For assessment of 5-HT and 5-HIAA levels in wild-type (WT) and Pcdh15av-3J mice, tissues were homogenized using an Omni Tissue Homogenizer (Omni International, Kennesaw, GA, USA) in 100–750 μl of 0.1 m trichloroacetic acid (TCA), 10 mm sodium acetate, 0.1 mm ethylenediaminetetraacetic acid (EDTA), 5 ng/ml isoproterenol (as internal standard) and 10.5% methanol (pH 3.8). 5-Hydroxytryptamine and 5-HIAA were determined by HPLC through the Neurochemistry Core of the Vanderbilt Brain Institute, utilizing an Antec Decade II (oxidation: 0.5) electrochemical detector (Antec LLC, Boston, MA, USA) operated at 33°C. Twenty microliter samples of the supernatant were injected using a Waters 717+ autosampler (Waters, Milford, MA, USA) onto a Phenomenex Nucleosil (5u, 100A) C18 HPLC Column (150 × 4.60 mm2) (Phenomenex, Torrance, CA, USA). Samples were eluted with a mobile phase consisting of 89.5% 0.1 m TCA, 10 mm sodium acetate, 0.1 mm EDTA and 10.5% methanol (pH 3.8). Solvent was delivered at 0.6 ml/min using a Waters 515 HPLC Pump. HPLC control and data acquisition were managed by Millennium 32 software.
Waters 717 autosampler
Manufactured by Waters Corporation
Sourced in United States
The Waters 717+ autosampler is a laboratory instrument designed for automated sample handling and injection. It is capable of accurately and precisely delivering liquid samples to an analytical instrument, such as a chromatograph, for analysis. The 717+ autosampler features programmable sample delivery and can accommodate a wide range of sample vial sizes and types.
Automatically generated - may contain errors
2 protocols using waters 717 autosampler
1
Quantifying 5-HT and 5-HIAA in Mice
2
Hydrogel-Based 5-FU Release Kinetics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
5-FU was selected as a hydrophilic model drug to study the in vitro drug release profile from the hydrogels. Firstly, 5-FU was encapsulated in hydrogels by dissolution in a dynamer solution, followed by gelation process after mixing with O-CMCS solution. Then the release profile was studied by immersing the hydrogel in 1 mL PBS buffer (pH 7.4) at 37°C. At each preset time interval, the whole medium was collected for measurements, and replaced by 1 mL fresh PBS solution. The released amount of 5-FU was determined by HPLC measurements.
High-performance liquid chromatography-UV (HPLC-UV) was performed by using a HPLC system equipped with Waters 717 Autosampler, Waters 616 Pump, Waters 2996 Photodiode Array Detector, and a reverse phase Thermo Scientific C18 column (L ¼ 250 mm, I.D ¼ 4.6 mm, and 5 mm particles). The mobile phase was a Buffer A (HPLC grade water 0.1% trifluoroacetic acid) and Buffer B (HPLC grade acetonitrile 0.1% trifluoroacetic acid). The flow rate was 1 mL min−1.
High-performance liquid chromatography-UV (HPLC-UV) was performed by using a HPLC system equipped with Waters 717 Autosampler, Waters 616 Pump, Waters 2996 Photodiode Array Detector, and a reverse phase Thermo Scientific C18 column (L ¼ 250 mm, I.D ¼ 4.6 mm, and 5 mm particles). The mobile phase was a Buffer A (HPLC grade water 0.1% trifluoroacetic acid) and Buffer B (HPLC grade acetonitrile 0.1% trifluoroacetic acid). The flow rate was 1 mL min−1.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!