Sulphuric acid

Manufactured by Loba Chemie

Sourced in India, United Kingdom

Sulphuric acid is a colorless, dense, and highly corrosive liquid chemical. It is a strong mineral acid that is widely used in various industrial and laboratory applications.

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Lab products found in correlation

Sodium hydroxide (naoh), by Loba Chemie (5 mentions) Hydrochloric acid (hcl), by Loba Chemie (5 mentions) Methanol, by Loba Chemie (3 mentions) Ferric chloride, by Loba Chemie (2 mentions) Hexane, by Loba Chemie (2 mentions) Chloroform, by Loba Chemie (2 mentions) Sodium chloride (nacl), by Loba Chemie (2 mentions) Acetone, by Loba Chemie (2 mentions) Acetic acid, by Loba Chemie (1 mentions) Potassium permanganate, by Loba Chemie (1 mentions) Hydrogen peroxide, by Loba Chemie (1 mentions) Potassium nitrate, by Loba Chemie (1 mentions) Graphitepowder, by Alpha Chemika (1 mentions) Eosin y, by Daejung Chemicals (1 mentions) Sodium borohydride, by Merck Group (1 mentions) Luria bertani agar, by HiMedia (1 mentions) Acetonitrile, by Loba Chemie (1 mentions) Potato dextrose agar (pda), by Merck Group (1 mentions) Streptomycin, by Merck Group (1 mentions) L glutamine, by Merck Group (1 mentions)

11 protocols using sulphuric acid

Organic Solvents Extraction Protocol

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The chemicals and reagents that were used in this study includes, Analytical Reagent grade solvents, Petroleum ether, (Blulux Laboratories Pvt.Ltd, India), Ethyl Acetate 95 % (Loba Chemie Pvt.Ltd., India), methanol 99 % (Sisco Research laboratories Pvt.Ltd., India) chloroform (Blulux laboratories Pvt.Ltd., India), Potassium Iodide (UniChem Chemical, India), Iodine (Samir Tech-Chem Pvt. Ltd., India), Sodium hydroxide (Loba Chemie Laboratory Reagents, Fine Chemicals, India.), Hydrochloric acid (Loba Chemie Laboratory Reagents, Fine Chemicals), Sulphuric acid, Ferric chloride and Acetic acid (Loba Chemie Pvt.
Ltd., India).

Ameja L.B., Ayana M.T., Derese Z.B., Tariku Y., Yihun F.A., Kassa M.T., & Bahiru L.A. (2022). Qualitative phytochemical exploration guided in vitro antibacterial activity studies on crude stem bark extract of stereospermum kunthainum (Bignoniaceae).

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Graphene Oxide Synthesis and Characterization

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Reagents include graphite powder (Alpha Chemika, India), sulphuric acid (Loba Chemie Pvt. Ltd., Mumbai, 98%), potassium nitrate (Loba Chemie Pvt. Ltd., Mumbai, 99%), potassium permanganate (Loba Chemie Pvt. Ltd., Mumbai, 99%), hydrogen peroxide (Loba Chemie Pvt. Ltd., Mumbai, India), HCl (Molychem, India), Orange (II) (Alfa Aesa, UK), Eosin Y (Dae-Jung Chemicals & Metals Co. Ltd., Korea), sodium borohydride (Sigma Aldrich, USA) and H₂O₂ (Loba Chemie, India). The stock solution was made in double distilled water. Luria bertani agar (Himedia, India) used for the antibacterial assay.

Mayani S.V., Bhatt S.P., Mayani V.J, & Sanghvi G. (2023). Development of sustainable strontium ferrite graphene nanocomposite for highly effective catalysis and antimicrobial activity. Scientific Reports, 13, 6678.

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Metoclopramide Assay Development

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Methylated spirit (JHD, China), Sodium hydroxide, Sulphuric acid (99%) and acetonitrile were obtained from Loba Chemie Pvt. Ltd., metoclopramide tablets USP (10 mg, TEVA UK Limited, Eastbourne). All other chemicals were of HPLC grade.

Amadi C.N, & Nwachukwu W.I. (2020). The effects of oral administration of Cola nitida on the pharmacokinetic profile of metoclopramide in rabbits. BMC Pharmacology & Toxicology, 21, 4.

