P hydroxybenzoic acid

Manufactured by Yuanye Bio-Technology

Sourced in China

P-hydroxybenzoic acid is a chemical compound that serves as a laboratory reagent. It is a crystalline solid with the chemical formula C6H5OH and a molecular weight of 122.12 g/mol. This substance can be used in various analytical and experimental procedures within a research laboratory setting.

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Lab products found in correlation

11 protocols using p hydroxybenzoic acid

Phytochemical Analysis of Foxtail Millet Husk

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Foxtail millet husk (dry) was acquired from Jinsu Agricultural Technology Corporation (Henan Province, China). The sample was smashed (RS-FS1401 mill, Royalstar Co., Ltd., Hefei, China) sieved (40-mesh), and then stored at −20 °C.
Betaine and L-lactic acid were obtained from Shanghai Macklin Biochemical Co., Ltd., (Shanghai, China). Acetylcholinesterase, α-glucosidase (from Saccharomyces cerevisiae), and standards (vanillic acid, p-hydroxybenzoic acid, p-hydroxybenzaldehyde, caffeic acid, p-coumaric acid, ferulic acid, syringic acid, rutin, and kaempferol, the purity above 98%) were sourced from Shanghai Yuanye Biotechnology Co., Ltd., (Shanghai, China), while ABTS(2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate)), DPPH(2,2′-diphenyl-1-picrylhydrazyl), Folin–Ciocâlteu reagent, Trolox (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid), and TPTZ(2,4,6-tris(2-pyridyl)-s-triazine) were acquired from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Chromatographic methanol and formic acid were purchased from Thermo Fisher Scientific Reagent Co., Ltd., (Waltham, MA, USA). All other reagents, including glycerol, sodium carbonate (Na₂CO₃), sodium acetate, sodium hydroxide (NaOH), sodium nitrite (NaNO₂), glycol, aluminum trichloride (AlCl₃), ferric chloride (FeCl₃), and methanol, were purchased from Tianjin Deen Chemical Reagent Co., Ltd. (Tianjing, China).

Wang C., Li Z., Xiang J., Johnson J.B., Zheng B., Luo L, & Beta T. (2023). From Foxtail Millet Husk (Waste) to Bioactive Phenolic Extracts Using Deep Eutectic Solvent Extraction and Evaluation of Antioxidant, Acetylcholinesterase, and α-Glucosidase Inhibitory Activities. Foods, 12(6), 1144.

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Chromatographic Identification of Phenolic Compounds

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The standards of the chemicals used were of chromatographic grade, (≥98%). For identification purposes, tryptophan, rutin, isofraxidin, kaempferol-3-o-rutinoside, coniferaldehyde, isochlorogenic acid, hesperidin, 3,4-dihydroxybenzoic acid, P-hydroxybenzoic acid, vanillic acid, caffeic acid, vanillin, P-coumaric acid, and ferulic acid were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China).

Gao H., Guo M., Wang L., Sun C, & Huang L. (2022). Identification and Antioxidant Capacity of Free and Bound Phenolics in Six Varieties of Mulberry Seeds Using UPLC-ESI-QTOF-MS/MS. Antioxidants, 11(9), 1764.

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Polyphenol Extraction and Antioxidant Evaluation

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Folin-Ciocalteu reagent, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2′-diphenyl-1-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), were acquired from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). HPLC and MS grade methanol, and formic acid were purchased from Thermo Fisher Scientific Reagent Co., Ltd. (Waltham, Massachusetts, USA). The polyphenol standards, including caffeic acid, chlorogenic acid, protocatechualdehyde, p-hydroxybenzaldehyde, p-hydroxybenzoic acid, p-coumaric acid, protocatechuic acid, ferulic acid, vanillic acid and rutin, were acquired from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). All the other regular chemicals, including sodium carbonate (Na₂CO₃), ferric trichloride (FeCl₃), sodium hydroxide (NaOH), aluminum trichloride (AlCl₃), sodium nitrite (NaNO₂) ethyl acetate and N-hexane, were acquired from Tianjin Deen Chemical Reagent Co., Ltd. (Tianjin, China).

Xiang J., Yuan Y., Du L., Zhang Y., Li C, & Beta T. (2023). Modification on phenolic profiles and enhancement of antioxidant activity of proso millets during germination. Food Chemistry: X, 18, 100628.

