H. felis strain CS1 (ATCC 49179) was used for the mouse infections. This strain was originally purchased from the American Type Culture Collection (ATCC). H. felis were maintained in both solid and liquid medium. The solid medium was composed of Columbia agar (BD Biosciences) supplemented with laked blood (5%, Hardy Diagnostics) and Amphotericin B (1%; Mediatech, Inc.). The liquid medium was composed of brain heart infusion (BHI; BD Biosciences) supplemented with 10%, heat inactivated fetal bovine serum (FBS). Bacteria were grown at 37°C under microaerophilic conditions (5% O2, 10% CO2, 85% N2) as described in previous studies by the authors (41 (link),43 (link)). Bacteria maintained in solid media were passaged every 2-3 days. Prior to the infection of the mice, H. felis were grown in liquid medium for 48 h. Spiral bacteria were counted using a Petroff-Hausser chamber.
Laked blood
Manufactured by Hardy Diagnostics
Laked blood is a laboratory specimen that consists of red blood cells that have been ruptured, causing the release of their contents, including hemoglobin, into the surrounding fluid. This process is known as hemolysis. Laked blood is commonly used in various diagnostic tests and procedures to assess the presence and concentration of specific analytes within the blood.
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Lab products found in correlation
2 protocols using laked blood
1
Culturing and Enumerating H. felis
2
Culturing H. pylori Strains for LPS Extraction
Check if the same lab product or an alternative is used in the 5 most similar protocols
H. pylori strains Sydney strain 1 (SS1) [27 (link)] and G27 (a kind gift from Dr. Guillemin, University of Oregon) were used in this study. H. pylori were routinely maintained on solid medium, Columbia agar (Becton Dickson, MD) supplemented with 5% laked blood (Hardy Diagnostics, CA) and Amphotericin B (2 μg/ ml) (Mediatech, VA) and grown at 37 °C under microaerophilic conditions (5% O2, 10% CO2, 85% N2) [35 (link), 36 (link)]. For LPS extraction, H. pylori were subcultured into a liquid medium consisting of brain heart infusion broth (BHI, Becton Dickson, MD) supplemented with 5% fetal bovine serum (FBS) and cultured for twenty-four hours on a reciprocal shaker at 37 °C under microaerophilic conditions. Bacteria used for LPS extraction were in logarithmic phase of growth.
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H. pylori strains Sydney strain 1 (SS1) [27 (link)] and G27 (a kind gift from Dr. Guillemin, University of Oregon) were used in this study. H. pylori were routinely maintained on solid medium, Columbia agar (Becton Dickson, MD) supplemented with 5% laked blood (Hardy Diagnostics, CA) and Amphotericin B (2 μg/ ml) (Mediatech, VA) and grown at 37 °C under microaerophilic conditions (5% O2, 10% CO2, 85% N2) [35 (link), 36 (link)]. For LPS extraction, H. pylori were subcultured into a liquid medium consisting of brain heart infusion broth (BHI, Becton Dickson, MD) supplemented with 5% fetal bovine serum (FBS) and cultured for twenty-four hours on a reciprocal shaker at 37 °C under microaerophilic conditions. Bacteria used for LPS extraction were in logarithmic phase of growth.
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