BALF cytokine concentrations were measured using ProcartaPlex Luminex platform (ThermoFischer, USA). The measurements were performed in duplicates (25 μl each) with a custom multiplex panel detecting the following mouse cytokines: IFNγ, TNFα, RANTES, IL-6, IL-10, IL-1α, IL-1β, IP-10, MIP1α, and MCP-1. Measurements were performed using Luminex MAGPIX instrument, and results were analyzed with Xponent 4.2 software according to manufacturer instructions.
Procartaplex luminex platform
Manufactured by Thermo Fisher Scientific
Sourced in United States
The ProcartaPlex Luminex platform is a multiplex assay system that allows for the simultaneous detection and quantification of multiple analytes from a single sample. The platform utilizes color-coded magnetic microspheres coated with capture antibodies specific to the target analytes. This enables the analysis of a wide range of biomolecules, including proteins, cytokines, and other analytes, in a high-throughput manner.
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Lab products found in correlation
2 protocols using procartaplex luminex platform
1
Multiplexed Cytokine Profiling in BALF
2
Multiplex Cytokine Profiling in BALF
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The BALF cytokine concentrations were measured using the ProcartaPlex Luminex platform (Thermo Fischer Scientific, MA, USA). The measurements were performed two times (25 μl of each sample) with a custom multiplex panel detecting the following mouse cytokines: IFNγ, TNFα, RANTES, IL-6, IL-10, IL-1α, IL-1β, IP-10, MIP1α, and MCP-1/CCL2. The measurements were performed using the Luminex MAGPIX instrument, and results were analyzed with Xponent 4.2 software according to the instructions from the manufacturer.
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