Lung samples were formalin- or Zn- fixed followed by paraffin embedding and immunostaining of 5 µm sections as previously described (21 (link)). H&E staining were preformed as reported before (23 (link)). Sections were stained with the following antibodies: anti-MCP-1 (BD Biosciences), anti-CD86 (BD Biosciences), anti-CD3 (Epitomics), anti-PD-1 (clone 29F.1A12, Biolegend), anti-PDL-1 (clone 10F.9G2, Biolegend), anti-granzyme B (Abcam) and anti-VEGF (Thermo Scientific).
Anti mcp 1
Manufactured by BD
Anti-MCP-1 is a laboratory reagent used to detect and quantify the presence of monocyte chemoattractant protein-1 (MCP-1) in biological samples. MCP-1 is a chemokine that plays a role in the recruitment and activation of monocytes and macrophages. The Anti-MCP-1 reagent can be used in various analytical techniques, such as ELISA, to measure MCP-1 levels in research applications.
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Lab products found in correlation
Anti granzyme b, by Abcam (1 mentions)
Anti vegf, by Thermo Fisher Scientific (1 mentions)
Anti cd86, by BD (1 mentions)
Anti pd 1 clone 29f 1a12, by BioLegend (1 mentions)
Anti pd l1 clone 10f 9g2, by BioLegend (1 mentions)
Bicinchoninic acid (bca), by Beyotime (1 mentions)
Nitrocellulose membrane, by Merck Group (1 mentions)
Anti nf κb p65, by Abcam (1 mentions)
2 protocols using anti mcp 1
1
Comprehensive Immunohistochemical Analysis of Lung Samples
2
Protein Expression Analysis in HRGECs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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HRGECs were lysed in RIPA buffer and stored at -80°C after protein concentration determination using BCA (Beyotime Biotech). SDS-PAGE with a 5% stacking gel and a 12% resolving gel to was used to resolve proteins by molecular weight. Resolved proteins were transferred onto nitrocellulose membranes (Millipore Corp). The primary antibodies used were: anti-NF-κB p65 (ABCAm), anti-eNOS (CST), anti-MCP-1 (BD). GAPDH was used as the loading control.
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