Ovcar3

We were purchased the SKOV3 human epithelial ovarian cancer cell line from the Korean Cell Line Bank (KCLB, Seoul, Korea), and the OVCA429 and OVCA433 cell line were provided by the Korea Gynecologic Cancer Bank through the Bio and Medical Technology Development Program of the Ministry of Science, Information and Communication Technology, and Future Planning (MSIP, Seoul, Korea). The HEK293, TOV112D and OVCAR3 cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA), and the A2780 cell line was procured from the European Collection of Cell Cultures (ECACC, London, UK). SKOV3, TOV112D and A2780 cells were cultured in RPMI-1640 medium (Welgene, Seoul, Korea), and HEK293, OVCAR3, OVCA429 and OVCA433 cells were cultured in Dulbecco’s Modified Eagle’s Medium (Welgene, Seoul, Korea). All culture media were supplemented with 10% foetal bovine serum and 1% penicillin/streptomycin, and cell lines were maintained at 37 °C in a humidified atmosphere of 5% CO₂ and 95% air. Culture medium was replaced with fresh medium every 2–3 days, and cells were used between Passages 5 and 10. OVCA429 and OVCA433 cells were used between passages 10 and 20. The subtypes of the epithelial ovarian cancer cell lines are as follows: SKOV3, serous; TOV112D, endometrioid; A2780, non-specified; OVCAR3, carcinoma; OVCA429, serous; and OVCA433, serous [29 (link)].

A2780 and OVCAR-3 human ovarian cancer cell lines were obtained from the American Type Culture Collection (Manassas, VA, USA). A2780 and OVCAR-3 cells were cultured in DMEM (Welgene, Daegu, South Korea) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 mg/mL streptomycin (all from Welgene) and incubated at 37 °C under a humidified 95%/5% (v/v) mixture of air and CO₂. To validate the phenotypic characteristics of the ovarian cancer cell lines A2780 and OVCAR-3, we performed Western blotting analyses and identified EpCAM expression (Supplementary Figure S3).

Human ovarian cancer cell lines (SK-OV-3, CA-OV-3, OVCAR3, and TOV-21G) were purchased from the American Type Culture Collection (Manassas, VA, USA). SK-OV-3, CA-OV-3, OVCAR3, and TOV-21G cells were grown in Roswell Park Memorial Institute (RPMI) 1640 supplemented with 10% FBS, 100 U/ml penicillin, and 100 μg/ml streptomycin (WelGENE, Daegu, South Korea) in a humidified atmosphere of 5% CO₂ at 37 °C.