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Antimicrobial and Anticancer Evaluation

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Analytical reagent grade chemicals, including ferric chloride anhydrous, sulphuric acid, nitric acid, and hydrochloric acid, were procured from Loba Chemie Private Limited, Mumbai, India. Gum acacia powder, Meuller Hinton’s Agar, potato dextrose agar, Dulbecco’s Modified Eagles Medium, L-glutamine, and streptomycin (sulfate salt) were purchased from Sigma Aldrich, St. Louis, MO, USA. Ciprofloxacin, nutrient agar, nutrient broth, and streptomycin were procured from Hi-Media Private Limited, Mumbai, India. Gram-positive and Gram-negative food pathogenic microbial strains, i.e., Staphylococcus aureus (MTCC 3160) and Escherichia coli (MTCC 443), were obtained from the Microbial Type Culture Collection (MTCC), Institute of Microbial Technology, Chandigarh, India. Human colon adenocarcinoma cells (Caco-2 cells) were collected from National Centre for Cell Science, Pune, Maharashtra, India. Plastic dishes, plates, and transwell were obtained from Corning (Corning, NY, USA). Cellular grade, triple distilled water and aqua-regia washed glassware (class “A” certified) were used during research work.

Chawla P., Najda A., Bains A., Nurzyńska-Wierdak R., Kaushik R, & Tosif M.M. (2021). Potential of Gum Arabic Functionalized Iron Hydroxide Nanoparticles Embedded Cellulose Paper for Packaging of Paneer. Nanomaterials, 11(5), 1308.

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Phytochemical Analysis of Hagenia abyssinica

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All the chemicals and reagents used in this study were of analytical grade and double distilled water was used throughout the experiment. Silver nitrate (AgNO₃, 99.8% was purchased from Himedia Laboratories Pvt. Ltd., Mumbai, India); Ferric chloride (FeCl₃, 99% from Sisco Research Laboratories (Mumbai, India); Muller-Hinton Agar (MHA, were from Merck chemical company), Chloroform (CHCl₃, 99.9% from Fisher Scientific UK Limited, UK), Sulphuric acid (H₂SO₄, 98%), Sodium hydroxide (NaOH, 98%), Ascorbic acid (C₆H₈O₆, 99.9%) were purchased from Loba Chemie Pvt. Ltd (Mumbai, India); Hydrochloric acid (HCl, 37% from Blulux laboratory reagent, India), DPPH (C₁₈H₁₂N₅O₆, 99% from Sigma-Aldrich Pty Ltd, Germany); and Hagenia abyssinica (Bruce) J.F. Gmel plant leaves.

Melkamu W.W, & Bitew L.T. (2021). Green synthesis of silver nanoparticles using Hagenia abyssinica (Bruce) J.F. Gmel plant leaf extract and their antibacterial and anti-oxidant activities. Heliyon, 7(11), e08459.

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Phytochemical Profiling of E. conferta Roxb. Seeds

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Seeds of E. conferta Roxb. were procured from local market of Manali, Himachal Pradesh, India during the month of September-October and authenticated at G.B. Pant National Institute of Himalayan Environment and Sustainable Development, Himachal Pradesh, India (GBPNIHESD/ SIC/358). Hexane, methanol, gelatin, sodium chloride (NaCl), wagner reagent, hager reagent, mayer reagent, dragndroff reagent, ferric chloride (FeCl 3 ), chloroform (CHCl 3 ), acetic anhydride, sulphuric acid (H 2 SO 4 ), sodium hydroxide (NaOH), hydrochloric acid (HCl), ammonia (NH 3 ), molisch reagent, benedict reagent, fehling reagent, ninhydrin reagent, biuret reagent, potassium hydroxide (KOH) were purchased from Loba Chemie Pvt. Ltd., Mumbai, India.

Gupta M., & Singh N. (2021). PRELIMINARY PHYTOCHEMICAL SCREENING OF ELAEAGNUS CONFERTA ROXB. SEEDS AND BIOLOGICAL EVALUATION. PLANT ARCHIVES, 21(Suppliment-1), 547-551.

