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Platelia eia

Manufactured by Bio-Rad
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Sourced in Germany

The Platelia EIA is a laboratory equipment product manufactured by Bio-Rad. It is an enzyme immunoassay (EIA) system used for the detection and quantification of specific analytes in biological samples.

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Platelia (17 citations) Platelia Aspergillus EIA (12 citations) Platelia Aspergillus EIA kit (3 citations) Platelia Aspergillus GM EIA (3 citations) Platelia Aspergillus enzyme immunoassay (EIA) (3 citations)

2 protocols using platelia eia

1

Aspergillus Galactomannan Detection in BALF

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BALF Aspergillus GM was determined by the Platelia EIA (Bio-Rad Laboratories, Munich, Germany) in clinical routine at the Medical Clinic of the Research Center Borstel, Germany according to the manufacturer’s instructions. BALF samples were processed in accordance to the manufactures protocol. While our analysis primarily focused on the recommended 1.0 ODI cut-off for BALF, we also evaluated a 0.5 GM ODI cut-off, following previous evidence that the 0.5 ODI cut off is preferable in patients on mold-active antifungals (Eigl et al., 2015 (link), 2017 (link)).
Salzer H.J., Prattes J., Flick H., Reimann M., Heyckendorf J., Kalsdorf B., Obersteiner S., Gaede K.I., Herzmann C., Johnson G.L., Lange C, & Hoenigl M. (2018). Evaluation of Galactomannan Testing, the Aspergillus-Specific Lateral-Flow Device Test and Levels of Cytokines in Bronchoalveolar Lavage Fluid for Diagnosis of Chronic Pulmonary Aspergillosis. Frontiers in Microbiology, 9, 2223.
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2

Quantifying Fungal Biomarkers in BALF

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GM concentrations of BALF were determined routinely by the Platelia EIA (Bio-Rad Laboratories) in our clinical laboratory. The optical density (OD) index cutoff of 1.0 was applied to the evaluation of diagnostic performance for the GM tests (34 (link)). The BDG tests with BALF were carried out according to the manufacturer’s instructions. The average rate of OD change for all points between 0 and 40 min was interpolated with the results of standard curve to determine the amount of BDG (35 (link)). Negative and positive controls were included in each run of the assays.
Zhang L., Lu F., Wang Y., Ji J., Xu Y., Huang Y., Zhang M., Li M., Xia J, & Wang B. (2023). Methodological comparison of bronchoalveolar lavage fluid-based detection of respiratory pathogens in diagnosis of bacterium/fungus-associated pneumonia in critically ill patients. Frontiers in Public Health, 11, 1168812.
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