Total RNA was extracted by Vazyme Kit (RC101-01) (Vazyme, Nanjing, China). RNA was reverse transcribed into cDNA by HiScript III RT SuperMix 100 for qPCR (+gDNAwiper) (Vazyme, Nanjing, China). ChamQSYBR qPCR Master Mix (Vazyme Q711-02; Vazyme, Nanjing, China) was used for polymerase chain reaction in StepOne Real-time PCR System (Applied Biosystems, Waltham, MA, USA). The relative level was calculated with 2−ΔΔCt.
Hiscript 3 rt supermix 100 for qpcr gdnawiper
Manufactured by Vazyme
Sourced in China
HiScript III RT SuperMix 100 for qPCR (+gDNAwiper) is a reverse transcription kit for quantitative PCR (qPCR) applications. It includes a reverse transcriptase enzyme and a gDNAwiper component to remove genomic DNA contamination.
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2 protocols using hiscript 3 rt supermix 100 for qpcr gdnawiper
1
RNA Extraction and qPCR Analysis
2
Spinal Cord Injury RNA Extraction
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We collected total RNA from the proximal 5 mm spinal cord tissues after SCI with TRIzol reagent (Gibco, Grand Island, NY, USA). HiScript III RT SuperMix 100 for qPCR (+gDNA wiper) (Vazyme, Nanjing, China) was used to reverse transcript RNA into complementary DNA. Real-time polymerase chain reaction was performed with ChamQ SYBR qPCR Master Mix (Vazyme) on a StepOne Real-time PCR System (Applied Biosystems, Foster City, CA, USA). Primer sequences were listed in Additional Table 1
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