Cd19 allophycocyanin apc

Manufactured by BD

CD19-allophycocyanin (APC) is a fluorescent dye conjugate used for the detection and identification of CD19-positive cells in flow cytometry analysis. It consists of the CD19 antibody coupled to the APC fluorescent dye. APC is a bright, fluorescent protein derived from cyanobacteria that emits light in the far-red region of the visible spectrum.

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CD19-APC (88 citations) CD19-allophycocyanin (5 citations)

2 protocols using cd19 allophycocyanin apc

Multiparameter Flow Cytometry Analysis

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We used the following conjugated antibodies: CD38-fluorescein isothiocyanate, CD34-phycoerythrin (PE)-cyanin5 (Cy5), CD4-Pacific Blue, CD19-allophycocyanin (APC), CD3-Alexa Fluor 700, CD8-APC-H7, CCR7-Alexa Fluor 647, CD79a-APC from BD Biosciences (San Jose, CA); CD56-PE, CD45RA-PE-TexasRed, CD7-PE-Cy7, CD1a-PE, CD5-PE-Cy5 from Beckman Coulter (Brea, CA); CD31-PE from Miltenyi Biotech (Bergisch Gladbach, Germany) and CD27-PE-Cy7 from eBioscience (San Diego, CA). We obtained LIVE/DEAD aqua fluorescent dye from Molecular Probes (Invitrogen, Carlsbad, CA). The Fam-FLICA-FITC probe against active caspase-1 was used according to the manufacturers’ instructions (ImmunoChemistry, Bloomington, MN). Standard protocols were used for all types of staining. Data were acquired with an LSRII Flow Cytometer (BB Biosciences), and analyzed with FlowJo v7.6.5 (Treestar, Ashland, OR).

Menkova-Garnier I., Hocini H., Foucat E., Tisserand P., Bourdery L., Delaugerre C., Benne C., Lévy Y, & Lelièvre J.D. (2016). P2X7 Receptor Inhibition Improves CD34 T-Cell Differentiation in HIV-Infected Immunological Nonresponders on c-ART. PLoS Pathogens, 12(4), e1005571.

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Immunophenotypic Characterization of iMSCs

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iMSC surface antigens were analyzed using a FACSCalibur flow cytometer (BD Biosciences) employing FlowJo software version 10 (Tree Star, Ashland, OR, USA). The following conjugated monoclonal antibodies (BD Biosciences) were used at concentrations recommended by the manufacturer: CD34-phycoerythrin (PE) (Cat#12-0349-42), CD31-fluorescein isothiocyanate (FITC) (Cat#11-0319-42), CD19-allophycocyanin (APC) (Cat#152409), CD11-FITC (Cat#11-0112-41), human leukocyte antigen–DR isotype (HLA-DR)-FITC (Cat#11-9956-41), CD44-FITC (Cat#11-0441-81), CD73-FITC (Cat#11-0739-41), CD105-FITC (Cat#12-1051-81), and CD90-FITC (Cat#11-0909-41). We also prepared appropriate isotype staining controls.

Lim S.W., Kim K.W., Kim B.M., Shin Y.J., Luo K., Quan Y., Cui S., Ko E.J., Chung B.H, & Yang C.W. (2021). Alleviation of renal ischemia/reperfusion injury by exosomes from induced pluripotent stem cell-derived mesenchymal stem cells. The Korean Journal of Internal Medicine, 37(2), 411-424.

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