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Phytochemical and Pharmacological Evaluation

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Chemicals, drugs, and reagents used in the study include: distilled water, chloroform, ethyl acetate and mercuric chloride (Bulex Laboratory, India), nitrofurazone ointment 0.2%, Ketamine (NEON Laboratories, India), sulphuric acid, n-butanol, methanol, hexane (LobaChemie, India), wool fat, hard paraffin, white soft paraffin, cetostearyl alcohol, indomethacin (Cadila, Ethiopia), Tween 80 (Uni-Chem, India), diethyl ether (BDH Laboratory Supplies, England) and carrageenan (Sigma Aldrich, Germany), acetic anhydride (Lot A13/45/67/A), ammonia and ferric chloride anhydrous (Sisco Research Laboratories, India), potassium iodide (Calibre Engineering, India), normal saline (Addis Pharmaceutical Factory, Ethiopia). Chemicals and reagents to be used were analytically graded.

Mengie T., Mequanente S., Nigussie D., Legesse B, & Makonnen E. (2021). Investigation of Wound Healing and Anti-Inflammatory Activities of Solvent Fractions of 80% Methanol Leaf Extract of Achyranthes aspera L. (Amaranthaceae) in Rats. Journal of Inflammation Research, 14, 1775-1787.

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Analytical-Grade Chemical Preparation

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Hydrochloric acid 35.4% (Loba Chemie, India), sodium hydroxide (Loba Chemie Pvt. Ltd.), ammonium acetate 96% (Loba Chemie, India), methanol 99.8% HPLC grade (Loba Chemie, India), ethanol 96% (Fine Chemicals, Ethiopia), sodium chloride 99.5% (Loba Chemie, India), chloroform (Loba Chemie, India), acetone (Loba Chemie, India), and sulphuric acid (Loba Chemie, India) were purchased from local markets. All chemicals used in this experiment were analytical grade chemicals.

Haile T.G., Sibhat G.G, & Molla F. (2020). Physicochemical Characterization of Grewia ferruginea Hochst. ex A. Rich Mucilage for Potential Use as a Pharmaceutical Excipient. BioMed Research International, 2020, 4094350.

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Chromium Determination Protocol

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All the chemicals used in the present study were of analytical grade. Potassium dichromate (K₂Cr₂O₇), hydrochloric acid (HCl), sulphuric acid (H₂SO₄), 1,5 diphenyl carbazide, sodium hydroxide (NaOH) and acetone were purchased from Loba Chemie (U.K) and used as received. All the stock aqueous solutions and working dilutions were prepared using deionized water (Milli-Q ultrapure water).

Kulkarni P.S., Watwe V.S., Hipparge A.J., Sayyad S.I., Sonawane R.A, & Kulkarni S.D. (2019). Valorization of Uncharred Dry Leaves of Ficus benjamina towards Cr (VI) removal from Water: Efficacy Influencing Factors and mechanism. Scientific Reports, 9, 19385.

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Synthesis and Characterization of 4-Aminopyridine

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Silver nitrate (99 % (w/w), 169.87 g/mol), (98 % (w/w) purified 4-Aminopyridine with a molar mass of (94.12 g/mol) were attained from S. D. Fine Chemical Limited (Mumbai, India). Ethanol (99.9 %), sulphuric acid (98% (v/v)) were acquired from Loba Chemie Pvt. Ltd (Mumbai, India). (98%, (w/w)) refined sodium hydroxide, was purchased from Hi-Media Laboratories Pvt. Ltd (Mumbai, India). All the chemicals used for the study were of reagent grade. Clinically isolated bacterial strains of the bacterial pathogens Escherichia coli (E. Coli) and Bacillus subtilis (B. Subtilis) were obtained from the Microbial Type Culture Collection and Gene Bank in Punjab, India.
Structure and physiochemical properties of 4-Aminopyridine shown in (Table 1).

Attri P., Garg S., Ratan J.K, & Giri A.S. (2023). Silver nanoparticles from Tabernaemontana divaricate leaf extract: mechanism of action and bio-application for photo degradation of 4-aminopyridine. Environmental science and pollution research international, 30(10).

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