Peroxidase glucose oxidase

Postprandial abomasal emptying and plasma glucose and insulin curves were determined on d 6. Acetaminophen was added to the morning MR meal at 0.13 g/ kg of BW 0.75 and its appearance in plasma was used as an indicator of abomasal emptying (Schaer et al., 2005; (link)MacPherson et al., 2016) (link). Plasma was analyzed for concentrations of acetaminophen [Paracetamol (ac-etaminophen) Assay Kit-K8002; Cambridge Life Sciences Ltd., Cambridgeshire, UK] and insulin (Mercodia Bovine Insulin ELISA; Mercodia, Uppsala, Sweden) as described by MacPherson et al. (2016) (link). Plasma glucose was determined using an enzymatic assay with peroxidase glucose oxidase and dianisidine dihydrochloride (Sigma-Aldrich, St. Louis, MO). Inter-and intra-assay coefficients of variation for plasma acetaminophen, insulin, and glucose were 0.9 and 1.3%, 4.7 and 4.6%, and 3.3 and 0.9%, respectively.

Plasma samples were analyzed for hormone and glucose concentrations. Plasma hormone concentrations were measured following the time-resolved fluoroimmunoassay technique, which was previously described by Sugino et al. (2004) . Plasma GLP-1 concentration was measured using a solid-phase competition immunoassay with europium-labeled human GLP-1 and polystyrene microtiter strips (Nalgene Nunc Int., Tokyo, Japan) coated with anti-rabbit γ-globulin (Inabu et al., 2017) . Intra-and interassay coefficients of variation were 4.7 and 5.3%, respectively, and the least detectable level was 0.007 ng/mL. The plasma concentration of insulin was measured using a solid-phase competition immunoassay with europium-labeled bovine insulin and polystyrene microtiter strips coated with anti-guinea pig γ-globulin (Inabu et al., 2017) . Intra-and interassay coefficients of variation were 2.1 and 3.1%, respectively, and the least detectable level was 0.14 ng/mL. Plasma glucose concentration was analyzed using an enzymatic method with peroxidase glucose oxidase (Sigma-Aldrich).