Trap5b elisa kit

Manufactured by Immunodiagnostic Systems

The TRAP5b ELISA Kit is a laboratory diagnostic tool used to measure the levels of TRAP5b (Tartrate-Resistant Acid Phosphatase 5b) in biological samples. TRAP5b is an enzyme that is released during the process of bone resorption and can be used as a biomarker for bone metabolism. The kit employs the enzyme-linked immunosorbent assay (ELISA) technique to quantify the TRAP5b concentration in the sample.

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2 protocols using trap5b elisa kit

Serum TRAP5b Detection Protocol

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Mouse blood was collected through intracardiac puncture immediately after euthanasia. The blood was transferred to a 1.5 mL tube and left at room temperature for 30 min. The clot was removed by centrifuging at 7,000 rpm in a refrigerated centrifuge for 15 min. The resulting supernatant was aliquoted and stored in a −80 °C freezer. For the detection of serum TRAP5b, a TRAP5b ELISA Kit (Immunodiagnostic Systems, SB-TR103) was used according to the manufacturer’s instructions.

Zhao Y., Teng H., Deng Y., Sheldon M., Martinez C., Zhang J., Tian A., Sun Y., Nakagawa S., Yao F., Wang H, & Ma L. (2023). Long noncoding RNA Malat1 inhibits Tead3-Nfatc1–mediated osteoclastogenesis to suppress osteoporosis and bone metastasis. Research Square.

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Serum TRAP5b Quantification in Mice

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Mouse blood was collected through intracardiac puncture immediately after euthanasia. The blood was transferred to a 1.5 mL tube and left at room temperature for 30 min. The clot was removed by centrifuging at 7000 rpm in a refrigerated centrifuge for 15 min. The resulting supernatant was aliquoted and stored in a −80 °C freezer. For the detection of serum TRAP5b, a TRAP5b ELISA Kit (Immunodiagnostic Systems, SB-TR103) was used according to the manufacturer’s instructions.

Zhao Y., Ning J., Teng H., Deng Y., Sheldon M., Shi L., Martinez C., Zhang J., Tian A., Sun Y., Nakagawa S., Yao F., Wang H, & Ma L. (2024). Long noncoding RNA Malat1 protects against osteoporosis and bone metastasis. Nature Communications, 15, 2384.

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