Cytosine d arabinofuranoside ara c

Manufactured by Merck Group

Sourced in United States

Cytosine-D-arabinofuranoside (Ara-C) is a synthetic nucleoside analogue used as a laboratory reagent. It functions as an inhibitor of DNA synthesis.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Ara-C (363 citations) Cytosine (40 citations) Cytosine β-D-arabinofuranoside (Ara-C), (33 citations) Cytosine-D-arabinofuranoside (14 citations) Cytosine arabinofuranoside (10 citations) Cytosine 1-β-d-arabinofuranoside (Ara-C; (8 citations) Cytosine arabinofuranoside (Ara-C) (4 citations)

5 protocols using cytosine d arabinofuranoside ara c

Culturing Hippocampal Neurons from Transgenic Mice

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cultured hippocampal neurons were prepared as described (Yang et al., 2009 (link)) with some modifications. Briefly, we dissected hippocampus from App^-/--Gad67^+/GFP and WT-Gad67^+/GFP littermates of P0/1 pups, trypsinized the tissue at 37°C for 30 min, and then gently triturated in culture medium containing 10% heat-inactivated fetal bovine serum using fire-polished pipettes, before centrifuging at 1000 rpm for 10 min. Cells were resuspended in Neurobasal Medium supplemented with B27 and L-glutamine (Invitrogen, Carlsbad, CA), and plated at 1–2 × 10⁴ cells/ml onto 13 mm poly-L-lysine coated coverslips. One day after plating, 4 M cytosine-D-arabinofuranoside (Ara-C) (Sigma) was added to prevent astrocyte proliferation. Cultures of 12–14 DIV were used for patch clamp recordings.

Chen M., Wang J., Jiang J., Zheng X., Justice N.J., Wang K., Ran X., Li Y., Huo Q., Zhang J., Li H., Lu N., Wang Y., Zheng H., Long C, & Yang L. (2017). APP modulates KCC2 expression and function in hippocampal GABAergic inhibition. eLife, 6, e20142.

+ Open protocol

+ Expand

Dissociation and Maintenance of Rat Hippocampal and Cortical Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary cultures of dissociated hippocampal or cortical cells were prepared and maintained as previously described [45 (link)]. Briefly, hippocampi and neocortices from pooled postnatal day 0 (P0) or P1 male and female rat pups were dissociated and plated onto tissue culture plastic or on glass coverslips precoated with poly-L-lysine (Sigma), at different densities depending on the endpoint to be analyzed (detailed information provided in the respective method section). Cells were maintained in Neurobasal A media supplemented with B27 and 2 mM Glutamax (all from Life Technologies), except for cultures used in the proteomics experiments, for which cells were maintained in Neural Q supplemented with GS21 (both from Sigma). Cytosine-D-arabinofuranoside (araC; 5 μM, Sigma) was added to the medium on day in vitro (DIV) 4 to prevent overgrowth of astrocytes. Half the medium was replaced with fresh media (without araC) twice weekly.

Stamou M., Grodzki A.C., van Oostrum M., Wollscheid B, & Lein P.J. (2018). Fc gamma receptors are expressed in the developing rat brain and activate downstream signaling molecules upon cross-linking with immune complex. Journal of Neuroinflammation, 15, 7.

+ Open protocol

+ Expand

Visualization of Cardiomyocyte DNA Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols

BrdU (30 μM, Sigma) was added to the medium of cardiomyocytes to examine DNA synthesis. To inhibit DNA polymerization, cytosine-D-arabinofuranoside (araC) (10 μM, Sigma) was added to the dishes. Mitosis was determined by visualization of nuclei stained positive for an antibody to phosphorylated histone H3 (H3P). An antibody against aurora B kinase (Sigma-Aldrich, Cat.# A5102) was used to visualize cytokinesis. Signals were visualized with secondary antibodies conjugated to donkey anti-rabbit (FITC, Jackson Immunoresearch). BrdU was stained with a 5-Bromo-2′-deoxy-uridine Labeling and Detection Kit I (Roche Applied Science) and nuclei were visualized with 4′,6′-diamidino-2-phenylindole (DAPI, Invitrogen). Fluorescence imaging was performed with an Olympus BX41 (Olympus America Inc., Melville, NY, U.S.A.) equipped with epiflouresence microscopy, and images were recorded using a digital camera with MagnaFire^™ 2.1 software.

