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Tensor 37 ft nir spectrometer

Manufactured by Bruker
Sourced in Germany

The TENSOR 37 FT-NIR spectrometer is a Fourier Transform Near-Infrared (FT-NIR) spectrometer manufactured by Bruker. The core function of the TENSOR 37 is to measure and analyze the near-infrared spectrum of samples.

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3 protocols using tensor 37 ft nir spectrometer

1

Near-Infrared Spectroscopy of Tea Samples

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A TENSOR37 FT-NIR spectrometer (Bruker, Ettlingen, Germany) was employed for the research. Each tea sample was packed in a quartz cuvette and detected with a PbS detector. Average values of 64 scanning spectra ranging from 4000 cm−1 to 12000 cm−1 were considered as the raw NIR data. Since the resolution was set as 8 cm−1 and the scanning interval was set as 1.928 cm−1, there were in total 4148 data points in the raw spectrum for each tea sample. Thus, the size of the raw dataset (2014 and 2015) was 1447 samples × 4148 variables. All the measurements were carried out at a room temperature of 25 ± 1°C.
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2

FT-NIR Spectroscopy Transmission Analysis

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NIRS were acquired using a Brucker TENSOR 37 FT-NIR spectrometer (Bruker Optik, Ettlingen, Germany) over the wavenumber range of 12000 to 4000 cm−1. A cuvette of 2 mm thickness was applied for the transmission spectrum research with a resolution of 8 cm−1 by averaging over 32 scans. Temperature and relative humidity remained approximately 23 °C and 55%, respectively. A background spectrum was collected for water. Triplicate spectra were collected consecutively for each sample.
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3

HPLC and NIR Analysis of Biotransformation

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Analysis was carried out on an Agilent–1200 HPLC system (Agilent Technologies, Waldbronn, Germany) equipped with a quaternary pump (G1311C), on-line degasser (G1322A), auto-sampler (G1229A), thermo column compartment (G1316A) and photo-diode-detector (G4212A). Near-infrared diffuse reflectance spectra were acquired using a Bruker TENSOR 37 FT-NIR spectrometer (Bruker Optik, Ettlingen, Germany) with an InGaAs detector and an Integrating Sphere Module over the wavenumber range of 12,000–4000 cm−1.
The biotransformation process was carried out in a 15 L glass tank bioreactor (Applikon Biotechnology, Delft, the Netherlands). The GAD genetically engineered super producer E. coli strain was cultivated in a 150 L fermenter (Biotech–2002 Bioprocess Controller, Baoxing, Shanghai, China).
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