Application suite for advanced fluorescence las af version 2.35 software

Manufactured by Leica

Leica Application Suite for Advanced Fluorescence (LAS AF; version 2.35) is a software package designed for the acquisition, processing, and analysis of fluorescence microscopy data. It provides a comprehensive suite of tools for controlling Leica's advanced fluorescence imaging systems and managing the resulting image data.

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Lab products found in correlation

Tcs sp2, by Leica (1 mentions)

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2 protocols using application suite for advanced fluorescence las af version 2.35 software

Confocal Microscopy for Advanced Fluorescence

Cited 1 time

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Confocal microscopy was performed by using a Leica TCS SP2 inverted confocal microscope (Leica Microsystems, Wetzlar, Germany) equipped with a 63× water-corrected objective in multitrack mode (72 (link), 73 (link)). Collected images were analyzed with the Leica Application Suite for Advanced Fluorescence (LAS AF; version 2.35) software (Leica Microsystem).

Wu G., Cui X., Chen H., Renaud J.B., Yu K., Chen X, & Wang A. (2018). Dynamin-Like Proteins of Endocytosis in Plants Are Coopted by Potyviruses To Enhance Virus Infection. Journal of Virology, 92(23), e01320-18.

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Induction of Fluorescent Protein Expression

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Cells harboring pACYCD-sYFP were cultured at 37℃ for 2 h. Then IPTG with a final concentration of 1 mM was added to the induced group. The bacterium solution was observed under Confocal Microscopy after 4 h of induction.
All strains were prepared for Confocal Microscopy as stated above for fluorescence measurements and prepared as a wet mount. Confocal microscopy was performed using a Leica TCS SP2 inverted confocal microscope (Leica Microsystems, Wetzlar, Germany) equipped with a 63× water-corrected objective in multitrack mode. Collected images were analyzed with the Leica Application Suite for Advanced Fluorescence (LAS AF, version 2.35) software (Leica Microsystem).

Ye C., Chen X., Yang M., Zeng X, & Qiao S. (2021). CRISPR/Cas9 mediated T7 RNA polymerase gene knock-in in E. coli BW25113 makes T7 expression system work efficiently. Journal of Biological Engineering, 15, 22.

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