Arginase 1

Western blots were done on BMDM lysates using Cell Signaling Technology Cell Lysis Buffer 10x (#9803) supplemented with 0.5% SDS and protease inhibitor tablet (Roche, #04693124001). ~5μg protein was loaded and PVDF membranes were probed with antibodies specific to iNOS (Novus Biologicals, #NB300-605), Arginase-1 (Proteintech, #16001-1-AP), β-tubulin (Proteintech, #10094-1-AP), β-actin (Thermo Fisher Scientific, # PA5-85291), DGKα (Proteintech, #11547-1-AP), and Phospho-(Ser) PKC Substrate (Cell Signaling, #2261). Quantification of immunoblots was performed by first normalizing band intensities to β-tubulin or β-actin to control for loading variability. Relative intensities were then calculated by normalizing to PBS control conditions.

Antibodies: Antibodies against fibronectin and collagen I were purchased from Cell Signaling Technology (MA, USA). Antibodies against Sart1, Arginase 1, β-actin, Gapdh and F4/80 were purchased from Proteintech (Hubei, China). Recombinant IL-4 was purchased from BioLegend (CA, USA). Antibodies against STAT6, p-STAT6 and PPAR-γ were obtained from Cell Signaling Technology (MA, USA).
Lipids: Cholesterol and DSPC were acquired from Sigma-Aldrich, Inc. (St. Louis, MO). mPEG2000-DMG (MW2660) was purchased from NOF Co., Ltd. (Kawasaki Japan). The cationic lipidoid C12-200 was generated through ring opening of epoxides by amine substrates with a previously reported method 21 (link). Clodronate liposomes (anionic) were purchased from FormuMax (CA, USA).
Inhibitors: Inhibitors targeting STAT6 (AS1517499) and PPAR-γ (T0070907) were purchased from MedChemExpress (NJ, USA).
BLM was obtained from Hisun Pharmaceutical Co., Ltd (Zhejiang, China). An RT-PCR assay kit was supplied by Takara (Liaoning, China). A hydroxyproline assay kit was obtained from BioVision (CA, USA). Cell Counting Kit-8 was purchased from Dojindo Chemical technology Co. Ltd. (Shanghai, China).