Log In Sign up
The largest database of trusted experimental protocols

Recombinant il 23

Manufactured by BioLegend
Visit Supplier

Recombinant IL-23 is a cytokine produced using recombinant DNA technology. It is a heterodimeric protein composed of two subunits, the IL-23 p19 and IL-12 p40 subunits. IL-23 plays a role in the regulation of immune responses and inflammation.

Automatically generated - may contain errors

Lab products found in correlation

Recombinant mouse il 6, by Thermo Fisher Scientific (1 mentions) Recombinant il 2, by Thermo Fisher Scientific (1 mentions) Foxp3 transcription factor kit, by Thermo Fisher Scientific (1 mentions) Ifn γ, by Thermo Fisher Scientific (1 mentions) Cd4 cd25 regulatory t cell isolation kit, by Miltenyi Biotec (1 mentions) Gm csf, by BioLegend (1 mentions) Everolimus, by Selleck Chemicals (1 mentions) Interleukin 6 (il 6), by BioLegend (1 mentions) Ifn γ, by BioLegend (1 mentions) Rat igg1, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

IL-23 (65 citations) Recombinant mouse IL-23 (11 citations) Recombinant murine IL-23 (3 citations) Human recombinant IL-23 (2 citations)

4 protocols using recombinant il 23

1

Isolation and Expansion of Murine Tregs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Treg cells derived from Foxp3Cre-YFP mice were isolated using a CD4+CD25+ Regulatory T Cell Isolation kit (Miltenyi) and were cultured in the presence of 2,000 U ml–1 recombinant IL-2 (Peprotech) and anti-CD3/CD28 beads of the mouse Treg cell expansion kit (Miltenyi) with a ratio of two beads per cell. In addition, recombinant mouse IL-6 (50 ng ml–1; Peprotech), IFNγ (100 ng ml–1; Peprotech) and recombinant IL-23 (20 ng ml–1; BioLegend) were added, and the cells were cultured for 5 d. For short-term stimulation, Treg cells were, after the 5-d expansion, stimulated with recombinant IL-23 (50 ng ml–1; BioLegend) for 30 min. Cells were stained with LIVE/DEAD Zombie NIR for 15 min at 4 °C and fixed using 1:1 fixation concentrate and diluent of the Foxp3 transcription factor kit (Thermo Fisher). The cells were then stained intracellularly with antibodies in perm buffer for 30 min at room temperature in the dark.
Wertheimer T., Zwicky P., Rindlisbacher L., Sparano C., Vermeer M., de Melo B.M., Haftmann C., Rückert T., Sethi A., Schärli S., Huber A., Ingelfinger F., Xu C., Kim D., Häne P., Fonseca da Silva A., Muschaweckh A., Nunez N., Krishnarajah S., Köhler N., Zeiser R., Oukka M., Korn T., Tugues S, & Becher B. (2024). IL-23 stabilizes an effector Treg cell program in the tumor microenvironment. Nature Immunology, 25(3), 512-524.
+ Open protocol
+ Expand
2

Novel G6PD Inhibitors in Cancer Therapy

Check if the same lab product or an alternative is used in the 5 most similar protocols
Unless noted otherwise, all reagents, small molecule inhibitors and chemotherapies were of the highest grade and purchased from Sigma-Aldrich. Lonidamine was purchased from Tocris biosciences. Everolimus was purchased from SelleckChem. Doxorubicin, PI103 were purchased from LC Labs. All chemotherapeutics and small molecule inhibitors were dissolved in DMSO to a stock concentration of 10 mM and kept frozen except for compounds used for animal studies, which were freshly prepared at the indicated concentrations. Recombinant IL-23, IFN-γ, GM-CSF, IL-6 and IL-8 were purchased from Biolegend. The G6PD inhibitor CB-83 was originally reported in Preuss et al. by the Chembridge ID#6138697, which was purchased from ChemBridge (50 (link)). The G6PD inhibitors, compounds 59, 60, and 61, were obtained from ChemDiv; purity (of >95%) and identity was confirmed by the medicinal chemistry core at Sanford Burnham Prebys by LC-MS and 1H NMR. Analog 59 was 2-(pyridin-3-yl)-3-(p-tolyl)quinazolin-4(3H)-one. Analog 60 was 3-(3-methoxyphenyl)-2-(pyridin-3-yl)quinazolin-4(3H)-one, Analog 61 was 3-(2-methoxyphenyl)-2-(pyridin-3-yl)quinazolin-4(3H)-one.
Goldman A., Khiste S., Freinkman E., Dhawan A., Majumder B., Mondal J., Pinkerton A.B., Eton E., Medhi R., Chandrasekar V., Rahman M.M., Ichimura T., Gopinath K.S., Majumder P., Kohandel M, & Sengupta S. (2019). Targeting Tumor Phenotypic Plasticity and Metabolic Remodeling in Adaptive Cross-Drug Tolerance. Science signaling, 12(595), eaas8779.
+ Open protocol
+ Expand
3

Intradermal IL-23 and CCL2 Injection

Check if the same lab product or an alternative is used in the 5 most similar protocols
One µg of recombinant IL‐23 (BioLegend) or CCL2 (BioLegend) was injected daily into a defined, marked area of the back skin. PBS was injected as a control.
Novoszel P., Holcmann M., Stulnig G., De Sa Fernandes C., Zyulina V., Borek I., Linder M., Bogusch A., Drobits B., Bauer T., Tam‐Amersdorfer C., Brunner P.M., Stary G., Bakiri L., Wagner E.F., Strobl H, & Sibilia M. (2021). Psoriatic skin inflammation is promoted by c‐Jun/AP‐1‐dependent CCL2 and IL‐23 expression in dendritic cells. EMBO Molecular Medicine, 13(4), e12409.
+ Open protocol
+ Expand
4

IL-23 Stimulation of Mouse Skin Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse dorsal skin cells were stimulated with 40 ng/ml recombinant IL‐23 (Biolegend) for 20 h, with 10 μg/ml anti CD200R1 (OX131, Absolute Antibody) or isotype control (rat IgG1, eBioscience).
Linley H., Jaigirdar S., Mohamed K., Griffiths C.E, & Saunders A. (2022). Reduced cutaneous CD200:CD200R1 signaling in psoriasis enhances neutrophil recruitment to skin. Immunity, Inflammation and Disease, 10(7), e648.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!