Synchron cx5

Manufactured by Beckman Coulter

Sourced in United States

The Synchron CX5 is a clinical chemistry analyzer designed for routine and specialized tests in medical laboratories. It is capable of performing a wide range of biochemical assays to aid in the diagnosis and monitoring of various health conditions.

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Mek 7222k, by Nihon Kohden (1 mentions)

10 protocols using synchron cx5

Comprehensive Blood Analysis Protocol

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All studied subjects were subjected to complete history taking (including personal history, history of blood transfusion (1st and frequency), history of drug intake and history of splenectomy and its age). Thorough clinical examination stressing on presence of pallor, jaundice and scars of splenectomy.
Blood sample and laboratory investigations: five milliliters (ml) of venous blood were withdrawn from the cubital vein and divided as follows: two ml of blood were put in a tube containing EDTA for complete blood count (CBC) measured with Pentra – 80 automated blood counter (ABX– France – Rue du Caducee-Paris Euromedecine-BP-7290.34184 Montpellier-Cedex 4.), estimation of HbF% by Hb electrophoresis (automated analyzer (minilite) MNL320350-Italy) and for detection of GNAI2, DUSP5 and ARRB1 mRNA expression by RT-PCR. The remaining 3 ml blood was transferred into plain tube, separated by centrifugation and stored at -20°C for determination of ALT, AST, urea, creatinine on auto-analyzer (SYNCHRON CX5) from Beckman (Beckman, instrument Inc., Scientific Instrument Division, Fullerton, CA92634 - 3100) and ferritin by ELISA kits, Ramco Laboratories Inc, Stafford, Texas, USA.

Elghobashy Y.A., Assar M.F., Mahmoud A.A., Monem A Eltorgoman A, & Elmasry S. (2020). The relation between mitogen activated protein kinase (MAPK) pathway and different genes expression in patients with beta Thalassemia. Biochemistry and Biophysics Reports, 24, 100836.

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Plasma Biomarker Analysis in Rats

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An automated chemistry analyzer (Synchron CX5, Beckman Coulter, Brea, CA) was used to assess the triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), pyruvate, and C-peptide levels in the plasma of rats. The serum insulin level was detected by ELISA.

Zhang B., Yuan Y., Xin J., Chen M., Wang Z., Li X, & Xue T. (2022). Study of Water- and Organic-Soluble Extracts from Trichosanthes on Type 1 Diabetes Mellitus. Journal of Diabetes Research, 2022, 3250016.

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Automated Hematology and Serum Biochemistry

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Blood collected in EDTA coated vials was analyzed using MS-9 automatic hematology analyzer (Melet Schloesing Ltd., France), shortly after its collection. Blood samples were collected for serum chemistry analysis in tubes lacking anticoagulant and placed at room temperature for at least 90 min prior to centrifugation; after centrifugation at 1600 g for 10 min, serum was collected and biochemical parameters were measured using fully automated random access clinical chemistry analyzer (Beckman Synchron CX5, USA).

Yadav N.K., Saini K.S., Hossain Z., Omer A., Sharma C., Gayen J.R., Singh P., Arya K.R, & Singh R.K. (2015). Saraca indica Bark Extract Shows In Vitro Antioxidant, Antibreast Cancer Activity and Does Not Exhibit Toxicological Effects. Oxidative Medicine and Cellular Longevity, 2015, 205360.

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Blood Analysis: Hematology and Serum Chemistry

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Blood collected in EDTA coated vials was analyzed using MS-9 automatic hematology analyzer (Melet Schloesing Ltd., France), shortly after its collection. Blood samples were collected for serum chemistry analysis in tubes lacking anticoagulant and placed at room temperature for at least 90 min prior to centrifugation; after centrifugation at 1600g for 10 min., serum was collected and biochemical parameters were measured using fully automated random access clinical chemistry analyzer (Beckman Synchron CX5, USA).

Yadav N.K., Arya R.K., Dev K., Sharma C., Hossain Z., Meena S., Arya K.R., Gayen J.R., Datta D, & Singh R.K. (2017). Alcoholic Extract of Eclipta alba Shows In Vitro Antioxidant and Anticancer Activity without Exhibiting Toxicological Effects. Oxidative Medicine and Cellular Longevity, 2017, 9094641.

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Hepato-renal Biomarker Analysis in Mice

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Blood samples were collected for serum chemistry analysis in tubes lacking anticoagulant and placed at RT for at least 90 min prior to centrifugation at 1600 g for 10 min. Levels of different hepato-renal factors in the blood of mice were measured using fully automated random access clinical chemistry analyser (Beckman Synchron CX5, USA).

Arya R.K., Singh A., Yadav N.K., Cheruvu S.H., Hossain Z., Meena S., Maheshwari S., Singh A.K., Shahab U., Sharma C., Singh K., Narender T., Mitra K., Arya K.R., Singh R.K., Gayen J.R, & Datta D. (2015). Anti-breast tumor activity of Eclipta extract in-vitro and in-vivo: novel evidence of endoplasmic reticulum specific localization of Hsp60 during apoptosis. Scientific Reports, 5, 18457.

