Pseudomonas spp. isolation and identification were performed using the modified conventional TS EN ISO 13720 standard [18 ]. First, 250 µL of the swab sample containing each liquid medium was taken and placed in 2 mL of Pseudomonas Broth (Z699101 Merck, Darmstadt, Germany) and incubated at 22 ± 2 °C for 44 ± 4 h (Pre-enrichment). Subsequently, 0.1 mL of the suspension in Pseudomonas Broth was taken and spread onto Pseudomonas Agar (CM 559 Oxoid, Basingstoke, UK) containing Pseudomonas CFC Selective Supplement (SR103 Oxoid). The plates were incubated at 22 ± 2 °C for 44 ± 4 h. After incubation, suspected Pseudomonas spp. were transferred to Tryptic Soy Agar (CM 131, Oxoid, Basingstoke, UK) for purification. Biochemical tests such as Gram staining, oxidase test, catalase test, and fluorescence properties with UV light (365 nm) were applied to confirm Pseudomonas strains [9 ,18 ].
Pseudomonas broth
Manufactured by Merck Group
Sourced in Germany
Pseudomonas Broth is a culture medium used for the growth and maintenance of Pseudomonas bacteria. It provides the necessary nutrients and conditions for the proliferation of Pseudomonas species in a laboratory setting.
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Lab products found in correlation
2 protocols using pseudomonas broth
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Pseudomonas Isolation and Identification Protocol
2
Bacterial Growth and Enumeration
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A strain of B. cereus (26 B, own collection; isolated from raw cow milk) and a reference strain of S. aureus (Czech Collection of Microorganisms, Masaryk University, Brno; CCM 6188) were multiplied in a broth (10 g Pepton, 10 g Lab Lemco Poder, 5 g NaCl, 1000 ml distilled water) at 36 °C for 24 h. Two strains of P. fluorescens, ZB 66 (own collection; isolated from raw cow milk) and CCM 2826 (reference strain) were multiplied in a Pseudomonas broth containing 1% of glycerol and 0.1% of milk powder (Merck) at 30 °C for 24 h. Then the counts of bacteria (CFU/ml) were determined (ČSN EN ISO 7218, 2008) .
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