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Hdac2 sc 7899

Manufactured by Santa Cruz Biotechnology
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Hdac2 (sc-7899) is a lab equipment product offered by Santa Cruz Biotechnology. It is an antibody that recognizes the HDAC2 protein, which is a histone deacetylase enzyme involved in the regulation of gene expression.

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Lab products found in correlation

Ab1765, by Abcam (1 mentions) Pvdf membrane, by Thermo Fisher Scientific (1 mentions) Chd4 a301 081a, by Fortis Life Sciences (1 mentions) Nupage 4 12 bis tris gel, by Thermo Fisher Scientific (1 mentions) Transfer buffer, by Thermo Fisher Scientific (1 mentions) Rbbp4, by Abcam (1 mentions) Sall4, by Abcam (1 mentions) Nitrocellulose membrane, by Bio-Rad (1 mentions)

2 protocols using hdac2 sc 7899

1

Protein Complex Analysis via Gel Electrophoresis

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For denaturing PAGE, samples were separated in NuPAGE Bis-Tris 4–12% gels (Life Technologies) using MOPS running buffer according to manufacturer's instructions. Gels were transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA) using XCell Transfer Kit and Transfer Buffer (Life Technologies) according to manufacturer's instructions. BN-PAGE gels were first incubated in 8% acetic acid prior to transfer to PVDF membranes (Life Technologies). Protein detection was performed using the following antibodies: FLAG (M2-peroxidase labeled, A8592, Sigma-Aldrich), Sall4 (ab29112, Abcam, Cambridge, UK), Chd4 (A301–081A, Bethyl Laboratories Inc., Montgomery, TX), Hdac2 (sc-7899, Santa Cruz, Biotechnology Inc., Dallas, TX), Wdr5 (A302–430A, Bethyl Laboratories Inc.), Wdr5 (A302–429A, Bethyl Laboratories Inc.), and Rbbp4 (ab1765, Abcam).
Bode D., Yu L., Tate P., Pardo M, & Choudhary J. (2015). Characterization of Two Distinct Nucleosome Remodeling and Deacetylase (NuRD) Complex Assemblies in Embryonic Stem Cells. Molecular & Cellular Proteomics : MCP, 15(3), 878-891.
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2

Cell Lysis and Protein Analysis

Cited 1 time
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Cell lysis, immunoprecipitation, cell fractionation and EMSA were performed as described (18 (link), 20 (link), 21 (link), 30 (link), 31 (link)). Jak1 (#3332), Jak2 (#3230), phospho-STAT3 (#9145), STAT3 (#9139) antibodies were from Cell Signaling Technologies. Actin (sc-8432), p65 (sc-372), c-Myc(9E10) (sc-40), and HDAC2 (sc-7899) antibodies were from Santa Cruz Biotechnologies. FLAG (M2) beads (A2220) were from Sigma-Aldrich. USP6 antibody was described previously (20 (link)).
Quick L., Young R., Henrich I.C., Wang X., Asmann Y.W., Oliveira A.M, & Chou M.M. (2016). Jak1-STAT3 signals are essential effectors of the USP6/TRE17 oncogene in tumorigenesis. Cancer research, 76(18), 5337-5347.
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