Murine MS1 and SVR were cultured at 37 °C with 5% CO2, in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich Inc., Saint Louis, MO, USA), enriched with 10% fetal bovine serum (Atlanta Biologicals, Lawrenceville, GA, USA), 1% complex of antibiotics-L-glutamine (10,000 IU/mL penicillin, 10,000 μg/mL streptomycin, and 29.2 mg/mL; Mediatech Inc., Manassas, VA, USA), and 1.5% L-glutamine 200 mM (Sigma-Aldrich Inc., Saint Louis, MO, USA), changed every 24 h [11 (link)]. MS1 and SVR were we incubated at either 37 °C or 39 °C for 24 h or 48 h. MS1 cells were murine pancreatic microvascular endothelial cells immortalized with a temperature-sensitive large T antigen, and SVR was derived from MS1 through the introduction of oncogenic H-ras.
L glutamine 200 mm
Manufactured by Merck Group
Sourced in United States, Italy
L-glutamine 200 mM is a laboratory reagent that provides a source of the amino acid L-glutamine. L-glutamine is a common nutrient required for cell culture and other biological applications. The 200 mM concentration provides a standardized solution for precise supplementation.
Automatically generated - may contain errors
Lab products found in correlation
Bovine serum albumin (bsa), by Merck Group (4 mentions)
Leibovitz s l 15 medium, by Thermo Fisher Scientific (3 mentions)
α thioglycerol, by Merck Group (3 mentions)
L ascorbic acid, by Merck Group (3 mentions)
Hoechst 33342, by Merck Group (3 mentions)
Fructose, by Merck Group (3 mentions)
α mem glutamax medium, by Thermo Fisher Scientific (2 mentions)
Insulin transferrin selenium 100, by Thermo Fisher Scientific (2 mentions)
Live dead fixable far red stain, by Thermo Fisher Scientific (2 mentions)
Amphotericin b 250 μg ml, by Merck Group (2 mentions)
Eosin y, by Merck Group (2 mentions)
Dulbecco s modified eagle s medium, by Merck Group (1 mentions)
Penicillin, by Corning (1 mentions)
Streptomycin, by Corning (1 mentions)
Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions)
Paraffin wax, by Carlo Erba (1 mentions)
Penicillin streptomycin 100, by Merck Group (1 mentions)
Penicillin streptomycin 100x, by Merck Group (1 mentions)
Amphotericin b, by Merck Group (1 mentions)
Ldh kit, by Merck Group (1 mentions)
8 protocols using l glutamine 200 mm
1
Murine Endothelial Cell Culture Conditions
2
Culturing Human Leukemia HL60 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human leukaemia cell line HL60 (promyelocitic cells) was supplied by Dr. Angeles Alonso-Moraga (Genetic Department, University of Cordoba, Spain). HL60 cells were grown in a RPMI-1640 suspension medium (Invitrogen, Waltham, MA, USA). This medium was supplemented with antibiotics 100× (Sigma, Darmstadt, Germany), L-glutamine 200 mM (Sigma, Darmstadt, Germany) and 10% heat-inactivated foetal bovine serum (Linus, Cultek, Madrid, Spain). The experiments were run in a 5% CO2 humidified atmosphere at 37 °C using a CO2 Incubator (Shel Lab, Sheldon, OR, USA). The HL60 cells were subcultured every 2–3 days to maintain their logarithmic growth and were allowed to grow for 48 h before use. The cultures were plated at a density of 12.5 × 104 cells mL−1 in 40 mL culture bottles (25 cm2).
3
Cell culture protocol for viability
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Conventional culture dishes that are 50 mm in diameter were purchased from Falcon (Sigma-Aldrich, Milan, Italy). Leibovitz’s L-15 medium, α-MEM GlutaMAX medium, insulin transferrin selenium (ITS) 100×, and LIVE/DEAD Fixable Far Red stain were purchased from Invitrogen (Milan, Italy). Penicillin-streptomycin 100×, amphotericin B 250 μg/mL, bovine serum albumin, L-ascorbic acid, L-glutamine 200 mM, Hoechst 33342, fructose, and α-thioglycerol were purchased from Sigma-Aldrich (Milan, Italy). Mayers’s hematoxylin and paraffin wax were purchased from Carlo Erba (Milan, Italy).
4
Comparative analysis of cell culture systems
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lumox gas-permeable culture dishes and conventional culture dishes 50mm in diameter were from Sarstedt (Nümbrecht, Germany) and Falcon (Sigma-Aldrich, Milan, Italy) respectively. Leibovitz’s L-15 medium, α-MEM Glutamax medium (code number 32571), Insulin transferrin selenium (ITS) 100x, Live/dead Fixable far red stain were purchased from Invitrogen (Milan, Italy). Penicillin streptomycin 100x, Amphotericin B 250μg/ml, Bovine serum albumin (BSA), L-Ascorbic acid, L-Glutamine 200mM, Hoechst 33342, Fructose, α-thioglycerol and Eosin-Y were purchased from Sigma Aldrich (Milan, Italy). Mayers’s hematoxylin and paraffin wax were from Carlo Erba (Milan, Italy).
