Anti gata 3 twaj

Manufactured by Thermo Fisher Scientific

The Anti-GATA-3 (TWAJ) is a laboratory reagent designed for research purposes. It is an antibody that specifically binds to the GATA-3 protein, a transcription factor involved in various biological processes. This product can be used in various research applications, such as cell identification and characterization, without making claims about its intended use.

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Lab products found in correlation

Anti foxp3 fjk 16s, by Thermo Fisher Scientific (3 mentions) Anti ubiquitin, by Merck Group (2 mentions) Carboxyfluorescein succinimidyl ester (cfse), by Thermo Fisher Scientific (2 mentions) Anti human cd8 rpa t8, by BD (2 mentions) Anti t bet ebio4b10, by Thermo Fisher Scientific (2 mentions) Total nedd4 2, by Cell Signaling Technology (2 mentions) Tcf 1 2206, by Cell Signaling Technology (2 mentions) To phosphorylated stat6, by Cell Signaling Technology (2 mentions) Anti t bet 4b10, by Thermo Fisher Scientific (2 mentions) Anti junb 210, by Santa Cruz Biotechnology (2 mentions) Mg132, by Merck Group (2 mentions) Actin, by Merck Group (2 mentions) Anti il 13 ebio13a, by Thermo Fisher Scientific (1 mentions) Anti siglecf e50 2440, by BD (1 mentions) Anti cd11b m1 70, by BioLegend (1 mentions) Anti cd19 6d5, by BioLegend (1 mentions) Anti nk1.1 pk136, by BioLegend (1 mentions) Anti cd3 17a2, by BioLegend (1 mentions) Anti cd45 30 f11, by BD (1 mentions) Anti cd45.2 104, by BioLegend (1 mentions)

3 protocols using anti gata 3 twaj

Immunological Profiling with Flow Cytometry

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The following antibodies for flow cytometry were from BD Biosciences: anti-B220 (RA3-6B2), anti-CD3 (2C11), anti-CD4 (RM4–5), anti-CD8 (Ly-3), anti-CD25 (PC61), anti-CD44 (IM7), anti-CD124 (mIL4R-M1), anti-IFN-γ (XMG1.2), anti-IL-17a (TC11-18H10), and anti-human CD8 (RPA-T8). Anti-CD62L (MEL-14), anti-IL-13 (eBio13A), anti-IL-5 (TRFK5), anti-T-bet (eBio4B10) and anti-Foxp3 (FJK-16s) were from eBioscience.
The following antibodies for immunoblotting were from Cell Signaling Technologies: Antibody to Rictor (2140), NDRG1 phosphorylated at Thr346 (5482), total NDRG1 (9408), antibody to Akt phosphorylated at S473 (4060), pan-Akt (4685), NEDD4-2 phosphorylated at Ser342 (4080), total NEDD4-2 (4013), TCF-1 (2206), antibody to β-catenin (9562), antibody to phosphorylated β-catenin (9561), antibody to phosphorylated STAT6 (9361). Anti-T-bet (4B10) and anti-GATA-3 (TWAJ) were from eBioscience. Anti-JunB (210) was from Santa Cruz Biotechnology. Anti-ubiquitin was and actin were from Sigma. MG132 was from Sigma, PP242 was from Calbiochem, and CFSE was from Invitrogen.

Heikamp E.B., Patel C.H., Collins S., Waickman A., Oh M.H., Sun I.H., Illei P., Sharma A., Naray-Fejes-Toth A., Fejes-Toth G., Sen J., Horton M.R, & Powell J.D. (2014). The AGC kinase serum- and glucocorticoid-regulated kinase 1 (SGK1) regulates TH1 and TH2 differentiation downstream of mTORC2. Nature immunology, 15(5), 457-464.

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Comprehensive Immune Cell Profiling

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For cell sorting and flow cytometry analysis, cell suspension were prepared as described above, incubated with fixable viability dye and subsequently stained with following fluorophore-conjugated monoclonal antibodies: anti-CD45 (30-F11, BD Biosciences), anti-CD45.2 (104, Biolegend), anti-CD90.2 (thy1.2) (53–2.1, Biolegend), anti-CD3 (17A2, Biolegend), anti-CD4 (Biolegend, RM4–5), anti-CD8 (53–6.7, Biolegend), anti-CD11b (M1/70; Biolegend), anti-CD11c (N418, Biolegend), anti-Ly6C (HK1.4, Biolegend), anti-Ly6G (1A8, BD Biosciences), anti-Siglec-F (E50–2440, BD Bioscience), anti-mouse MHC Class II (I-A/I-E) (M5/114.15.2, eBioscience), anti-NK1.1 (PK136, Biolegend), anti-CD19 (6D5, Biolegend), anti-T1/ST2 (DJ8, MD Biosciences), anti-KLRG1 (2F1, Biolegend), anti-FoxP3 (FJK-16S, eBiosciences), anti-Ki-67 (16A8, Biolegend), anti-Gata3 (TWAJ, eBioscience), anti-IL13 (eBio13A, eBioscience), anti-IFNgR1 (XMG1.2, BD Bioscience).

Cautivo K.M., Matatia P.R., Lizama C.O., Mroz N.M., Dahlgren M.W., Yu X., Sbierski-Kind J., Taruselli M.T., Brooks J.F., Wade-Vallance A., Caryotakis S.E., Chang A.A., Liang H.E., Zikherman J., Locksley R.M, & Molofsky A.B. (2022). Interferon gamma constrains type 2 lymphocyte niche boundaries during mixed inflammation. Immunity, 55(2), 254-271.e7.

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Immunological Profiling with Flow Cytometry

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