5 mm broadband

¹H and ¹³C NMR spectra of glucan were recorded at 27°C on a Bruker 5 mm broadband, with a spectrometer (Bruker Avance 600 MHz (German)), which operated at 126 MHz for ¹³C NMR and 600 MHz for ¹H NMR. The sample (20 mg) was dissolved in D₂O (99.8%, 0.5 mL), lyophilized and re-dissolved again in D₂O (0.5 mL). The sample was finally centrifuged to remove excessive un-dissolved sample before analysis. All the experiments were recorded using standard Bruker software. FT-IR spectra were recorded on a Nicolet 6700 Thermo Scientific FT-IR spectrometer (USA) with detector (DTGS) in a range of 4000–400 cm⁻¹. The sample measured on KBr discs as a film.

High resolution ¹H spectra were recorded on a Bruker AVIII nuclear magnetic resonance (NMR) spectrometer equipped with a 5 mm broad-band fluorine observation (BBFO) probe head operating at 400 MHz ¹H Larmor frequency. All experiments were carried out at room temperature. The instrument's standard pulse sequence program (zg30) was used with the following parameters: 2-s acquisition time, 20-ppm spectral width, and 5-s relaxation delay. Deuterated chloroform and 5 mm NMR tubes were purchased from Eurisotop (Saint-Aubin, France). In total, 3,072 repetition experiments were performed leading to a total acquisition time of 6 h. After data processing and phase and baseline corrections, the areas of the peaks of interest were determined by integration, and the molar ratio of lipids was calculated with relative integrations.