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Metabolic Profiling of Gerbils

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When the animals were 1–1.2 years old and had offsprings, before being euthanized, the animals were tested for the FPG after 16h fasting; then the gerbils were anesthetized, whole blood samples were collected into anticoagulant tubes and non-anticoagulant tube from the orbital sinus. The serum in non-anticoagulant tube were separated and frozen at -80°C for ELISA tests. Plasma separated from anticoagulant tubes and serum were using for biochemical analsyis by Synchron cx5 (Beckman, USA) and MEK-7222K (NIHON KOHDEN, Japan). After blood collection, the gerbils were killed by giving an overdose of pentobarbital. Skeletal muscle, adipose tissue, liver, kidney and pancreas were collected. Each collected tissue was divided into three portions, one of which was fixed in formalin for histological analysis; two were stored at -80°C for Quantitative Real-time PCR (qPCR) and Western blot analysis.

Li X., Lu J., Wang Y., Huo X., Li Z., Zhang S., Li C., Guo M., Du X, & Chen Z. (2016). Establishment and Characterization of a Newly Established Diabetic Gerbil Line. PLoS ONE, 11(7), e0159420.

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Dietary Potassium Modulation: Physiological Effects

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Control (1.2% K⁺), low-K⁺ (0% K⁺), and high-K⁺ (5% K⁺) diets were obtained from TestDiet (St. Louis, MO) and were prepared by modifying the AIN-93M semipurified diet, as previously described (4 (link)). The 0% K⁺ diet was used as the base, and tribasic K⁺-citrate was added to generate the 1.2% K⁺ and 5% K⁺ diets. After a 2-day period of adapting to the 1.2% K⁺ powder diet, during which time mice were also allowed to adapt to the metabolic cages, the diet was changed to 0% or 5% K⁺ for some animals, whereas the control group continued to receive the 1.2% diet. On days 1 and 4 after switching to these diets, mice were placed in metabolic cages for urine collection. At the end of day 4, mice were euthanized for urine and blood collection. The concentrations of urinary and plasma electrolytes were determined using a Synchron CX5 (Beckman Coulter, Miami, FL). The plasma aldosterone concentration was measured by ELISA (DRG), and plasma renin activity was measured by RIA (REN-CT2, RADIM). For only a particular experiment, K⁺ was added to the diet as KCl, to obtain a high-K⁺ diet (5%) with high Cl⁻ content instead of high citrate content.

Castañeda-Bueno M., Cervantes-Perez L.G., Rojas-Vega L., Arroyo-Garza I., Vázquez N., Moreno E, & Gamba G. (2014). Modulation of NCC activity by low and high K+ intake: insights into the signaling pathways involved. American Journal of Physiology - Renal Physiology, 306(12), F1507-F1519.

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Blood Biomarker Analysis in Metabolic Intervention

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Blood samples were collected in the fasting state on one day at baseline, 6 months and 24 months of the intervention, and stored at −80 °C. The measurements were performed at the clinical laboratory at the Pennington Biomedical Research Center. Fasting glucose and insulin concentrations were measured using an immunoassay with chemiluminescent detection on the Immulite analyzer (Diagnostic Products Corporation, Los Angeles CA). HbA1c was measured on a Synchron CX5 (Beckman Coulter, Brea CA). We calculated HOMA-IR using fasting glucose and insulin levels.^{28 (link)}

Heianza Y., Sun D., Li X., DiDonato J.A., Bray G.A., Sacks F.M, & Qi L. (2018). Gut Microbiota Metabolites, Amino Acid Metabolites, and Improvements in Insulin Sensitivity and Glucose Metabolism: the POUNDS Lost Trial. Gut, 68(2), 263-270.

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Analysis of Liver and Kidney Functions

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Blood samples were collected from the control and treated mice for analysis of liver and kidney functions at the end of the experiment following mice sacrificing. The blood urea nitrogen, serum creatinine, alanine transaminase and aspartate transaminase levels were determined by using a fully automated random access clinical chemistry analyzer (Beckman Synchron CX5, Foster, CA, USA).

Hong M., Li J., Li S, & M.Almutairi M. (2019). Acetylshikonin Sensitizes Hepatocellular Carcinoma Cells to Apoptosis through ROS-Mediated Caspase Activation. Cells, 8(11), 1466.

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Lipid Profile Estimation Protocol

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Total serum cholesterol, triglycerides, HDL-cholesterol and LDL-cholesterol were estimated by using Synchron cx5 autoanalyzer (Beckman, USA). The atherogenic index was calculated using the formula of Schulpis and Karikas [20 (link)] as follows: Atherogenic Index of Plasma (AIP)=Log (TG/HDLC), Triglycerides and HDL-cholesterol in AIP reflect the balance between the atherogenic and protective lipoproteins.

Haggag B.S., Hasanin A.H., Raafat M.H, & Abdel Kawy H.S. (2014). Lamotrigine Decreased Hippocampal Damage and Improved Vascular Risk Markers in a Rat Model of Pentylenetetrazole Induced Kindling Seizure. The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology, 18(3), 269-278.

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