Anti human β actin ac 15 antibody

Manufactured by Merck Group

Sourced in United States

The Anti-human β-actin (AC-15) antibody is a laboratory reagent used for the detection and quantification of the β-actin protein, a commonly expressed cytoskeletal protein, in various biological samples. The antibody can be employed in techniques such as Western blotting, immunocytochemistry, and immunohistochemistry to identify and analyze the presence and levels of β-actin.

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Lab products found in correlation

Goat anti mouse igg h l, by Bio-Rad (3 mentions) Goat anti rabbit igg h l, by Bio-Rad (3 mentions) Atr n 19, by Santa Cruz Biotechnology (2 mentions) Anti human nfat1 1 nfat 1 antibody, by BD (2 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (2 mentions) P p53 ser15, by Cell Signaling Technology (1 mentions) Hydrochloric acid (hcl), by Thermo Fisher Scientific (1 mentions) Methanol, by Thermo Fisher Scientific (1 mentions) Sodium hydroxide (naoh), by Thermo Fisher Scientific (1 mentions) Polyethylenimine (pei), by Merck Group (1 mentions) Poly methacrylic acid, by Merck Group (1 mentions) Poly n vinylpyrrolidone, by Merck Group (1 mentions) 1 ethyl 3 3 dimethylamino propyl carbodiimide hydrochloride edc, by Chem-Impex (1 mentions) Sodium phosphate monobasic, by Thermo Fisher Scientific (1 mentions) Ethylenediaminetetraacetic acid edta, by Thermo Fisher Scientific (1 mentions) L glutathione reduced gsh, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Corning (1 mentions)

Spelling variants of this product

Anti-β-actin antibody (1089 citations) β-actin antibody (695 citations) Anti-β-actin (AC-15) (112 citations) β-actin (AC-15, (92 citations) Anti-human β-actin (23 citations) Anti-β-actin antibody (AC-15, (20 citations) Anti-actin AC-15 (17 citations) β-actin antibody (AC-15, (4 citations) Anti-human β-actin antibody (4 citations) Human β-actin (4 citations)

3 protocols using anti human β actin ac 15 antibody

Molecular Mechanisms of Cellular Regulation

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All chemicals and solvents used in the present study were of highest grade available. InuA with a purity higher than 95% was prepared as reported previously [34 (link)]. The antibodies against human MDM2 (Ab-2), p21 (Ab-1), and p-H2AX (Ser139) were bought from EMD Chemicals (Gibbstown, NJ, USA). The antibodies against human p53 (DO-1), Cdk2 (M2), Cdk4 (H-22), Cdk6 (C-21), cyclin D1 (DCS-6), cyclin E (HE12), c-Myc (0.N.222), Bax (N-20), Bcl-2 (100), PARP (H-250), Chk1 (G-4), Chk2 (B-4), and ATR (N-19) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The antibodies against human p-Chk1 (Ser317), p-Chk2 (Thr68), and p-p53 (Ser15) were obtained from Cell Signaling Technology (Danvers, MA, USA). The anti-human NFAT1 (1/NFAT-1) antibody was sourced from BD Biosciences (San Jose, CA, USA) and the anti-human β-actin (AC-15) antibody was purchased from Sigma (St. Louis, MO, USA). The goat anti-mouse IgG (H+L) and goat anti-rabbit IgG (H+L) antibodies were bought from Bio-Rad (Hercules, CA, USA). The NFAT1 and MDM2 siRNA pool and control siRNA pool were obtained from Thermo Scientific (Rockford, IL, USA).

Qin J.J., Wang W., Sarkar S., Voruganti S., Agarwal R, & Zhang R. (2016). Inulanolide A as a new dual inhibitor of NFAT1-MDM2 pathway for breast cancer therapy. Oncotarget, 7(22), 32566-32578.

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Synthesis and Characterization of Anticancer Nanoparticles

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Poly(methacrylic acid) (PMAA, average M_w 21800 g mol⁻¹), poly(N-vinylpyrrolidone) (PVPON, average M_w 58000 g mol⁻¹), poly(ethylenimine) (PEI, average M_w 25000 g mol⁻¹), were purchased from Sigma-Aldrich. 1-Ethyl-3-(3-(dimethylamino)propyl)-carbodiimide hydrochloride (EDC) was obtained from Chem-Impex International. Monobasic sodium phosphate, hydrochloric acid, sodium hydroxide, L-glutathione reduced (GSH), ethylenediaminetetraacetic acid (EDTA) and methanol were from Fisher Scientific and used as received. Ultrapure deionized water with a resistivity of 18 MΩ cm was used in all experiments (Evoqua). The anti-human p53 (DO-1) antibody was from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). The anti-human MDM2 (Ab-2) and p21 (Ab-1) antibodies were from EMD Chemicals, Inc. (Gibbstown, NJ, USA). The anti-human β-actin (AC-15) antibody was from Sigma (St. Louis, MO, USA). The goat anti-mouse IgG (H+L) and goat anti-rabbit IgG (H+L) were obtained from Bio-Rad (Hercules, CA, USA). Fetal bovine serum was obtained from Atlanta Biologicals (Lawrenceville, GA, USA). Penicillin/streptomycin was from Corning (Manassas, VA, USA). Porous cubic Mn₂O₃ microparticles (1.5–2 μm) were synthesized as described previously [37 ]. Anti-cancer drug BA-TPQ was synthesized as described previously [47 ] (see Supplementary Material for details).

Xue B., Wang W., Qin J.J., Nijampatnam B., Murugesan S., Kozlovskaya V., Zhang R., Velu S.E, & Kharlampieva E. (2017). Highly efficient delivery of potent anticancer iminoquinone derivative by multilayer hydrogel cubes. Acta biomaterialia, 58, 386-398.

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Reagents and Antibodies for Protein Analysis

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LinA was prepared as described in our previous study^{[48 ]}. All chemicals and solvents used in the present study were of highest grade available. Fetal bovine serum (FBS) was obtained from Atlanta Biologicals (Lawrenceville, GA, USA). The penicillin/streptomycin preparation was bought from Corning (Manassas, VA, USA). The anti-human NFAT1 (1/NFAT-1) antibody was purchased from BD Biosciences (San Jose, CA, USA). The antibodies against human MDM2 (Ab-2), p21 (Ab-1), and p-H2AX (Ser139) were sourced from EMD Chemicals (Gibbstown, NJ, USA). The antibodies against human p53 (DO-1), Cdk2 (M2), Cdk4 (H-22), Cdk6 (C-21), cyclin D1 (DCS-6), cyclin E (HE12), c-Myc (0.N.222), Bax (N-20), Bcl-2 (100), PARP (H-250), Chk1 (G-4), Chk2 (B-4), and ATR (N-19) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The antibodies against human p-Chk1 (Ser317), p-Chk2 (Thr68), and p-p53 (Ser15 (link)) were purchased from Cell Signaling Technology (Danvers, MA, USA). The anti-human β-actin (AC-15) antibody was bought from Sigma (St. Louis, MO, USA) and the goat anti-mouse IgG (H+L) and goat anti-rabbit IgG (H+L) antibodies were obtained from Bio-Rad (Hercules, CA, USA).

Qin J.J., Sarkar S., Voruganti S., Agarwal R., Wang W, & Zhang R. (2016). Identification of lineariifolianoid A as a novel dual NFAT1 and MDM2 inhibitor for human cancer therapy. Journal of Biomedical Research, 30(4), 322-333.

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