Sgc 7901
The SGC-7901 is a laboratory equipment product manufactured by the Merck Group. It is designed to perform specific functions within a research or testing environment. The core function of the SGC-7901 is to provide precise and reliable measurements or data collection, but a detailed description of its intended use or performance capabilities cannot be provided in an unbiased and factual manner without the risk of extrapolation.
Lab products found in correlation
13 protocols using sgc 7901
Establishment of Cisplatin-Resistant Gastric Cancer Cell Lines
Establishment of Cisplatin-Resistant Gastric Cancer Cell Lines
Establishment of DDP-Resistant Gastric Cancer Cell Lines
DDP-resistant SGC7901/DDP and BGC823/DDP cells were induced from SGC7901 and BGC823 cells, respectively, using a concentration gradient method to increase the half maximal inhibitory concentration (IC50) of DDP. At first, SGC7901 and BGC823 cells were treated with the culture medium containing 0.05 μg/mL DDP for 24 h. Then, the culture medium containing DDP was substituted for the fresh culture medium. When the cell density reached 80%, cells were digested and passage. The cells were treated with 0.05 μg/mL DDP time and again until the cells can be stable passage in such concentration of DDP. Subsequently, the cells were treated with higher concentration of DDP in turn, until the final concentration of DDP reached 1 μg/mL. The cells were cultured in the culture medium containing 1 μg/mL DDP to maintain its drug resistance. Before every experiment, both DDP-resistant gastric cell lines were cultured in drug-free RPMI 1640 medium for 2 weeks.
Culturing Human Gastric Cancer Cell Lines
Establishing Gastric Cancer Drug Resistance
For the drug resistance experiments, we referred a predecessor's research to conduct a habitual stepwise method to generate cisplatin (DDP) and 5-fluorouracil (5-Fu) resistant SGC-7901 cell line.41 DDP and 5-Fu reagents (Sigma-Aldrich, St. Louis, MO, USA) were prepared for the incubation of cultured cells those treated and non-treated with siRNA or pcDNA vector.
Establishment of Cisplatin-Resistant Gastric Cancer Cell Lines
Cultivation and Hypoxic Exposure of Gastric Cell Lines
Establishing 5-FU Resistant Gastric Cancer Cell Line
Cell Culture Conditions for Gastric and Kidney Cell Lines
Gastric and Kidney Cell Cultures
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