Nanoscope iiia controller

¹H NMR spectra were measured on a Bruker ARX 400 MHz spectrometer using DMSO-d6 (for TA-Br), CDCl₃ (for TA-PGMA) and D₂O (for TP, TP-E, and TP-ColIV) as the solvents with tetramethylsilane (Me₄Si) as an internal standard. Thermogravimetric analysis measurement of TA-Br-Gd was performed on a Thermal Gravimetric Analyzer (TG 209, NETZSCH). GPC measurement of TA-PGMA was performed on a Waters GPC system with DMSO as the eluent. The concentrations of Gd³⁺ in TP and TP-Gd were investigated using inductively coupled plasma mass spectrometry (ICP-MS, Thermo Scientific iCAP 6000 series). The fluorescence intensities of rhodamine B modified ColIV and TP-ColIV-RhB were characterized using a fluorescence spectrophotometer (Hitachi F-7000). Dynamic light scattering (DLS) measurements of polycation/miRNA complexes were performed with a Zetasizer Nano ZS equipped with a laser of wavelength 633 nm at a 173° scattering angle (Malvern Instruments, Southborough, MA). Atomic force microscopy (AFM) studies were carried out with the Dimension Icon model with a Nanoscope IIIa controller (Bruker, Santa Barbara, CA), where samples were imaged using the ScanAsyst mode. Gel electrophoresis was implemented in a Sub-Cell system (Bio-Rad Laboratories), and then a UV transilluminator and BioDco-It imaging system (UVP Inc.) was employed to record miRNA bands.

AFM was used to determine the size and particle morphology of Ferrlecit and generic SFG. Samples were diluted 1:20 using MilliQ water to reach a final concentration of 0.625 mg Fe per mL. 15 μL of the 1:20 solution was deposited onto a freshly cleaved mica disk (0.25 inch in diameter). After 30-s incubation, the mica surface was rinsed with water and dried by centrifugation. Each specimen was placed on the vacuum chuck of the Digital Instruments (Bruker Nano, Santa Barbara, CA, USA) NanoScope AFM system fitted with NanoScope IIIA Controller with Phase Extender Module and Dimension 3100 Large Sample AFM with type G Scanner. TappingMode™ was used to capture height and phase data types for 1 μm² fields of view at a resolution of 512 × 512 pixels for images to be used for measurement. In three independent runs of specimen preparation and imaging for each lot, a total of 216 images were evaluated to analyze 2460 well-separated particles (950 for Ferrlecit lot #D2C283A, 534 for Ferrlecit lot #D2C593A, and 976 for generic SFG lot #132296.1) whose height could be readily measured.

SAP solutions were made at 1 mg/mL in sterile water, exposed to UV if indicated, and then diluted to 0.1 mg/ml in sterile water and placed in a sonicator bath for 30 min. Next, 50 μl of each solution was deposited onto freshly cleaved mica and allowed to adsorb to the surface for 5 min. Excess liquid was blotted from the mica using filter paper, and samples were stored at 4°C overnight to finish drying. Images were obtained using a Bruker multimode AFM with NanoScope IIIa controller. The AFM probe had a resonance frequency of 325 kHz and a force constant of 40 N/m (MikroMasch #HQ:NSC15/AL BS). Scanning was performed in tapping mode with a 2 Hz scan rate and 512 samples/line. AFM height images were corrected for low‐frequency noise and tilt by flattening them with NanoScope Analysis software v1.5 (Bruker).