extraction column was washed sequentially with water, hexane, and chloroform. Ang peptides were eluted from the solid‐phase extraction column with 90% methanol.17 The eluates were collected, evaporated to dryness under vacuum, and stored at −20°C.

Kemp B.A., Howell N.L., Keller S.R., Gildea J.J., Shao W., Navar L.G, & Carey R.M. (2019). Defective Renal Angiotensin III and AT2 Receptor Signaling in Prehypertensive Spontaneously Hypertensive Rats. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 8(9), e012016.

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Quantifying Urinary PAH Metabolites

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Levels of 1-OHP, 2-OHF, 1-NAPH and 2-NAPH in urine were measured after solid phase extraction (SPE) using liquid chromatography with tandem mass spectrometry (LC-MS-MS). In brief, 1 ml of urine was mixed with a buffer solution and deconjugated (β-glucoronidase, 37 °C, 18–20 h), after which deuterium-labeled internal standards were added. The samples were loaded onto pre-washed 500 mg C18 SPE-columns (Bond Elut, Agilent Technologies) and subsequently washed using 6 ml methanol:water (1:3) and 6 ml water. The SPE-columns were dried overnight at 55 °C and eluted with 3 ml methanol. The extract were evaporated to dryness under a gentle stream of nitrogen and reconstituted in 300 μL methanol. The extracts were analyzed on an Agilent LC-MS-MS (series 6460) using a Phenomenex C18, 100 Å, 100 × 2 mm column with a gradient of water and methanol. 1-OHP and 2-OHF were quantified using d₉–1-OHP while d₈–2-NAPH was used for 1-NAPH and 2-NAPH. The limit of quantification was set to 10 times the signal-to-noise ratio.
All urine concentrations were standardized for diuresis with the concentration of creatinine as previously described [61 (link)].

Andersen M.H., Frederiksen M., Saber A.T., Wils R.S., Fonseca A.S., Koponen I.K., Johannesson S., Roursgaard M., Loft S., Møller P, & Vogel U. (2019). Health effects of exposure to diesel exhaust in diesel-powered trains. Particle and Fibre Toxicology, 16, 21.

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