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Osteocalcin

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Osteocalcin is a protein found in bone and dentin. It is a key regulator of bone mineralization and calcium homeostasis.

Automatically generated - may contain errors

Lab products found in correlation

Lsm 780, by Zeiss (2 mentions) Bs 1134r, by Bioss Antibodies (1 mentions) Bs 1310r, by Bioss Antibodies (1 mentions) Ab28364, by Abcam (1 mentions) Endomucin v 7c7, by Santa Cruz Biotechnology (1 mentions) Bs 1110r, by Bioss Antibodies (1 mentions) Bs 0470r, by Bioss Antibodies (1 mentions) Runx2, by Bioss Antibodies (1 mentions) Cd105, by Bioss Antibodies (1 mentions) Bs 0579r, by Bioss Antibodies (1 mentions) Bs 23258r, by Bioss Antibodies (1 mentions) Cxcr4 d1s7w, by Cell Signaling Technology (1 mentions) Phospho vegfr2 bs 2674r, by Bioss Antibodies (1 mentions) Osteopontin, by Bioss Antibodies (1 mentions) Emmprin, by Abcam (1 mentions) Mmp 9, by Abcam (1 mentions)

2 protocols using osteocalcin

1

Immunofluorescence Analysis of Bone Markers

Cited 1 time
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Immunofluorescence was performed as previously described [28 (link),29 (link)]. Briefly, the bone sections were incubated with individual primary antibodies against mouse CD31 (ab28364; Abcam), endomucin (V.7C7; Santa Cruz), Ki67 (AF7617; R&D), beta-catenin (8480, CST), osterix (bs-1110R; Bioss), osteocalcin (bs-0470R; Bioss), Runx2 (bs-1134R; Bioss), DLL4 (bs-6044R; Bioss), Notch1 (bs-1335R; Bioss), Noggin (bs-2975R; Bioss), CXCR4 D1S7W; Cell Signaling Technology), integrin αvβ3 (bs-1310R; Bioss), CD90 (bs-20640R; Bioss), CD105 (bs-0579R; Bioss), CD271 (bs-0161R; Bioss), OPN (bs-23258R; Bioss), and phospho-VEGFR2(bs-2674R; Bioss) overnight at 4 °C. Subsequently, the samples were incubated with fluorescence-coupled secondary antibodies for 1 h at 37 °C in the dark. Images were collected using a confocal laser microscope (LSM 780; Carl Zeiss).
Tang Y., Luo K., Chen Y., Chen Y., Zhou R., Chen C., Tan J., Deng M., Dai Q., Yu X., Liu J., Zhang C., Wu W., Xu J., Dong S, & Luo F. (2021). Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering. Bioactive Materials, 6(7), 2039-2057.
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2

Immunohistochemical Analysis of Alveolar Bone Healing

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Streptavidin-Peroxidase kit (Zhongshan, Beijing, China) was used according to the instructions. Polyclonal antibody EMMPRIN and MMP-9 (dilution 1:150, Abcam, UK) were applied. Osteocalcin (OCN) and osteopontin (OPN) (dilution 1:100, Bioss, China) were performed to determine the expression of these proteins in the alveolar bone healing. PBS was obtained as negative control.
Li J., Sheng Z., Sun J., Wang R, & Yu X. (2022). Characterizations of alveolar repair after mandibular second molar extraction: an experimental study in rats. Journal of Applied Oral Science, 30, e20220010.
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