Log In Sign up
The largest database of trusted experimental protocols

Hs hprt1 1 sg quantitect primer assay

Manufactured by Qiagen
Visit Supplier

The Hs_HPRT1_1_SG QuantiTect Primer Assay is a pre-designed primer and probe set for the detection and quantification of the human HPRT1 gene transcript. The assay is designed for use with the QuantiTect Probe RT-PCR Kit or other compatible real-time PCR systems.

Automatically generated - may contain errors

Lab products found in correlation

Quantitect primer assay, by Qiagen (2 mentions) Light cycler 480 2 detection system, by Roche (2 mentions) Lightcycler 480 sybr green 1 mix, by Roche (2 mentions) Quantitect reverse transcription kit, by Qiagen (2 mentions) Rneasy plus mini kit, by Qiagen (2 mentions)

Spelling variants of this product

QuantiTect Primer Assays (768 citations) QuantiTect primers (247 citations) QuantiTect (227 citations) HPRT1 (10 citations) Quantitect assays (5 citations)

2 protocols using hs hprt1 1 sg quantitect primer assay

1

Quantifying TFEB and EGR1 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated using the RNeasy Plus mini kit (Qiagen) according to the manufacturer’s instructions. cDNA was synthesized using the QuantiTect Reverse Transcription kit (Qiagen). qPCR was performed with the LightCycler 480 SYBR Green I mix (Roche) using the LightCycler 480 II detection system (Roche). QuantiTect Primer Assays from Qiagen were used to quantify genes of interest. HPRT1 (Hs_HPRT1_1_SG QuantiTect Primer Assay, Qiagen, QT00059066) was used as an endogenous housekeeping control, and results were displayed as fold change compared to control samples. The following probes were used to quantify TFEB and EGR1: Hs_TFEB_1_SG QuantiTect Primer Assay (Qiagen, QT00069951) and Hs_EGR1_1_SG QuantiTect Primer Assay (Qiagen, QT002185051).
Cesana M., Tufano G., Panariello F., Zampelli N., Ambrosio S., De Cegli R., Mutarelli M., Vaccaro L., Ziller M.J., Cacchiarelli D., Medina D.L, & Ballabio A. (2023). EGR1 drives cell proliferation by directly stimulating TFEB transcription in response to starvation. PLOS Biology, 21(3), e3002034.
+ Open protocol
+ Expand
2

Quantitative Analysis of TFEB Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated using the RNeasy Plus mini kit (Qiagen) according to the manufacturer's instructions. cDNA was synthesized using the QuantiTect Reverse Transcription kit (Qiagen). qPCR was performed with the LightCycler 480 SYBR Green I mix (Roche) using the LightCycler 480 II detection system (Roche). QuantiTect Primer Assays from Qiagen were used to quantify genes of interest. HPRT1 (Hs_HPRT1_1_SG QuantiTect Primer Assay, Qiagen, QT00059066) and RPL22 (Hs_RPL22_1_SG QuantiTect Primer Assay, Qiagen, QT00079982) were used as an endogenous housekeeping control, and results were displayed as fold change compared to control samples. The following probes were used to quantify: TFEB (Hs_TFEB_1_SG QuantiTect Primer Assay, Qiagen, QT00069951)
Cesana M., Tufano G., Panariello F., Zampelli N., Soldati C., Mutarelli M., Montefusco S., Grieco G., Sepe L.V., Rossi B., Nusco E., Rossignoli G., Panebianco G., Merciai F., Salviati E., Sommella E.M., Campiglia P., Martello G., Cacchiarelli D., Medina D.L, & Ballabio A. (2024). TFEB controls syncytiotrophoblast formation and hormone production in placenta. Cell death and differentiation.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!