For transmission electron microscopy (TEM) analysis, 5 μL of sample solution was loaded onto a copper-grid coated carbon film. After the sample had adsorbed for 1 min, the grid was washed with a drop of purified water and then negatively stained with 1% uranyl acetate for 1 min. Excess stain solution was removed using a piece of filter paper, and the grid was air-dried. The sample was imaged at a focus between 0.8 and 1.5 μm using a JEM-1400 Plus transmission electron microscope (JEOL Ltd., Tokyo, Japan) equipped with a Lab6 gun and operating at 120 kV. Images were recorded using an UltraScan OneView CMOS camera (Gatan, Pleasanton, CA, USA).
Lab6 gun
Manufactured by Ametek
Sourced in Japan, United States
The Lab6 gun is a precision laboratory instrument designed for controlled application of test materials. It features adjustable spray patterns and flow rates to enable accurate and consistent sample deposition. The device is intended for use in controlled laboratory environments by trained personnel.
Automatically generated - may contain errors
2 protocols using lab6 gun
1
TEM Analysis of Nanoparticle Samples
2
Cryo-TEM Characterization of Nanostructures
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Cryo-TEM measurements were carried out on a Tecnai G2 Sphera 20 electron microscope (FEI Company, Hillsboro, OR, USA) equipped with a LaB6 gun and Gatan 626 cryo-specimen holder (Gatan, Pleasanton, CA, USA). The sample preparation and imaging details are given in the Supporting Information (SI) section SI.1. All cryo-TEM pictures were carefully inspected for possible artifacts such as radiation damage and ice crystals, and high-quality images were CTF-corrected and band-pass filtered to suppress ice thickness variations and noise below 1 nm detail size.
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