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Preparation of Polyphenol Standard Solutions

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Chlorogenic acid (CAS: 327-97-9), neoChlorogenic acid (CAS: 906-33-2), trans-cinnamic acid (CAS: 140-10-3), ferulic acid (CAS: 1135-24-6), p-coumaric acid (CAS: 501-98-4), caffeic acid (CAS: 331-39-5), p-hydroxybenzoic acid (CAS: 99-96-7), epicatechin (CAS: 490-46-0), (+)-catechin (CAS: 154-23-4), (−)-epigallocatechin (CAS: 970-74–1), (−)-gallocatechin (CAS: 3371-27-5), phloretin (CAS: 60-82-2) and phlorizin (CAS: 60-81-1) were purchased from Shanghai Yuanye Bio-technology Co., Ltd (Shanghai, China). All phenolic chemicals were analytical standards, with HPLC purity ≥ 98%.
Stock solutions of polyphenols were obtained by dissolving each polyphenol in methanol containing 5% dimethyl sulfoxide (DMSO). Subsequently, each polyphenol was diluted with sterilized NB medium to the appropriate concentration. All solutions were prepared fresh and filter-sterilized (pore size, 0.22 μm) prior to use.

Zhang Y., Wei J., Qiu Y., Niu C., Song Z., Yuan Y, & Yue T. (2019). Structure-Dependent Inhibition of Stenotrophomonas maltophilia by Polyphenol and Its Impact on Cell Membrane. Frontiers in Microbiology, 10, 2646.

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Quantification of Puerarin and Piperine

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Puerarin and piperine with the purity >99% were purchased from Xian Kaicheng Biological Technology Co., Ltd. (Xian, China). p-Hydroxybenzoic acid (used as internal standard (IS)) was obtained from Shanghai Yuanye Biological Technology Co., Ltd. (Shanghai, China). White pepper obtained from Hainan Province was powdered to pass through an 80-mesh sieve. HPLC-grade methanol was purchased from Merck KGaA (Darmstadt, Germany) and acetic acid from Beijing Chemical Co. (Beijing, China). Ultrapure water was prepared using a Milli-Q gradient water purification system (Millipore, Bedford, MA, USA). All chemicals used in this study were at least of analytical grade.

Liang Y.Z., Chen H.M., Su Z.Q., Hou S.Z., Chen X.Y., Zheng Y.F., Li Y.C., Lin J., Zhan J.Y., Su Z.R, & Fu L.D. (2014). White Pepper and Piperine Have Different Effects on Pharmacokinetics of Puerarin in Rats. Evidence-based Complementary and Alternative Medicine : eCAM, 2014, 796890.

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Polyphenol Standards and Antioxidant Assays

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Acetonitrile (HPLC-grade) was purchased from Adamas reagent, Ltd. (Shanghai, China). All the polyphenol standards (gallic acid, proanthocyanidin B2, epicatechin, proanthocyanidin A2, syringic acid, p-hydroxybenzoic acid, poncirin, ferulic acid, rutin, phlorizin, and methyl hesperidin), purity ≥99%, were purchased from Shanghai yuanye biotechnology Co. Ltd. (Shanghai, China). Vitamin C; 1, 1-diphenyl-2-picrylhydrazyl (DPPH); 2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS); 2, 3, 5-triphenyltetrazolium chloride (TPTZ); triton; tris-HCl; paraformaldehyde; and urethane were purchased from the Beijing Solaibao Technology Co. Ltd. (Beijing, China). Trizol reagent was purchased from TaKaRa (Beijing, China). Hematoxylin and eosin (H&E) kit, Oil Red O (ORO) kit, and a protein extraction kit were obtained from Beyotime Biotechnology (Beijing, China). Antibodies were purchased from Abcam (Shanghai, China).

Tan S., Ke Z., Zhou C., Luo Y., Ding X., Luo G., Li W, & Shi S. (2023). Polyphenol Profile, Antioxidant Activity, and Hypolipidemic Effect of Longan Byproducts. Molecules, 28(5), 2083.

