Ctvox v3

Manufactured by Bruker

Sourced in United States

CTvox v3.3.0 is a software application developed by Bruker for the visualization and analysis of 3D computed tomography (CT) data. The software provides tools for image processing, data exploration, and visualization of CT scans.

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Lab products found in correlation

Skyscan 1172, by Bruker (3 mentions) Ct analyser ctan v 1, by Bruker (2 mentions) Nrecon v1, by Bruker (2 mentions) Skyscan 1272, by Bruker (1 mentions)

Spelling variants of this product

CTVox (120 citations) CTVox v3.3 program (2 citations)

6 protocols using ctvox v3

Micro-CT Imaging of Nerve Segments

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Each nerve trunk was sectioned into several 6 cm-long segments and the rostral end of each segment was marked with a suture knot, to maintain the rostral-caudal direction. Each nerve segment was scanned individually in the micro-CT scanner. Nerve segments were mounted in position on a vertical sample holder tube. The samples were scanned using Bruker micro-CT Skyscan 1172 with a voxel size of 6 μm. Volume rendering was done using Bruker CTvox v3.3.1 to obtain some 3D views of the nerve (Suppl. Figs. S2-S4).

Jayaprakash N., Song W., Toth V., Vardhan A., Levy T., Tomaio J., Qanud K., Mughrabi I., Chang Y.C., Rob M., Daytz A., Abbas A., Nassrallah Z., Volpe B.T., Tracey K.J., Al-Abed Y., Datta-Chaudhuri T., Miller L., Barbe M.F., Lee S.C., Zanos T.P, & Zanos S. (2023). Organ- and function-specific anatomical organization of vagal fibers supports fascicular vagus nerve stimulation. Brain stimulation, 16(2), 484-506.

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Micro-CT Imaging of Nerve Segments

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Burtscher M., Likar R., Nachbauer W, & Philadelphy M. (1998). Aspirin for prophylaxis against headache at high altitudes: randomised, double blind, placebo controlled trial. BMJ : British Medical Journal, 316(7137), 1057-1058.

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Femur Microstructure Analysis Protocol

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Soft tissue and pin-free femurs were scanned within 2 h of dissection using a Bruker Skyscan 1276 (Bruker Preclinical Imaging) with a source voltage of 85 kV, a source current of 200 μA, a filter setting of AI 1 mm, and pixel size of 12 μm at 2016 × 1344. Phantom targets provided by the manufacturer were used to calibrate instrument measurements. Reconstructed samples were analysed using CT Analyser (CTan) v.1.17.7.2 and CTvox v.3.3.0 software (Bruker). Trabecular bone parameters of uninjured femur bones were assessed by analysing a region of 200 sections that was defined 50 sections distal of the end of the growth plate. Fracture calluses were analysed by selecting 150 sections in both directions of the fracture gap, yielding a total area of 300 sections. The exact region spanning fracture callus outside the boundaries of cortical bone was then manually selected using CTAn for analyses.

Ambrosi T.H., Marecic O., McArdle A., Sinha R., Gulati G.S., Tong X., Wang Y., Steininger H.M., Hoover M.Y., Koepke L.S., Murphy M.P., Sokol J., Seo E.Y., Tevlin R., Lopez M., Brewer R.E., Mascharak S., Lu L., Ajanaku O., Conley S.D., Seita J., Morri M., Neff N.F., Sahoo D., Yang F., Weissman I.L., Longaker M.T, & Chan C.K. (2021). Aged skeletal stem cells generate an inflammatory degenerative niche. Nature, 597(7875), 256-262.

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Quantitative Bone Graft Mineralization

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Grafts were dissected from mice and fixed in 2% PFA overnight. The next day grafts were transferred to tubes containing sterile water and scanned using a Bruker Skyscan 1276 (Bruker Preclinical Imaging) with a source voltage of 85 kV, a source current of 200 µA, a filter setting of AI 1 mm, and pixel size of 12 microns at 2016 x 1344. Reconstructed samples were analyzed using CT Analyser (CTan) v1.17.7.2 and CTvox v3.3.0 software (Bruker). Sections spanning the size of the graft were selected and upper (255) and lower (60) grey threshold were set. The total mineralized volume was measured for each graft assuming equal starting amounts of anorganic cancellous bone graft granulat.

Goodnough L.H., Ambrosi T.H., Steininger H.M., Butler M.G., Hoover M.Y., Choo H., Van Rysselberghe N.L., Bellino M.J., Bishop J.A., Gardner M.J, & Chan C.K. (2022). Cross-species comparisons reveal resistance of human skeletal stem cells to inhibition by non-steroidal anti-inflammatory drugs. Frontiers in Endocrinology, 13, 924927.

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Micro-CT Scanning of Teeth

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All teeth were scanned by means of a µCT scanner (Skyscan 1172, Bruker Skyscan, Kontich, Belgium) using the following parameters: 100 kV/100 µA X-ray source, 0.5-mm aluminum filter, pixel size of 9.92 µm, averaging frame of 7, rotation step of 0.3°, random movement of 50 and 360° rotation around the vertical axis. The raw data of all specimens was reconstructed using NRecon v.1.6.9.18 software (Bruker Skyscan) and the image processing and analysis were performed with FiJi ImageJ software (v 1.52p) and CT-Analyser (CTAn v. 1.18.8.0, Bruker Skyscan). The 3D model rendering was made using CT Vox (CTvox, v.3.3.0, Bruker Skyscan).

Pedano M.S., Yoshihara K., Li X., Camargo B., Van Landuyt K, & Van Meerbeek B. (2021). Experimental resin-modified calcium-silicate cement containing N-(2-hydroxyethyl) acrylamide monomer for pulp tissue engineering. Materials science & engineering. C, Materials for biological applications, 126.

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Hydrogel Morphological Analysis by SEM and μCT

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The hydrogels were lyophilized after solvent exchange with ethanol, followed by transfer to tert-BuOH. Dried samples were coated with platinum by vacuum evaporation (MSP-20UM, Vacuum Device, Mito-shi, Ibaraki, Japan) and observed by Scanning Electron Micrography (SEM) (Keyence VE-9800 SEM, Keyence, Osaka, Japan) at 10 kV. The average pore size was estimated by SEM photographs using ImageJ (v1.53a NIH, Bethesda, MD, USA).
The porosity of the hydrogels was estimated by µCT measurement. The measurement was performed by an X-ray Micro-tomograph apparatus (Skyscan-1272, Bruker, Billerica, MA, USA) at 5 µm/pixel resolution. Data sets were reconstructed (NRecon v1.7.4.6, Bruker, USA) and representative segments were transformed into binary images with dynamic thresholds and applied for morphometric analysis (CT Analyzer v1.18.8.0+, Bruker) and for making 3-dimensional models (CTvox v3.3.0, Bruker). The porosity was calculated during the morphometric analysis process by CT Analyzer.

Burger D., Beaumont M., Rosenau T, & Tamada Y. (2020). Porous Silk Fibroin/Cellulose Hydrogels for Bone Tissue Engineering via a Novel Combined Process Based on Sequential Regeneration and Porogen Leaching. Molecules, 25(21), 5097.

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