5
Optimized Tissue Culturing Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lumox culture dishes with gas-permeable bottom 50 mm in diameter (PD) were purchased from Sarstedt AG & Co. KG (Nuembrecht, Germany). Conventional dishes 50 mm in diameter were purchased from Falcon (Sigma-Aldrich, Milan, Italy). Leibovitz's L-15 medium, α-MEM Glutamax medium (code number 32571), insulin transferrin selenium (ITS) 100×, and live/dead fixable Far Red stain were purchased from Invitrogen (Milan, Italy). Penicillin streptomycin (Pen-Strep) 100×, amphotericin B 250 μg/ml, bovine serum albumin BSA, L-ascorbic acid, L-glutamine 200 mM, Hoechst 33342, fructose, α-thioglycerol, and eosin-Y were purchased from Sigma Aldrich (Milan, Italy). Mayers's hematoxylin and paraffin wax were from Carlo Erba (Milan, Italy). Estradiol and progesterone solid phase enzyme immunoassay kits were obtained from DRG (Marburg, Germany). LDH kit was obtained from Merck (Sigma-Aldrich, Milan, Italy). The tissue chopper was purchased from McIlwain, Mickle Laboratory Engineering Company Ltd (Surrey, UK). Pump tubing was purchased from Cole-Parmer (Milan, Italy) and the peristaltic pump from Ismatec (Enco, Venice, Italy).
6
Oxidative Stress Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Histopaque 1077 and 1119, RPMI-1640 culture medium, l -glutamine 200 mM, penicillin–streptomycin 100×solution, fibronectin, fetal calf serum, bovine serum albumin, and phosphate buffered saline (PBS) were all acquired from Sigma-Aldrich (St. Louis, MO, USA). Sodium Azide (NaN3) was obtained from LabChem, Inc. (Pittsburgh, PA, USA). Dichlorofluorescein diacetate (DCF-DA) precursor dye was obtained from Molecular Probes (Eugene, OR, USA). The peroxyl free radical generator 2,2′-Azobis-(2-amidinopropane) dihydrochloride (AAPH) was obtained from Wako Chemicals (Richmond, VA, USA).
7
Thioacetamide-Induced Liver Fibrosis Study
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Thioacetamide was purchased from Sigma–Aldrich, USA. DAB substrate and DAB staining solution were purchased from BD Pharmingen (San Diego, CA 92121, USA). The HepG2 and FL83B cell lines were purchased from ATCC (Manassas, USA). Dulbecco’s modified Eagle’s medium (DMEM) was purchased from Thermo Scientific, USA. L-Glutamine (200 mM) was purchased from Sigma–Aldrich, USA. A hydroxyproline assay kit was purchased from Chondrex (WA, USA). Anti-alpha smooth muscle actin antibody-Lot:GR283004-24 (Abcam, Cambridge, UK), goat anti-rabbit IgG H&L (Alexa Fluor1 488) Lot:GR306624-1 (Abcam, Cambridge, UK), hepatitis B core antigen Lot:#SF2406841H (Invitrogen, Thermo Fisher Scientific, South Korea), and goat anti-rabbit IgG (HRP) Lot:GR247075-7 (Abcam, Cambridge, UK) were used. A plasmid DNA purification kit was purchased from Intron Biotechnology (Lynnwood WA). The DNA isolation kit was purchased from QIAamp (DNA Mini kit, Germany). An ELISA kit for HBV antigen analysis was purchased from Wanti-Biopharm (Beijing). All other reagents used were of analytical or chromatographic grade.
8
4T1 murine breast cancer cell culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell culture and chemicals. 4T1 murine breast carcinoma cells with stably transfected multi-cistronic reporter genes have been reported previously, so called 4T1_PB3R (24) . Cells were cultured in RPMI1640 medium (Gibco; Invitrogen Inc., Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS; HyClone; Thermo, Waltham, MA, USA), 1% penicillin-streptomycin solution (Caisson Laboratories Inc., North Logan, UT, USA) and 1% l-glutamine (200 mM) (Sigma-Aldrich Co., St. Louis, MO, USA). Cells were cultured in a 37˚C, humidified incubator containing 95% air and 5% CO 2 and passaged every 2 days. Preparation of glycated chitosan (10 mg/ml, dissolved in deionized distilled water) has been described previously (16) . It was then stored in the refrigerator until used.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!