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Pomegranate Peel Bioactive Compounds

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Pomegranate peel was purchased from Hebei Kang’an Biotech. Co. (Anguo, China). p-Nitrophenyl-α-D-glucopyranoside (pNPG) and α-glucosidase (100 U/mg) were obtained from Sigma-Aldrich (Shanghai, China). Acarbose, ellagic acid (EA), and the other compounds including nicotinic acid, isoguanosine, sclareol glycol, triptolide, sclareolide, p-hydroxybenzoic acid, kaempferol, gallic acid, quercetin, and resveratrol were purchased from Yuanye Biotech. Co. (Shanghai, China). All other chemicals used in this study were analytical grade.

Shi R., Zhou N., Zhang H., Gong M, & Han L. (2022). Bioaffinity ultrafiltration coupled with HPLC-ESI-MS/MS for screening potential α-glucosidase inhibitors from pomegranate peel. Frontiers in Nutrition, 9, 1014862.

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Phenolic Acid Standards Acquisition

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Phenolic acid standards (gallic acid, p‐hydroxybenzoic acid, protocatechuic acid, vanillic acid, syringic acid, chlorogenic acid, caffeic acid, p‐coumaric acid, ferulic acid, and erucic acid) were purchased from Shanghai Yuanye Biotechnology (Shanghai, China). Other analytical pure reagents were obtained from Sinopharm Chemical Reagent (Shanghai, China).

Hu J., Vinothkanna A., Wu M., Ekumah J., Akpabli‐Tsigbe N.D, & Ma Y. (2021). Tracking the dynamic changes of a flavor, phenolic profile, and antioxidant properties of Lactiplantibacillus plantarum‐ and Saccharomyces cerevisiae‐fermented mulberry wine. Food Science & Nutrition, 9(11), 6294-6306.

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Phenolic Compound Extraction and Characterization

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Gallic acid, p-hydroxybenzoic acid, ferulic acid, trans-cinnamic acid, p-coumaric acid, benzoic acid, ellagic acid, protocatechuic acid, sinapic acid, caffeic acid, quercetin, rutin, taxifolin, isoquercitrin, catechin, gallocatechin, epicatechin, vitexin, genistin, daidzin, phloroglucinol, quinic acid, and 3,4-dihdyroxyphenylaceticacid were obtained from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Procyanidin B1, procyanidin B2, and kaempferol-3-O-rutinoside were obtained from Chengdu Pusi Biotechnology Co., Ltd. (Chengdu, China). 2,20-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,4,6tri(pyridin-2-yl)-1,3,5-triazine (TPTZ), (±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), fluorescein, 3′,6′-dihydroxyspiro [isobenzofuran-1 (3 H),9′-(9 H) xanthene]-3-one (FL), 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH), and EDTA-2Na were purchased from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Thermo Fisher Scientific (Waltham, MA, USA) provided the methanol (HPLC quality), formic acid (99.9%, LC-MS grade), and acetonitrile (LC-MS grade). Cellulase (T richoderma Vride G, 400 u/mg), hemicellulase (Aspergillus niger, 20 u/mg), and pectinase (Aspergillus niger, 500 u/mg) were obtained from Yuanye Bio-Technology Co., Ltd. (Shanghai, China).

Jiang Q., Wang S., Yang Y., Luo J., Yang R, & Li W. (2023). Profiles of Free and Bound Phenolics and Their Antioxidant Capacity in Rice Bean (Vigna umbellata). Foods, 12(14), 2718.

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Antioxidant Activity Evaluation of Jizi439 BWB

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The Jizi439 BWB was received from Yueqing Agricultural Science and Technology Co., Ltd. (Handan, China). Tris-HCl buffer was obtained from Solarbio Science and Technology Co. (Beijing, China). DPPH (2,2-diphenyl-1-picrylhydrazyl) and 2,2′-Azinobis (3-ethylbenzothiazoline-6-sulfonate) (ABTS) free radical were acquired from Sigma-Aldrich (St. Louis, MO, USA). Folin-Ciocalteu reagent and NaCO₃ were purchased from Hushi chemical Co. (Shanghai, China). Standards, including Gallic acid, p-hydroxybenzoic acid, vanillic acid, p-coumaric acid, ferulic acid, syringic acid, caffeic acid, protocatechuic acid, chlorogenic acid, catechin, luteolin, and apigenin, were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China).

Chen X., Sun K., Zhuang K, & Ding W. (2022). Comparison and Optimization of Different Extraction Methods of Bound Phenolics from Jizi439 Black Wheat Bran. Foods, 11(10), 1478.

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