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Lab tek chambered coverglass w cvt

Manufactured by Thermo Fisher Scientific
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The Lab-Tek chambered Coverglass W/CVT is a laboratory equipment designed for cell culture and microscopy applications. It consists of a removable coverglass with a chamber that allows for the controlled growth and observation of cells. The product provides a standardized and convenient platform for conducting various cell-based experiments and analyses.

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Lab products found in correlation

Lsm 510 meta, by Zeiss (2 mentions) Draq5, by Merck Group (1 mentions) Fv1000 confocal microscopy, by Olympus (1 mentions)

Close spelling variants of this product

Chambered coverglass Lab-Tek

3 protocols using lab tek chambered coverglass w cvt

1

Imaging Fungal Strains with sGFP

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Microscopy experiments were performed as described previously (Bayram et al., 2019 (link)). Strains expressing sGFP were grown in Lab-Tek chambered Coverglass W/CVT (Thermo Scientific, 155360) in 400 μl GMM with required supplements for 16 h at 25 °C. DRAQ5 (Sigma) with 1:10,000 dilution was used for nuclear staining 30 min prior to imaging under microscope. Microscopic images were captured using the Olympus FV1000 confocal microscopy in 60x magnification.
Karahoda B., Pfannenstiel B.T., Sarikaya-Bayram Ö., Dong Z., Wong K.H., Fleming A.B., Keller N.P, & Bayram Ö. (2023). The KdmB-EcoA-RpdA-SntB (KERS) chromatin regulatory complex controls development, secondary metabolism and pathogenicity in Aspergillus flavus. Fungal genetics and biology : FG & B, 169, 103836.
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2

Live-cell Fluorescence Imaging of Strains

Cited 1 time
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Strains expressing sGFP and mRFP were grown in Lab-Tek chambered coverglass W/CVT (Thermo Scientific, Cat# 155360) in 400 μl GMM for 16 h at 30ºC. Microscopic images were captured as described earlier (39 (link)).
Karahoda B., Pardeshi L., Ulas M., Dong Z., Shirgaonkar N., Guo S., Wang F., Tan K., Sarikaya-Bayram Ö., Bauer I., Dowling P., Fleming A.B., Pfannenstiel B.T., Luciano-Rosario D., Berger H., Graessle S., Alhussain M.M., Strauss J., Keller N.P., Wong K.H, & Bayram Ö. (2022). The KdmB-EcoA-RpdA-SntB chromatin complex binds regulatory genes and coordinates fungal development with mycotoxin synthesis. Nucleic Acids Research, 50(17), 9797-9813.
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3

Fluorescent Protein Localization in Fungi

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GFP and mRFP-tagged strains were inoculated (5 × 103 spores) in 500 μl liquid GMM media with supplements and cultured in Lab-Tek Chambered Coverglass W/CVT (Thermo Scientific) for 16 hours at 30 °C. Localisations of the proteins were captured using the Zeiss LSM 510 META inverted confocal microscope.
Frawley D., Karahoda B., Sarikaya Bayram Ö, & Bayram Ö. (2018). The HamE scaffold positively regulates MpkB phosphorylation to promote development and secondary metabolism in Aspergillus nidulans. Scientific Reports, 8, 16588.
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