Ultra low plates

Manufactured by Corning

Sourced in United States

Ultra Low plates are a specialized laboratory product designed for a variety of scientific applications. They feature a low-binding surface that minimizes protein and biomolecule adsorption, making them suitable for various assays and experiments that require precise and consistent results. The plates are constructed with high-quality materials to ensure durability and reliable performance.

Automatically generated - may contain errors

Lab products found in correlation

Cyan adp, by Beckman Coulter (1 mentions) Mammocult medium, by STEMCELL (1 mentions) Epidermal growth factor (egf), by R&D Systems (1 mentions) Insulin, by R&D Systems (1 mentions) Fibroblast growth factor (fgf), by R&D Systems (1 mentions) Heparin, by Merck Group (1 mentions) Dmem f12 medium, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Ultra-low attachment surface tissue culture plates Costar Ultra low attachment U-bottom 96-well plates 96-well ultra-low adherence plates Costar ultra-low-attachment cell culture plates Ultra-low attachment 6 multi-well plates Ultra-low adhesion (polyHEMA coated) 96 well plates 96-well ultra-low attachment culture plates Six-well ultra-low cluster plates Ultra-low attachment 24-well culture plates Round‐bottomed ultra‐low attachment 96‐well plates

3 protocols using ultra low plates

Sphere Cell Characterization

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After sorting, the SP cells and MP cells were cultured for 5 weeks in the previously mentioned medium in 6-well Ultra Low plates (Corning). The spheres were divided into single cells, stained with fluorescent-labelled ABA and VVA (Vector Laboratories) for 15 min at room temperature, and detected using CyAn ADP (Beckman Coulter).

Zhao R., Liu X., Wang Y., Jie X., Qin R., Qin W., Zhang M., Tai H., Yang C., Li L., Peng P., Shao M., Zhang X., Wu H., Ruan Y., Xu C., Ren S, & Gu J. (2016). Integrated glycomic analysis of ovarian cancer side population cells. Clinical Proteomics, 13, 32.

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Tumorsphere Formation Assay

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Tumor cells were suspended in Mammocult medium (Stem Cell, Canada) at appropriate concentration and added into ultra-low plates (Corning, USA). The tumorspheres (sphere diameter ≧70 μm) were counted after 5 to 7 days of culture.

Wang S., Ma L., Wang Z., He H., Chen H., Duan Z., Li Y., Si Q., Chuang T.H., Chen C, & Luo Y. (2021). Lactate Dehydrogenase-A (LDH-A) Preserves Cancer Stemness and Recruitment of Tumor-Associated Macrophages to Promote Breast Cancer Progression. Frontiers in Oncology, 11, 654452.

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Cultivation of SP and MP Cells

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SP cells or MP cells were seeded at 2000 per well in 24-well Ultra Low plates (Corning, New York, NY, USA) in DMEM/F12 medium (Gibco) containing 20 ng/ml fibroblast growth factor, 20 ng/ml epidermal growth factor, 2% B27, insulin (R&D Systems, Minneapolis, MN, USA) and 2 mg/ml heparin (Sigma-Aldrich) and cultured for 21 days.

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