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Dulbecco s modified eagle media dmem

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Dulbecco's modified eagle media (DMEM) is a cell culture medium commonly used for the in vitro maintenance and growth of various cell types. It provides a balanced salt solution and essential nutrients to support cell survival and proliferation. DMEM is a widely-used and well-established media formulation in the field of cell biology and biotechnology.

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Lab products found in correlation

Pen strep, by Lonza (1 mentions) Raw264, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Penicillin streptomycin, by Lonza (1 mentions)

Spelling variants of this product

DMEM media (34 citations) Dulbecco’s Modified (7 citations) Dulbeccos’ modified eagle’s media DMEM (2 citations)

2 protocols using dulbecco s modified eagle media dmem

1

Culturing Human Alveolar and Liver Cells

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Mammalian cell lines [human alveolar basal epithelial cells (A549) (ATCC CCL-185) or human liver epithelial cells with hepatocellular carcinoma (HepG2) (ATCC Hb-8065)] were grown at 37 °C and 5% CO2 in Dulbecco’s modified eagle media (DMEM) (Lonza, Basel, Switzerland) with 10% (v/v) heat-inactivated sterile foetal bovine serum (Scientifix Life, North Ryde, Australia), (100 U penicillin + 100 µg/mL) Penstrep (Lonza, Basel, Switzerland).
Galletta M., Reekie T.A., Nagalingam G., Bottomley A.L., Harry E.J., Kassiou M, & Triccas J.A. (2020). Rapid Antibacterial Activity of Cannabichromenic Acid against Methicillin-Resistant Staphylococcus aureus. Antibiotics, 9(8), 523.
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2

Overexpression of Oncogenes and Slit2 in Mammary Tumor Cells

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MVT1 cells (VEGF and c-MYC oncogenes overexpressing mouse mammary tumor
cell line) (14 (link)) and MDA-MB-231 cells
overexpressing Slit2 (231-Slit2) or vector control (231-Vec) were a generous
gift from Dr. Ostrowski (The Ohio State University) and Dr. Hinck (University of
California, Santa Cruze) respectively. RAW264.7 and MDA-MB-231 cells were
purchased from ATCC, USA. All the cells cultured in Dulbecco’s modified
eagle media (DMEM) (Lonza, USA) supplemented with 10% fetal bovine serum
(Sigma-Aldrich, USA) and 1% Penicillin/ Streptomycin (Lonza, USA).
Ahirwar D.K., Charan M., Mishra S., Verma A.K., Shilo K., Ramaswamy B, & Ganju R.K. (2021). Slit2 inhibits breast cancer metastasis by activating M1-like phagocytic and anti-fibrotic macrophages. Cancer research, 81(20), 5255-5267.
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