Pre-coated Matrigel chambers (BD Biosciences) were used for the invasion assays according to the manufacturer's instructions. After transfection of pCMV3-CDH16 plasmid and empty pCMV3 plasmid at 37°C for 48 h, the cells were collected. A total of 1×104 BCPAP cells were seeded into the upper chamber of the Transwell apparatus in serum-free RPMI-1640, while RPMI-1640 supplemented with 10% FBS was added to the lower chamber. After incubation at 37°C for 24 h, cells adhering to the lower surface were fixed with 100% methanol for 20 min at room temperature and stained with 5% Giemsa solution for 20 min at room temperature. The number of infiltrated cells was calculated under a light microscope (Olympus Corporation IX71) on five randomly selected fields (×100 magnification).
Pre coated matrigel chambers
Manufactured by BD
Pre-coated Matrigel Chambers are laboratory equipment designed for cell migration and invasion assays. They consist of cell culture inserts pre-coated with a layer of Matrigel, a gelatinous protein mixture that mimics the extracellular matrix. These chambers provide a standardized platform for studying the ability of cells to migrate through or invade the Matrigel barrier.
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Lab products found in correlation
3 protocols using pre coated matrigel chambers
1
Matrigel Invasion Assay for BCPAP Cells
2
LKB1-Mediated Cancer Cell Migration
3
LKB1-Mediated Cancer Cell Migration
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HeLa cells with inducible LKB1 were placed in the upper chamber of the Transwell Membrane Inserts or pre-coated Matrigel Chambers from BD Biosciences (San Jose, CA). To induce LKB1, 100 μM zinc sulfate was added to the low chamber. After 24 h, migratory or invasive cells were stained with 1% crystal violet and quantified under a microscope. Assays with TC-1/shR-Ctrl and TC-1/shR-LKB1 cells were conducted similarly except the lower chambers contained regular culture medium.
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