Wu S.Z., Li Y.L., Huang W., Cai W.F., Liang J., Paul C., Jiang L., Wu Z.C., Xu M., Zhu P, & Wang Y. (2017). Paracrine effect of CXCR4-overexpressing mesenchymal stem cells on ischemic heart injury. Cell biochemistry and function, 35(2), 113-123.

+ Open protocol

+ Expand

Culturing Embryonic Rat Superior Cervical Ganglia

Check if the same lab product or an alternative is used in the 5 most similar protocols

Superior cervical ganglia (SCGs) were isolated from embryonic day 17 Sprague-Dawley rat embryos (Hilltop Labs). Primary neurons were cultured in tri-chamber dishes as previously described [48 ,49 (link)]. Multi-well or 35-mm plastic tissue culture dishes (Falcon) or optical plastic dishes (Ibidi) were coated with 500 μg/ml of poly-DL-ornithine (Sigma Aldrich) and 10 μg/ml of natural mouse laminin (Invitrogen). After coating, two sets of grooves were etched on the dishes. A silicone grease-coated tri-chamber (Tyler Research) was placed on top of a drop of 1% methylcellulose (in neuronal medium) covering the groves. Ganglia were trypsinized and triturated, and approximately 2/3 of an SCG was plated in the S compartment. Neurons were maintained in complete neuronal medium: Neurobasal medium (Gibco) supplemented with 100 ng/ml nerve growth factor 2.5S (Invitrogen), 2% B27 (Gibco) and 1% penicillin and streptomycin with 2 mM glutamine (Invitrogen). 2 to 3 days after seeding, 1 mM cytosine-D-arabinofuranoside (AraC; Sigma-Aldrich) was added for at least 2 days to eliminate non-neuronal cells. Neurons were cultured for 14–21 days prior to experiments.

Koyuncu O.O., MacGibeny M.A., Hogue I.B, & Enquist L.W. (2017). Compartmented neuronal cultures reveal two distinct mechanisms for alpha herpesvirus escape from genome silencing. PLoS Pathogens, 13(10), e1006608.

+ Open protocol

+ Expand

Culturing Rat Embryonic SCG Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols

SCG neurons from rat embryos (embryonic day 17 [E17]) were cultured in trichambers as previously described.⁶⁹ Briefly, SCGs were dissociated with trypsin (2.5 mg/mL, Sigma-Aldrich, The Woodlands, TX, USA) and plated on poly-O-ornithine and laminin-coated dishes with media containing neurobasal media supplemented with 2% B-27, 100 ng/mL nerve growth factor (NGF), and 1% penicillin-streptomycin-glutamine (Thermo Fisher Scientific, Rockford, IL, USA). Approximately two-thirds of a single ganglion was placed for the S (soma) compartment of the trichamber. Three days after seeding, culture medium was treated with 0.1 mM cytosine-d-arabinofuranoside (Ara C) (Sigma-Aldrich, The Woodlands, TX, USA) for at least 2 days to eliminate dividing, nonneuronal cells. Culture media were replaced every 5 days, and neurons were incubated at 37°C with 5% CO₂.

Maturana C.J., Verpeut J.L., Pisano T.J., Dhanerawala Z.M., Esteves A., Enquist L.W, & Engel E.A. (2020). Small Alphaherpesvirus Latency-Associated Promoters Drive Efficient and Long-Term Transgene Expression in the CNS. Molecular Therapy. Methods & Clinical Development, 17, 843-857.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!