Acrylamide (AAm), N-hydroxysuccinamide, 4’Aminofluorescein isomer I, 5(6)-Carboxyfluorescein, Fluorescein disodium salt, FITC, 3-(trimethoxysilyl) propyl methacrylate and Ethylene glycol were purchased from Acros Organics. Pyruvic acid and 3-aminopropyl methacrylamide hydrochloride (3AP) were purchased from Sigma-Aldrich. Tetramethylethylenediamine (TEMED) was purchased from ThermoScientific. Ammonium persulfate (APS) was purchased from Aqua Solutions. Polyethylene(glycol) diacrylate (PEGDA; Mw = 400) was purchased from PolySciences Inc. 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) was purchased from Advanced Chemtec. Hydrochloric acid (37.5%) and glycine were purchased from Fisher Scientific. Glass microscope slides (3” × 1” × 1.2 mm) were purchased from Fisherbrand. Ethanol (EtOH) was purchased from Decon Laboratories Inc. Water (dd-H2O) was deionized using Milli-Q Advantage A-10 water purification system (Millipore, U.S.A.). When required, degassing was performed by sparging with a continuous argon stream for 15 min for small volumes and 2 h for large volumes. Coverglass slips (Thickness #1, Diameter 25mm) were purchased from Electron Microscopy Sciences. Rain-X hydrophobic spray manufactured by ITW (Global Bands, TX) was purchased from Home Depot.
5 6 carboxyfluorescein
Manufactured by Thermo Fisher Scientific
Sourced in Belgium
5(6)-Carboxyfluorescein is a fluorescent dye used in various laboratory applications. It is a derivative of fluorescein and emits green fluorescence when excited with blue or ultraviolet light. The dye is often used as a labeling agent for biomolecules, such as proteins and nucleic acids, to facilitate detection and visualization in various analytical techniques.
Automatically generated - may contain errors
Lab products found in correlation
Hydrochloric acid (hcl), by Thermo Fisher Scientific (1 mentions)
Ethylene glycol, by Thermo Fisher Scientific (1 mentions)
Milli q advantage a10 water purification system, by Merck Group (1 mentions)
Glycine, by Thermo Fisher Scientific (1 mentions)
Fluorescein disodium salt, by Thermo Fisher Scientific (1 mentions)
Fluorescein isothiocyanate (fitc), by Thermo Fisher Scientific (1 mentions)
3 trimethoxysilyl propyl methacrylate, by Thermo Fisher Scientific (1 mentions)
Tetramethylethylenediamine temed, by Thermo Fisher Scientific (1 mentions)
Polyethylene glycol diacrylate pegda mw 400, by Polysciences (1 mentions)
Glass microscope slide, by Thermo Fisher Scientific (1 mentions)
Pyruvic acid, by Merck Group (1 mentions)
2 protocols using 5 6 carboxyfluorescein
1
Fluorescent Hydrogel Synthesis Protocol
2
Peptide Synthesis and Purification
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Peptides DEIVNKVL (Vps2 residues 165–172) and DEIVNKVLDEIGVDLNSQLQ (Vps2 residues 165–184) were prepared by solid-phase synthesis on a Prelude X peptide synthesizer (Protein Technologies, Inc., Tucson, AZ) using standard procedures and Fmoc chemistry (Chan and White, 2000 ). Unlabeled peptides were N-terminally acetylated and C-termini were produced as carboxyamides. For peptides used in fluorescence polarization assays, 5 (6)-carboxyfluorescein (Acros Organics, Geel, Belgium) was coupled to the N-terminal α-amine by standard coupling conditions. Following cleavage from resin by TFA, the peptides were precipitated with ice-cold ether, washed thoroughly with ether, and dried overnight under vacuum. Peptides were then HPLC purified on a Phenomenex 5 µm-C4 Jupiter column (10 × 250 mm, 300 Å) at 5 ml/min over a 15 min gradient (20–80% ACN,0.1% TFA). Peptide quality was verified by LC-MS using a FortisBIO 5 µm C4 column (4.6 × 150 mm, 300 Å) coupled to an Agilent 6100 Series Single Quadrupole mass spectrometer.
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