HEK-293T cells were co-transfected with miR-3150b mimic or mimic NC and the Luciferase miRNA Expression Reporter (Promega, Madison, Wisconsin, USA) comprising 3ʹ untranslated region (UTR) of GOLPH3 (wild or mutant type). At 48 hours post-transfection, the relative luciferase activity was calculated.
Luciferase mirna expression reporter
Manufactured by Promega
Sourced in United States
The Luciferase miRNA Expression Reporter is a laboratory tool used to detect and quantify microRNA (miRNA) expression levels. It operates by incorporating a luciferase reporter gene that responds to the presence of specific miRNA molecules, allowing researchers to measure miRNA activity in cells or tissues.
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Lab products found in correlation
2 protocols using luciferase mirna expression reporter
1
Regulate GOLPH3 Expression by miR-3150b
2
MiR-212-3p Regulation of SOX11 3'UTR
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JeKo-1 and Z-138 cells (4×103 cells/well) were seeded into a 96-well plate, The SOX11 mutated and SOX11 wild-type (WT) 3′UTR was also added to the Luciferase miRNA expression reporter (Promega Inc.) and miR-212-3p mimics, SOX11 mutated and SOX11 wild-type (WT) were transfected into the cells using Lipofectamine 3000, as aforementioned. After 48 h of transfection Firefly and Renilla luciferase activities in the cells were determined using a dual luciferase reporter assay kit (Promega Corporation). The Firefly luciferase activities were normalized to Renilla luciferase activity and the relative luciferase activity of Firefly (to Renilla) were reported in the present study. Each assay was repeated thrice.
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