Nunclon δ surface multidishes

Normal adult human epithelial keratinocytes (HEKa) were obtained from ScienCell Research Laboratories (San Diego, CA). The defined proprietary culture medium, CNT-Prime, was obtained from Cellntec Advanced Cell Systems AG (Bern). Goat serum, trypsin-ethylenediaminetetraacetic acid (EDTA), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), sodium bicarbonate, sodium azide, Tween-20, Triton X-100, gentamicin, bovine serum albumin (BSA), fetal bovine serum (FBS), 4′,6-diamidino-2-phenylindole (DAPI), dispase II, soybean trypsin inhibitor, DHR, DHE, menadione and trypan blue were purchased from Sigma Aldrich (St Louis, MO). Nunclon Δ surface multidishes, glass coverslips, pipettes and other routine plastics were obtained from Thermo Fisher Scientific (Waltham, MA). Phosphate buffered saline (PBS), Hanks balanced salt solution (HBSS) and minimum essential medium (MEM) were from Life Technologies (Carlsbad, CA).

Normal adult human epithelial keratinocytes (HEKa) and Keratinocyte Medium (KM) were obtained from ScienCell Research Laboratories (San Diego, CA). The provided cells originated from an 18 year old female undergoing breast-reduction surgery. Goat serum, trypsin-ethylenediaminetetraacetic acid (EDTA), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), sodium bicarbonate, sodium azide, Tween-20, Triton X-100, gentamycin, bovine serum albumin (BSA), fetal bovine serum (FBS), 4', 6-diamidino-2-phenylindole (DAPI), were purchased from Sigma Aldrich (St Louis, MO). Nunclon Δ surface multidishes, glass coverslips, pipettes and other routine plastics were obtained from Thermo Fisher Scientific (Waltham, MA). Phosphate buffered saline (PBS) and minimum essential medium (MEM) were from Life Technologies (Carlsbad, CA).

Proprietary cell culture medium CnT-Prime was purchased from Cellntec Advanced Cell Systems AG (Bern). Trypsin-ethylenediaminetetraacetic acid (EDTA), soybean trypsin inhibitor, sodium bicarbonate, goat serum, sodium azide, Tween-20, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), Triton X-100, gentamicin, bovine serum albumin (BSA), fetal bovine serum (FBS), penicillin-streptomycin, human recombinant insulin, hydrocortisone, adenine, 3,3,5-triiodo-L-thyronine (T3), dispase II, and trypan blue were purchased from Sigma Aldrich (St Louis, MO). Cholera toxin was purchased from Enzo Life Sciences (Farmingdale, USA). Epidermal growth factor, Gibco low-glucose Dulbecco’s modified Eagle’s medium (DMEM), Gibco 1:1 DMEM/F12 nutrient mix, minimal essential medium (MEM), phosphate buffered saline (PBS), Hank’s balanced salt solution, routine plastics, pipettes and Nunclon Δ surface multi-dishes were purchased from Thermo Fisher Scientific (Life Technologies) (Waltham, MA).