Maleimide-PEG2000-COOH was purchased from Shanghai Yare Biotech Inc, (Shanghai, People’s Republic of China). Human Tf (iron-free) and L-α-phosphatidylethanolamine (PE) were purchased from Sigma-Aldrich Corp (St Louis, MO, USA). Tf-PEG-PE was synthesized by our group. 3-[4,5-dimehyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium bromide (MTT), DOX·HCl, stearic acid, dimethyldiocta-decylammonium bromide (DDAB), soybean phosphatidylcholine, triethylamine (TEA), and Tween® 80 were purchased from Sigma-Aldrich. Precirol® ATO 5 was generously provided by Gattefossé (St Priest, France). Injectable soya lecithin was obtained from Shanghai Taiwei Pharmaceutical Co, Ltd., (Shanghai, People’s Republic of China). Enhanced green fluorescence protein plasmid (pEGFP)-N1 was provided by Shandong University (Shandong, People’s Republic of China). Quant-iT™ PicoGreen® double-stranded (ds) DNA quantitation reagent was obtained from Invitrogen by Life Technologies (Carlsbad, CA, USA). All other chemicals were of analytical grade or higher.
L α phosphatidylethanolamine
Manufactured by Merck Group
Sourced in United States
L-α-phosphatidylethanolamine is a phospholipid compound that is a major component of cell membranes. It functions as a key structural element in biological membranes, contributing to their fluidity and permeability.
Automatically generated - may contain errors
Lab products found in correlation
L α phosphatidylinositol, by Merck Group (2 mentions)
Stearic acid, by Merck Group (1 mentions)
Tween 80, by Merck Group (1 mentions)
Triethylamine, by Merck Group (1 mentions)
Soybean phosphatidylcholine, by Merck Group (1 mentions)
Dox hcl, by Merck Group (1 mentions)
Human tf iron free, by Merck Group (1 mentions)
Dimethyldioctadecylammonium bromide, by Merck Group (1 mentions)
Egg pc, by Merck Group (1 mentions)
Cardiolipin, by Avanti Polar Lipids (1 mentions)
3 sn pa, by Merck Group (1 mentions)
Brain ps, by Avanti Polar Lipids (1 mentions)
1 2 dioleoyl sn glycero 3 phospho rac 1 glycerol sodium salt, by Merck Group (1 mentions)
1 2 dioleoyl sn glycero 3 phosphocholine, by Merck Group (1 mentions)
Cardiolipin, by Merck Group (1 mentions)
Tributyrin, by Merck Group (1 mentions)
Sphingomyelin, by Merck Group (1 mentions)
Tricaprylin, by Merck Group (1 mentions)
1 2 diacyl sn glycero 3 phospho l serine, by Merck Group (1 mentions)
Triolein, by Merck Group (1 mentions)
4 protocols using l α phosphatidylethanolamine
1
Lipid-Based Nanocarrier Synthesis and Characterization
2
Artificial Lipid Bilayer Membrane Fabrication
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Powder form of Egg-PC (Sigma Aldrich), L-α-phosphatidylethanolamine (Sigma Aldrich), 16:0 Cardiolipin (Avanti Polar Lipids), 3-sn-PA (Sigma Aldrich), Brain PS (Avanti Polar Lipids) was dissolved in chloroform. Next, 270 μM PC, 145 μM phosphatidylethanolamine, 65 μM PIP4, 5 μM CL, 5 μM PA, 10 μM PS were mixed and kept for overnight evaporation. The thin lipid film obtained the following day after removal of residual chloroform was dissolved in 500 μl of 20 mM Tris pH 7.5 and 150 mM NaCl with gentle pipetting. The solution was next incubated at 55 °C for 30 min. The resultant colloidal heated solution was subjected to extrusion as mentioned above. Finally, the model membrane liposomes comprising a mixture of PC, phosphatidylethanolamine, PIP4, CL, PA, and PS were used for downstream experiments and the remaining was stored at 4 °C for not more than 3 days.
3
Lipid Synthesis and Characterization
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Soybean-phosphatidylcholine (PC, 95%) was purchased from Avanti Polar Lipids (Alabaster, AL, USA). Tributyrin (TC4, 99%), Tricaprylin (TC8, 99%), Triolein (TC18, 99%), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC, ≥97%), l -α-phosphatidylinositol (PI, ≥99%), 1,2-diacyl-sn-glycero-3-phospho-l -serine (PS, ≥97%), l -α-phosphatidylethanolamine (PE, ≥99%), cardiolipin (CL, ≥98%), 3-sn-Phosphatidic acid sodium salt (PA, ≥98%), Sphingomyelin (SM, ≥98%), 1,2-dioleoyl-sn-glycero-3-phospho-rac-(1-glycerol) sodium salt (PG, ≥98%), 1,2-distearoyimonoglactosylglyceride (MGDG, ≥98%) were all from Sigma-Aldrich Corporation (St. Louis, MO, USA). Olive oil and sucrose esters were purchased from Aladdin Reagent Co. (Shanghai, China). Six pure chiral pseudoglycerides (didecanoyl-deoxyamino-O-methyl glycerol, DDGs) used in the present studies were synthesized according to the method reported before [23 (link)].
4
Comprehensive Lipid Extraction and Analysis Protocol
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Methanol, isooctane, acetic acid, butylated hydroxytoluene,
sodium
chloride, pentane, diethyl ether, hexane, and boron trifluoride (BF3) in methanol are purchased from Merck Millipore, Schiphol-Rijk,
Netherlands. PC-D70 1,2-distearoyl-D70-sn-glycero-3-phosphocholine was obtained from Avanti Polar
Lipids, Alabaster, Alabama, United States. A phospholipid mixture
for HPLC from soybean containingl -α-lysophosphatidylcholine
(7.7 weight(w)%),l -α-phosphatidylcholine from soybean
(38.5 w%),l -α-phosphatidylethanolamine (30.8 w%),
andl -α-phosphatidylinositol (23.0 w%) were purchased
from Sigma-Aldrich, St. Louis, Missouri, United States. A detailed
list of included FA with their systematic names and a list with FAMEs
and their suppliers is reported inTable 1 and Supplemental Tables S1 and S2 .
sodium
chloride, pentane, diethyl ether, hexane, and boron trifluoride (BF3) in methanol are purchased from Merck Millipore, Schiphol-Rijk,
Netherlands. PC-D70 1,2-distearoyl-D70-sn-glycero-3-phosphocholine was obtained from Avanti Polar
Lipids, Alabaster, Alabama, United States. A phospholipid mixture
for HPLC from soybean containing
(7.7 weight(w)%),
(38.5 w%),
and
from Sigma-Aldrich, St. Louis, Missouri, United States. A detailed
list of included FA with their systematic names and a list with FAMEs
and their suppliers is reported in
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